上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（临床研究） • 上一篇    下一篇

基于GC-TOFMS技术的缺血性脑卒中代谢组学研究

刘玉敏 1，魏珍玉 2，陈昕 2，王岬琰 2，吴丹红 2   

  1. 1. 上海交通大学分析测试中心，上海 201100；2. 上海交通大学 医学院附属第九人民医院神经内科，上海 201999
  • 出版日期:2017-02-28 发布日期:2017-02-28
  • 通讯作者: 吴丹红，电子信箱：kathywuxue@sina.com。
  • 作者简介:刘玉敏（1970—），女，副研究员，博士；电子信箱：ymliu@sjtu.edu.cn。
  • 基金资助:

    上海交通大学“医工交叉”科研基金（YG2013MS23）；上海市科学技术委员会科研基金（14411972000）；上海交通大学医学院科研基金（152H2013）

Metabonomic study of ischemic stroke based on GC-TOFMS approach

LIU Yu-min1, WEI Zhen-yu2, CHEN Xin2, WANG Jia-yan2, WU Dan-hong2   

  1. 1. Instrumental Analysis Centre, Shanghai Jiao Tong University, Shanghai 201100, China; 2. Department of Neurology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201999, China
  • Online:2017-02-28 Published:2017-02-28
  • Supported by:

    Shanghai Jiao Tong University Fund for Interdisciplinary Research on Medicine and Engineering，YG2013MS23；Science and Technology Commission of Shanghai Foundation，14411972000；Shanghai Jiao Tong University School of Medicine Fund，152H2013

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摘要：

目的 ·研究缺血性脑卒中患者血清代谢物的变化情况及生物学意义。方法 ·采用气相色谱-飞行时间质谱联用（GC-TOFMS）技术检测29名缺血性脑卒中患者和31名正常健康者的血清样本，用多维统计方法分析2组间的差异性代谢物。结果 ·在正交偏最小二乘法分析(OPLS)模型中缺血性脑卒中组与正常健康组的得分图呈现区分。患者血清中一些代谢物的水平发生了显著性的改变：γ 氨基丁酸、戊二酸、甘油酸、葡萄糖酸、乳糖酸和胆固醇等6种代谢物水平呈显著性升高，而柠檬酸、乌头酸、苹果酸、琥珀酸、β-丙氨酸、甘油-3-磷酸等19种代谢物水平呈显著性降低。结论 ·缺血性脑卒中患者体内氨基酸代谢与糖代谢紊乱以及能量相关的
三羧酸循环( TCA)失衡，代谢组学方法可为临床上脑卒中的机制研究提供一条新的途径。

关键词: 缺血性脑卒中, 代谢组学, 气相色谱-飞行时间质谱联用

Abstract:

 Objective · To study the metabolite profiles on patients of ischemic stroke using metabonomics approach. Methods · The serum samples from the 29 patients with ischemic stroke and 31 healthy controls were analyzed by gas chromatography time-of-flight mass spectrometry (GC-TOFMS) coupled multi-dimensional statistical methods to find differential metabolites in two groups. Results · Orthogonal partial least squares analysis (OPLS) model was generated based on identified metabolites and shown clear discrimination from patients and healthy controls. Some serum metabolite levels were significantly altered in patients. Six up-regulated metabolites included γ- aminobutyric acid, glutaric acid, glyceric acid, gluconic acid, lactobionic acid, and cholesterol, and nineteen down-regulated metabolites included citric acid, aconitic acid, malic acid, succinic acid, β-alanine, and glycerol-3 phosphate. Conclusion · Amino acid metabolism, glycometabolism and tricarboxylic acid (TCA) cycle are disturbed in patient of ischemic stroke. The metabonomic approach has great potential to understand the underlying mechanisms of stroke in ischemic patients.

Key words: ischemic stroke, metabonomics, gas chromatography time-of-flight mass spectrometry