上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (10): 1492-.doi: 10.3969/j.issn.1674-8115.2011.10.030

• 短篇论著 • 上一篇    下一篇

上海地区大米样品黄曲霉素B1含量检测

叶 露, 韦艳霞, 刘 畅   

  1. 上海交通大学 基础医学院病原生物学教研室, 上海 200025
  • 出版日期:2011-10-28 发布日期:2011-10-27
  • 通讯作者: 刘 畅, 电子信箱: tiantianlc@sjtu.edu.cn。
  • 作者简介:叶 露（1989—）, 女, 博士生；电子信箱: walawala511@gmail.com。

Detection of amount of Aflatoxin B1 in rice samples in Shanghai

YE Lu, WEI Yan-xia, LIU Chang   

  1. Department of Medical Microbiology and Parasitology, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2011-10-28 Published:2011-10-27

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摘要：

目的 对上海市区内出售的大米样本进行黄曲霉素B1含量的测定和分析。方法 以在上海市区内的散装粮食店、中型超市、大型超市和淘宝网店采购的苏北产大米和东北产大米作为受试样本,采用酶联免疫吸附法测定样本大米中黄曲霉素B1的含量。采用独立样本T检验分析不同产地样本大米中黄曲霉素B1含量的差异；单因素方差分析比较不同购物来源样本大米中黄曲霉素B1的含量差异。结果 60个受试样本中黄曲霉素B1含量的总体均值为1.926 ppb，其中超过国家标准样本数为2个，分别为购自散装店和中型超市的东北产大米；苏北和东北样本大米中黄曲霉素B1的含量分别为（1.221±1.055）ppb和（2.631±2.903）ppb，两者差异具有统计学意义（P<0.05）。散装店、网店、中型超市和大型超市样本大米中黄曲霉素B1的含量分别为（2.900±2.457）ppb、（1.570±1.416）ppb、（1.743±2.683）ppb和（0.569±0.608）ppb，四者比较差异有统计学意义（P<0.05），进一步的多重检验表明散装店与大型超市来源样本大米中黄曲霉素B1的含量存在统计学差异（P<0.05）。结论 上海市区出售的大米样本中黄曲霉素B1含量总体较低。受试样本中的黄曲霉素B1的含量，东北产大米高于苏北产大米，散装粮食店来源大米高于大型超市来源大米。在散装店和中型超市的受试样本中发现黄曲霉素B1含量超标样本。

关键词: 黄曲霉素B1, 大米, 酶联免疫吸附法

Abstract:

Objective To determine and analyse the amount of Aflatoxin B1 in rice samples sold in Shanghai. Methods Subei and Dongbei rice samples were obtained from groceries, medium-size markets, supermarkets and e-shops, and the amount of Aflatoxin B1 in rice samples was determined by enzyme linked immunosorbent assay. The amount of Aflatoxin B1 in Subei and Dongbei rice samples was compared by T test, and the amount of Aflatoxin B1 in rice samples obtained through different methods was compared by one-way analysis of variance. Results The mean amount of Aflatoxin B1 in all the 60 samples was 1.926 ppb. The amount of Aflatoxin B1 in 2 samples was higher than the national standard, which were Dongbei rice samples obtained from a grocery and a medium-size market. The amount of Aflatoxin B1 in Subei rice and Dongbei rice samples was (1.221±1.055) ppb and (2.631±2.903) ppb respectively, with significant differences between them (P<0.05). The amount of Aflatoxin B1 in rice samples obtained from groceries, e-shops, medium-size markets and supermarkets was (2.900±2.457) ppb, (1.570±1.416) ppb, (1.743±2.683) ppb and (0.569±0.608) ppb respectively, with significant differences among them (P<0.05). Multiple test revealed that there were significant differences in the amount of Aflatoxin B1 between rice samples obtained from groceries and those obtained from supermarkets (P<0.05). Conclusion The amount of Aflatoxin B1 in rice samples sold in Shanghai is relatively lower. The amount of Aflatoxin B1 in Dongbei rice samples is higher than that in Subei rice samples, and the amount of Aflatoxin B1 in rice samples obtained from groceries is higher than that obtained from supermarkets. Unqualified rice samples are obtained from a grocery and a medium-size market.

Key words: Aflatoxin B1, rice, enzyme linked immunosorbent assay