上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

Ghrelin对吗啡肠麻痹小鼠结肠动力的影响

赵晶，汪艳，许涛，江伟   

  1. 上海交通大学附属第六人民医院麻醉科， 上海 200233
  • 出版日期:2015-11-28 发布日期:2016-01-13
  • 通讯作者: 江伟, 电子信箱: jiangw@sjtu.edu.cn。
  • 作者简介:赵晶（1978—）， 女， 主治医师， 硕士生; 电子信箱: 5036523@qq.com。

Effect of ghrelin on the colonic motility of morphine induced bowel dysfunction of mice

ZHAO Jing, WANG Yan, XU Tao, JIANG Wei   

  1. Department of Anesthesiology, the Sixth Peoples Hospital, Shanghai Jiao Tong University, Shanghai 200233, China
  • Online:2015-11-28 Published:2016-01-13

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摘要：

目的  探讨生长激素促分泌素受体内源性激动剂ghrelin 对吗啡肠麻痹小鼠结肠动力的影响。方法  C57小鼠50只，分别进行在体酚红推进实验和离体结肠肌条收缩实验。在体实验中，25只小鼠均分为5组，一组作为正常组，另4组建立吗啡肠麻痹模型后分别腹腔注射生理盐水和不同剂量的ghrelin（50、100和200 μg/kg）。酚红推进实验计算各组小鼠结肠推进率。离体实验中，25只小鼠亦均分为5组，分别为正常组、吗啡组和3个ghrelin治疗组（ghrelin浓度分别为0.1、1和10 μmol/L）;取小鼠结肠段，用生物信号处理系统测定各组结肠肌条自发收缩幅度。结果  在体实验中，吗啡肠麻痹小鼠的结肠推进率为(33.77±0.49)%，注射ghrelin 50、100和200 μg/kg后，结肠推进率分别为(41.42±1.66)%、(45.86±0.86)%和(56.84±0.84)%，均显著高于吗啡肠麻痹未治疗小鼠(P<0.05)。离体实验中，吗啡组小鼠的结肠肌条收缩幅度为（0.568±0.014) g;ghrelin浓度为0.1、1和10 μmol/L时，收缩幅度分别增至（0.624±0.118）、（0.673±0.014）和（0.712±0.125）g，均显著高于吗啡组(P<0.05)。结论  Ghrelin可以显著提高吗啡肠麻痹小鼠的结肠动力。

关键词: 生长激素促分泌素受体激动剂, 吗啡肠麻痹, 结肠动力

Abstract:

Objective  To investigate the effect of growth hormone secretagogue receptor agonist ghrelin on the colon motility of morphine induced bowel dysfunction of mice. Methods  Phenolsulfonphthalein pushing test in vivo and contraction test of colonic muscle strips in vitro were conducted for 50 C57 mice. For in vivo test, 25 mice were divided into five groups. One group was the control group and mice of other four groups were intraperitoneally injected with saline or different doses (50, 100, and 200 μg/kg) of ghrelin after the model of morphine induced intestinal paralysis was established. The colonic transit of mice was measured by phenolsulfonphthalein pushing test. For in vitro test, 25 mice were also divided into 5 groups, i.e. the control group, morphine group, and three ghrelin groups (ghrelin of 0.1 μmol/L,1 μmol/L, and 10 μmol/L). Mouse colons were harvested and the spontaneous contraction amplitude of colonic muscle strips was recorded by the biological signal system. Results  For in vivo test, colonic propulsive rates of the morphine group were (33.77±0.49)%. After being injected with ghrelin of 50, 100, and 200 μg/kg, colonic propulsive rates were (41.42±1.66)%, (45.86±0.86)%, and (56.84±0.84) %, respectively, which were significantly higher than those of the morphine group (P<0.05). For in vitro test, contractions amplitudes of colon muscle strips of the morphine group were (0.568±0.014) g. After being treated with ghrelin of 0.1 μmol/L, 1 μmol/L, and 10 μmol/L, contraction amplitudes increased to (0.624±0.118), (0.673±0.014), and (0.712±0.125) g, respectively, which were significantly higher than those of the morphine group (P<0.05).  Conclusion  Ghrelin can significantly improve the colonic motility of morphine induced bowel dysfunction of mice.

Key words: growth hormone secretagogue receptor agonist, opioid-induced bowel dysfunction, colonic motility