上海交通大学学报（医学版）

›› 2012, Vol. 32 ›› Issue (5): 576-.doi: 10.3969/j.issn.1674-8115.2012.05.009

• 论著（基础研究） • 上一篇    下一篇

Copolymer 1保护高眼压大鼠视神经的免疫学机制

孙静芬, 王 玲   

  1. 上海交通大学 医学院附属瑞金医院眼科, 上海 200025
  • 出版日期:2012-05-28 发布日期:2012-06-01
  • 作者简介:孙静芬（1967—）, 女, 副主任医师, 博士；电子信箱: sunjingfen@sohu.com。
  • 基金资助:

    国家自然科学基金（60978030）

Immunological mechanism of neuroprotection function of Copolymer 1 in elevated intraocular pressure in rats

SUN Jing-fen, WANG Ling   

  1. Department of Ophthalmology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
  • Online:2012-05-28 Published:2012-06-01
  • Supported by:

    National Natural Science Foundation of China, 60978030

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摘要：

目的 探讨Copolymer 1 （Cop 1）对实验性高眼压性青光眼视神经保护作用的相关免疫学机制。方法 对27只大鼠采用烧灼巩膜上静脉制作双眼高眼压模型，在眼压升高的第3周，取12只模型大鼠于后脚皮内注射100 μg Cop 1和等体积的完全弗氏佐剂（CFA）混合乳化液（实验组）；另取12只模型大鼠作为对照组，后脚皮内注射等体积PBS和CFA的混合乳化液；其余3只模型大鼠作为空白对照组，不做任何处理。1周后取出大鼠脾淋巴细胞，采用流式细胞仪检测T细胞亚群的变化；ELISA法检测T细胞分泌细胞因子的变化；[3H]thymidine标记检测淋巴细胞的增殖。结果 实验组大鼠的CD4+CD25+Treg/CD4+比值显著高于对照组和空白对照组（P<0.05）。实验组IL-10分泌量和分泌浓度均显著高于对照组和空白对照组，差异有统计学意义（P<0.05），但三组间INF-γ分泌量和分泌浓度比较差异均无统计学差异（P>0.05）。三组间体外刺激淋巴细胞指数比较差异无统计学意义（P>0.05）。结论 Cop 1 能诱导CD4+CD25－向CD4+CD25+Treg转化，促进Th1转化为Th2，并增加IL-10的分泌。

关键词: Copolymer 1, 高眼压, 视神经保护, 免疫学

Abstract:

Objective To explore the immunological mechanism of neuroprotection function of Copolymer 1 (Cop 1) in rat chronic elevated intraocular pressure model. Methods Elevated intraocular pressure model of both eyes were established in 27 rats by cauterization of episcleral veins. Three weeks after elevation of intraocular pressure, 12 rats were immunized by intracutaneous injection of mixture of Cop 1 (100 μg) emulsified in complete Freund's adjuvant （CFA）at two hind footpads (experiment group), another 12 rats were injected with the same volume of PBS emulsified in CFA (control group), and the other 3 rats received no treatment (blank control group). One week later, lymphocytes from spleen were isolated, T cell subset was detected by flow cytometry, cytokine secretion of T cells was determined by ELISA, and the proliferation of lymphocytes was examined by [3H]thymidine labeling. Results CD4+CD25+Treg/CD4+ in experiment group was significantly higher than those in control group and blank control group (P<0.05). The quantity and concentration of IL-10 secretion in experiment group were significantly higher than those in control group and blank control group （P<0.05）, while there was no significant difference in the quantity and concentration of INF-γ secretion among groups (P>0.05). There was no significant difference in in vitro proliferation index of lymphocytes among three groups (P>0.05). Conclusion Cop 1 promotes transfer of CD4+CD25－ to CD4+CD25+Treg, development of Th1 to Th2 and production of IL-10.

Key words: Copolymer 1, elevated intraocular pressure, neuroprotection, immunology