上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2013, Vol. 33 ›› Issue (6): 786-.doi: 10.3969/j.issn.1674-8115.2013.06.018

• 论著（基础研究） • 上一篇    下一篇

吗啡、舒芬太尼和地佐辛对大鼠肠道平滑肌收缩和运动功能的影响

卞小翠1，周仁龙1，闻大翔1，杭燕南1，胡优敏2   

  1. 上海交通大学 1.医学院附属仁济医院麻醉科， 上海 200001； 2.基础医学院功能学教学实验室， 上海 200025
  • 出版日期:2013-06-28 发布日期:2013-06-28
  • 通讯作者: 闻大翔, 电子信箱: wdxrwj@126.com。
  • 作者简介:卞小翠（1987—）， 女， 硕士生; 电子信箱: bxcbianxiaocui@163.com。

Effect of morphine, sufentanil and dezocine on contraction and motor function of intestinal smooth muscle in rats

BIAN Xiao-cui1, ZHOU Ren-long1, WEN Da-xiang1, HANG Yan-nan1, HU You-min2   

  1. 1.Department of Anesthesiology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200001, China; 2.Laboratory for Physiology, Pathophysiology & Pharmacology, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2013-06-28 Published:2013-06-28

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摘要：

目的 观察吗啡、舒芬太尼和地佐辛三种阿片类药物对大鼠肠道平滑肌收缩和运动功能的影响。方法 制备大鼠离体小肠，分别加入不同浓度吗啡（5、10、30 mg/kg）、舒芬太尼（20、40、120 μg/L）和地佐辛（1.7、3.4和10.2 mg/L），记录小肠平滑肌的肌张力和收缩频率，观察加药后药物对肠肌运动的影响。将32只SD大鼠随机分为对照组（1 mL生理盐水）、吗啡组（1.04 mg/kg）、舒芬太尼组（2.08 mg/kg）和地佐辛组（1.04 mg/kg），测定大鼠腹腔注射三种药物后小肠推进运动的变化。结果 10、30 mg/L吗啡和40、120 μg/L舒芬太尼作用于离体肠肌后，大鼠小肠的平滑肌肌张力均明显升高（P<0.05）；但5 mg/L吗啡和20 μg/L舒芬太尼以及1.7、3.4和10.2 mg/L的地佐辛对大鼠小肠的平滑肌肌张力均无明显作用（P>0.05）。不同浓度吗啡、舒芬太尼和地佐辛对大鼠小肠的平滑肌收缩频率均不产生明显作用（P>0.05）。与对照组比较，吗啡、舒芬太尼和地佐辛腹腔注射后小肠推进率显著减慢（P<0.05）；但三种药物组间小肠推进率比较差异均无统计学意义（P>0.05）。结论 吗啡、舒芬太尼和地佐辛对大鼠肠道运动均有抑制作用。

关键词: 吗啡, 舒芬太尼, 地佐辛, 小肠平滑肌, 阿片受体

Abstract:

Objective To investigate the effect of morphine, sufentanil and dezocine on contraction and motor function of intestinal smooth muscle in rats. Methods Isolated small intestine of rats was prepared, and were treated with different concentrations of morphine (5 mg/L, 10 mg/L and 30 mg/L), sufentanil (20 μg/L, 40 μg/L and 120 μg/L) and  dezocine (1.7 mg/L, 3.4 mg/L and 10.2 mg/L) respectively. The contractile tension and frequency of the small intestine smooth muscle of rats were recorded, and the effect of treatment was observed. Thirtytwo SD rats were randomly divided into control group (1 mL normal saline), morphine group (1.04 mg/kg), sufentanil group (2.08 mg/kg) and dezocine group (1.04 mg/kg), and the propulsive motility in intestinal tract was measured after intraperitoneal injection of three drugs in rats. Results After treatment with 10 mg/L and 30 mg/L morphine and 40 μg/L and 120 μg/L sufentanil, the contractile tension of the isolated small intestine smooth muscle of rats significantly increased (P<0.05), while 5 mg/L morphine, 20 μg/L sufentanil and different concentrations of dezocine (1.7 mg/L, 3.4 mg/L and 10.2mg/L) had no significant effect on the contractile tension of the isolated small intestine smooth muscle of rats (P>0.05). Different concentrations of morphine, sufentanil and dezocine had no significant effect on the contractile frequency of the isolated small intestine smooth muscle of rats (P>0.05). Compared with control group, the propulsive distance in intestinal tract significantly decreased after treatment with morphine, sufentanil and dezocine (P<0.05), and there was no significant difference in the propulsive distance among morphine, sufentanil and dezocine groups (P>0.05). Conclusion Morphine, sufentanil and dezocine all have an inhibitory effect on the intestinal motility of rat.

Key words: morphine, sufentanil, dezocine, small intestine smooth muscle, opioid receptor