上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

培美曲塞脂质体的制备及其对乳腺癌的作用观察

柏方 1，陈婷 2，刘艳 2，吴克瑾 3，陈青 1，陆云姝 1，葛梅馨 1   

  1. 1. 上海交通大学医学院附属新华医院普外科，上海 200092；2. 上海交通大学医学院附属新华医院药剂科，上海 200092；3. 复旦大学附属妇产科医院乳腺外科，上海 200011
  • 出版日期:2017-02-28 发布日期:2017-02-28
  • 通讯作者: 吴克瑾，电子信箱：kejinwu@163.com。
  • 作者简介:柏方(1990—)，女，硕士生；电子信箱：sme1203@sjtu.edu.cn。
  • 基金资助:

    上海市卫生和计划生育委员会基金（20154Y0077）；上海高校选拔培养优秀青年教师科研专项基金（zzjdyx13087）；上海交通大学医学院附属新华医院基金（14YJ25）

Preparation of liposomal pemetrexed and its effects on breast cancer

BAI Fang1, CHEN Ting2, LIU Yan2, WU Ke-jin3, CHEN Qing1, LU Yun-shu1, GE Mei-xin1   

  1. 1. Department of General Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 2. Department of Pharmacy, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 3. Department of Breast Surgery, Obstetrics and Gynaecology Hospital, Fudan University, Shanghai 200011, China

  • Online:2017-02-28 Published:2017-02-28
  • Supported by:

    Foundation of Shanghai Municipal Health and Family Planning Commission, 20154Y0077; Special Scientific Fund for Cultivation of Outstanding Young
    Teachers in Shanghai Universities, zzjdyx13087; Research Foundation of Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, 14YJ25

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摘要：

目的 ·制备培美曲塞脂质体，观察其对乳腺癌细胞MCF-7及荷瘤裸鼠模型肿瘤的抑制作用。方法 ·采用薄膜分散法制作脂质体，CCK-8法检测其对MCF-7细胞的抑制作用；建立Balb/c裸鼠荷瘤模型，通过尾静脉给药以检测其抑瘤作用。结果 ·培美曲塞脂质体可抑制MCF-7细胞增殖，当培美曲塞浓度分别为0.20、0.40和10.00 μg/mL时，实验组（培美曲塞脂质体）的细胞生存率显著小于对照组（等浓度梯度培美曲塞），P值分别为0.013、0.035和0.041。与空白组（等剂量PBS）比较，实验组（培美曲塞脂质体）和对照组（等剂量培美曲塞）Balb/c裸鼠肿瘤的体积和质量均显著减小；实验组肿瘤的体积和质量也均显著小于对照组（均P=0.000）。结论 ·相对于培美曲塞原料药物，脂质体能抑制MCF-7细胞的增殖，通过尾静脉给药能有效抑制肿瘤在Balb/c裸鼠体内的生长。

关键词: 培美曲塞, 脂质体, 乳腺癌, MCF-7细胞, Balb/c裸鼠

Abstract:

Objective · To prepare the liposomal pemetrexed and investigate its effects on MCF-7 breast cancer cells in vitro and nude mice bearing MCF-7
xenograft tumors. Methods · Liposomal pemetrexed was prepared by film dispersion method. Inhibition of MCF-7 breast cancer cell lines was evaluated
by CCK-8 method, and anti-tumor effects were investigated on Balb/c nude mice bearing MCF-7 xenograft tumors. Results · Liposomal pemetrexed
inhibited the growth of MCF-7 cells. When the concentrations of pemetrexed were 0.20, 0.40 and 10.00 μg/mL, the cell viability in experiment group
(liposomal pemetrexed) was significantly lower than that in control group (pemetrexed of same concentration gradient), with P values of 0.013, 0.035 and
0.041, respectively. Compared with blank group (same volume of PBS), the volumes and weights of tumors of nude mice in experiment group(liposomal
pemetrexed) and control group (same volume of pemetrexed) were significantly lower, and the volume and weight of tumor in experiment group were
also significantly lower than those in control group (P=0.000). Conclusion · Compared to bulk drug of pemetrexed, liposomal pemetrexed can inhibit the
growth of MCF-7 breast cancer cells and the Balb/c nude mice bearing MCF-7 xenograft tumors.

Key words:  pemetrexed, liposome, breast neoplasm, MCF-7 cell, Balb/c nude mouse