上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

类风湿性关节炎患者滑膜组织中HIF-1α与MMPs相关性研究

翟天航，张洁，李会丹，火蓉芬，沈佰华，李宁丽   

  1. 上海市免疫学研究所， 上海 200025
  • 出版日期:2015-09-28 发布日期:2015-09-30
  • 通讯作者: 李宁丽, 电子信箱: ninglixiaoxue57@163.com。
  • 作者简介:翟天航（1990—）， 男， 硕士生; 电子信箱: tianhangzhai@163.com。
  • 基金资助:

    国家自然科学基金（81172856）；上海市科委基金（12JC1407700，12401903702）

Correlation between HIF-1α and MMPs in synovial tissues of patients with rheumatoid arthritis

ZHAI Tian-hang , ZHANG Jie, LI Hui-dan, HUO Rong-fen, SHEN Bai-hua, LI Ning-li   

  1. Shanghai Institute of Immunology, Shanghai 200025, China
  • Online:2015-09-28 Published:2015-09-30
  • Supported by:

    National Natural Science Foundation of China，81172856; Foundation of Science and Technology Commission of Shanghai Municipality， 12JC1407700，12401903702

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摘要：

目的  探讨类风湿性关节炎（RA）患者滑膜组织中缺氧诱导因子-1α（HIF-1α）的表达与基质金属蛋白酶家族成员（MMPs）表达的相关性。方法  利用real-time PCR方法检测RA和骨关节炎(OA)患者滑膜组织中HIF-1α和MMPs的表达并进行相关性分析。结果  RA滑膜组织中HIF-1α、MMP-1、MMP-3、MMP-9与MMP-13 mRNA表达量显著高于OA对照组，且RA滑膜组织中高表达的HIF-1α与MMP-9呈显著的正相关。结论  RA滑膜组织中高表达的HIF-1α可能参与调控MMP-9的表达，参与RA的病理变化进程。

关键词: 类风湿性关节炎, 缺氧诱导因子-1α; , 基质金属蛋白酶

Abstract:

Objective  To investigate the correlation between the expression of hypoxia-inducible factor-1α (HIF-1α) and the expression of matrix metalloproteinases (MMPs) in synovial tissues of patients with rheumatoid arthritis (RA). Methods  The  expressions of HIF-1α and MMPs in synovial tissues of patients with RA and osteoarthritis (OA) were detected by real-time PCR and the correlation analysis was conducted. Results  The mRNA expressions of HIF-1α, MMP-1, MMP-3, MMP-9, and MMP-13 in synovial tissues of patients with RA were significantly higher than those of patients with OA. Highly expressed HIF-1α in synovial tissues of patients with RA significantly and positively correlated with MMP-9. Conclusion  Highly expressed HIF-1α in synovial tissues of patients with RA may involve in the regulation of the expression of MMP-9 and the process of pathological change of RA.

Key words: rheumatoid arthritis, hypoxia-inducible factor-1α, matrix metalloproteinase