上海交通大学学报(医学版)

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马来酸桂哌齐特预处理对大鼠脑缺血的影响

浦 政,王晓蓉,干 静,戚 辰   

  1. 上海交通大学  医学院附属新华医院神经内科, 上海 200092
  • 出版日期:2013-08-28 发布日期:2013-09-16
  • 作者简介:浦 政(1969—),男,副主任医师,博士; 电子信箱: paddy_69@126.com。
  • 基金资助:

    上海市医学会基金(SHNR-001)

Effect of cinepazide maleate preconditioning on rat models of cerebral ischemia

PU Zheng, WANG Xiao-rong, GAN Jing, QI Chen   

  1. Department of Neurology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China
  • Online:2013-08-28 Published:2013-09-16
  • Supported by:

    Shanghai Medical Association Foundation, SHNR-001

摘要:

目的 观察马来酸桂哌齐特预处理对大鼠脑缺血的影响,探讨该药物的脑保护作用。方法 将SD大鼠随机分为马来酸桂哌齐特预处理组(给药手术组)、生理盐水预处理组(对照手术组)和马来酸桂哌齐特预处理非手术组(给药非手术组),预处理5 d,给药手术组和对照手术组经改良线栓法建立大脑中动脉阻塞脑缺血模型。术后1、3、7 d时,观察各组大鼠的行为学和脑组织变化;对给药手术组和对照手术组大鼠行偏瘫评分,脑片TTC染色计算脑缺血面积,比较脑缺血严重程度。结果 术后1、3 d时,给药手术组与对照手术组大鼠的偏瘫评分和脑缺血面积比较差异均无统计学意义(P>0.05);术后7 d时,与对照手术组比较,给药手术组大鼠的偏瘫评分较低,脑缺血面积较小,差异均有统计学意义(P<0.05);给药非手术组大鼠无行为学变化,相应时间点大鼠脑组织未见缺血改变。结论 马来酸桂哌齐特可能具有脑缺血保护作用。

关键词: 马来酸桂哌齐特, 预处理, 脑缺血

Abstract:

Objective To investigate the effect of cinepazide maleate preconditioning on rat models of cerebral ischemia, and explore its neuroprotective function. Methods SD rats were randomly divided into cinepazide maleate preconditioning group (treatment-operation group), normal saline preconditioning group (control-operation group) and cinepazide maleate preconditioning without operation group (treatment-non-operation group). After preconditioning for 5 d, ischemic rat models were
established by the modified suture method of left middle cerebral artery occlusion in treatment-operation group and control-operation group. One day, 3 d
and 7 d after operation, the changes of behaviors and brain tissues of rats in each group were observed. In treatment-operation group and control-operation
group, the hemiplegia status was scored, areas of cerebral ischemia were calculated with TTC staining of brain slices, and the severity of cerebral ischemia
was compared. Results One day and 3 d after operation, there was no significant difference in the hemiplegia score and area of cerebral ischemia between
treatment-operation group and control-operation group (P>0.05). The hemiplegia score was significantly lower, and the area of cerebral ischemia was
significantly smaller in treatment-operation group than those in control-operation group 7 d after operation (P<0.05). No behavior changes and ischemic
area of brain slices was observed in treatment-non-operation group. Conclusion Cinepazide maleate may have a neuroprotective function in cerebral ischemia.

Key words: cinepazide maleate, preconditioning, cerebral infarction