上海交通大学学报(医学版)

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5-氟尿嘧啶联合人参皂苷Rg3抑制胃癌细胞的增殖

聂 娜1,吴小翎2,罗红春3,刘 波4,刘 倩5   

  1. 1.重庆医科大学附属第一医院金山医院体检中心, 重庆 401122; 2.重庆医科大学附属第二医院消化内科, 重庆 400010; 3.重庆医科大学附属第一医院传染科, 重庆 400016; 4.重庆医科大学附属儿童医院消化内科, 重庆 400014; 5.重庆市渝北区人民医院消化内科, 重庆 401120
  • 出版日期:2014-05-28 发布日期:2014-05-30
  • 通讯作者: 吴小翎, 电子信箱: wuxiaoling@33141@163.cm。
  • 作者简介:聂 娜(1982—), 女, 硕士; 电子信箱: 527236911@qq.com。

Inhibitory effect of 5-fluorouracil combined with Rg3 on proliferation of gastric cancer cells

NIE Na1, WU Xiao-ling2, LUO Hong-chun3, LIU Bo4, LIU Qian5   

  1. 1.Jinshan Hospital Medical Center, the First Affiliated Hospital of Chongqing Medical University, Chongqing 401122, China; 2.Department of Gastroenterology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China; 3.Department of Infection, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; 4.Department of Gastroenterology, the Children Affiliated Hospital of Chongqing Medical University, Chongqing 400014, China; 5.Department of Gastroenterology, Yubei People's Hospital of Chongqing, Chongqing 401120, China
  • Online:2014-05-28 Published:2014-05-30

摘要:

目的 探讨5-氟尿嘧啶(5-FU)联合人参皂苷Rg3对胃癌细胞增殖的抑制作用。方法 MTT法检测5-FU联合Rg3对人胃癌细胞MGC-803和MKN-28细胞增殖的抑制作用。构建体外肿瘤球模型研究5-FU联合人参皂苷Rg3对肿瘤球的生长抑制作用;流式细胞仪检测5-FU联合人参皂苷Rg3对MKN-28细胞的凋亡诱导作用。构建胃癌裸鼠移植瘤模型,随机分为生理盐水组(阴性对照组)、5-FU组、Rg3组和5-FU+Rg3组(联合给药组);研究5-FU联合人参皂苷Rg3对裸鼠移植瘤的生长抑制作用,统计各组肿瘤的大小,绘制肿瘤生长曲线。结果 MGC-803胃癌细胞和MKN-28胃癌细胞给药48 h后,联合给药组细胞存活率显著低于其他组,差异具有统计学意义(P<0.01)。MGC-803胃癌细胞肿瘤球和MKN-28胃癌细胞肿瘤球给药7 d后,联合给药组肿瘤球体积显著小于其他组,差异具有统计学意义(P<0.01)。细胞凋亡实验结果显示:联合给药组诱导肿瘤细胞凋亡能力显著强于其他组,差异具有统计学意义(P<0.01)。荷瘤裸鼠实验结果显示:各给药组均能有效抑制肿瘤的生长,与阴性对照组比较差异具有统计学意义(P<0.01)。联合给药显著延长荷瘤裸鼠的中位生存期,差异具有统计学意义(P<0.01)。结论 5-FU与Rg3均具有抗胃癌细胞的作用,二者联合应用是一种潜在的治疗胃癌的有效手段。

关键词: 5-氟尿嘧啶, Rg3, 胃癌

Abstract:

Objective To investigate the inhibitory effect of 5-fluorouracil (5-FU) combined with Rg3 on the proliferation of gastric cancer cells. Methods The inhibitory effect of 5-FU combined with Rg3 on the proliferation of human gastric cancer cells MGC-803 and MKN-28 was investigated by the MTT assay. The in vitro tumor spheroid model was built for studying the inhibitory effect of 5-FU combined with Rg3 on the growth of tumor spheroid. The gastric xenograft tumor model of nude mice was built and mice were randomly divided into the saline group (negative control group), 5-FU group, Rg3 group, and 5-FU+Rg3 group (combined administration group). The inhibitory effect of 5-FU combined with Rg3 on the growth of xenograft tumor of nude mice was investigated. Tumor sizes of each group were measured and growth curves of tumors were plotted. Results The viability of MGC-803 cells and MKN-28 cells of the combined administration group was significantly lower than that of the other groups after being administrated by 5-FU and Rg3 for 48 h. The difference was statistically significant (P<0.01). The sizes of tumor spheroid of MGC-803 cells and MKN-28 cells of the combined administration group were significantly smaller than those of the other groups after being administrated by 5-FU and Rg3 for 7 d. The difference was statistically significant (P<0.01). The results of apoptosis tests showed that the ability of inducing apoptosis of the combined administration group was significantly stronger than that of the other groups. The difference was statistically significant (P<0.01). The results of tumor bearing nude mice tests showed that each administration group could inhibit the growth of tumors. Compared to the negative control group, the differences were statistically significant (P<0.01). The combination of 5-FU and Rg3 could prolong the median survival time of tumor bearing nude mice. The difference was statistically significant (P<0.01). Conclusion Both 5-FU and Rg3 can inhibit the growth of gastric cancer cells and the combination of 5-FU and Rg3 may be a potentially effective treatment method for gastric cancer.

Key words: 5-fluorouracil, Rg3, gastric cancer