上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (12): 1513-.doi: 10.3969/j.issn.1674-8115.2010.12.016

• 论著（基础研究） • 上一篇    下一篇

苯并[a]芘对宫颈癌HeLa细胞细胞色素P450 1A1的诱导作用

石玉荣1,2, 耿 建1,2, 王 惠2, 章 尧3   

  1. 1.蚌埠医学院生物化学与分子生物学教研室, 蚌埠 233000；2.蚌埠医学院检验诊断学实验中心, 蚌埠 233000；3.皖南医学院生物化学教研室, 芜湖 241002
  • 出版日期:2010-12-25 发布日期:2010-12-31
  • 通讯作者: 章 尧, 电子信箱: zhangrao@ahedu.gov.com。
  • 作者简介:石玉荣（1974—）, 女, 讲师, 硕士；电子信箱: shiyr@126.com。
  • 基金资助:

    安徽省高等学校青年教师科研资助项目（2008jq1107）

Effects of benzo(a)pyrene on expression of cytochrome P450 1A1 in HeLa cells of uterine cervix cancer

SHI Yu-rong1,2, GENG Jian1,2, WANG Hui2, ZHANG Yao3   

  1. 1.Department of Biochemistry and Molecular Biology, 2.Research Center of Clinical Laboratory Science, Bengbu Medical College, Bengbu 233000, China；3.Department of Biochemistry, Wannan Medical College, Wuhu 241002, China
  • Online:2010-12-25 Published:2010-12-31
  • Supported by:

    Research Foundation for Young Teachers in Colleges of Anhui Province, 2008jq1107

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摘要：

目的 探讨苯并[a]芘（BaP）对宫颈癌HeLa细胞增殖及细胞色素P450 1A1（CYP1A1）表达的影响。方法 不同浓度(0、1.0、2.5、5.0、7.5和10.0 μmol/L)的BaP处理宫颈癌HeLa细胞，MTT法检测BaP对HeLa细胞增殖的影响，Western blotting和RT-PCR分别检测CYP1A1的蛋白表达和mRNA表达，荧光素标记检测CYP1A1活性。结果 MTT法检测显示，各浓度BaP组光密度（D553 nm）均较对照组（0 μmol/L BaP）升高，其中7.5 μmol/L BaP组最高（P<0.05）。Western blotting和RT-PCR检测结果显示，各浓度BaP组CYP1A1 mRNA及蛋白的表达水平均高于对照组，其中7.5 μmol/L BaP组最高（P<0.05）。CYP1A1活性检测结果显示，各浓度BaP组HeLa细胞CYP1A1酶活力均高于对照组，以5.0 μmol/L BaP组活力最高（P<0.05）。结论 BaP可诱导HeLa细胞增殖，诱导HeLa细胞表达CYP1A1，并增加酶活性。

关键词: 苯并[a]芘, 细胞色素P450 1A1, HeLa细胞

Abstract:

Objective To investigate the effects of benzo(a)pyrene (BaP) on the proliferation of HeLa cells of uterine cervix cancer and expression of cytochrome P450 1A1 (CYP1A1) in HeLa cells of uterine cervix cancer. Methods HeLa cells of uterine cervix cancer were treated with different concentrations of BaP (0, 1.0, 2.5, 5.0, 7.5 and 10.0 μmol/L). The effect of BaP on the proliferation of HeLa cells was detected by MTT assay, the expression of CYP1A1 protein and mRNA was determined by Western blotting and RT-PCR, respectively, and the activity of CYP1A1 was measured by fluorescein labeling. Results MTT assay revealed that optical density (D553 nm) of groups of different concentrations of Bap was higher than that of control group (0 μmol/L BaP), with the highest value in 7.5 μmol/L BaP group (P<0.05). RT-PCR and Western blotting indicated that the expression of CYP1A1 mRNA and protein of groups of different concentrations of Bap was higher than that in control group, with the highest value in 7.5 μmol/L BaP group (P<0.05). The activity of CYP1A1 in groups of different concentrations of BaP was higher than that in control group, with the highest value in 5.0 μmol/L BaP group (P<0.05). Conclusion BaP can promote the proliferation of HeLa cells, and increase the expression and activity of CYP1A1 in HeLa cells.

Key words: benzo(a)pyrene, cytochrome P450 1A1, HeLa cells