上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

Let-7a通过调控骨髓间充质干细胞增殖影响骨质疏松的研究

闫娈，杨德琴   

  1. 重庆医科大学附属口腔医院牙体牙髓科，口腔疾病与生物医学重庆市重点实验室，重庆市高校市级口腔生物医学工程重点实验室，重庆
  • 出版日期:2017-04-28 发布日期:2017-05-04
  • 通讯作者: 杨德琴，电子信箱：2205677883@qq.com。
  • 作者简介:闫娈（1992—）女，住院医师，硕士生；电子信箱：24425122@qq.com。
  • 基金资助:

    国家自然科学基金（31571508）；重庆市卫生局医学科研计划重点项目（2011-1-062）

Effect of Let-7a on osteoporosis by regulating proliferation of bone marrow mesenchymal stem cells

YAN Luan, YANG De-qin   

  1. Department of Endodontics, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Affiliated Hospital of Stomatology, Chongqing Medical University Chongqing 401147, China
  • Online:2017-04-28 Published:2017-05-04
  • Supported by:

    National Natual Science Foundation of China，31571508；Key Research Project of Chongqing Municipal Health Bureau of Health Medical Research Projects，2011-1-062

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摘要：

目的 ·研究雌激素缺乏所致骨质疏松发生过程中 Let-7a对骨髓间充质干细胞 (BMSC)增殖能力的影响。方法 ·取20只8周龄健康雌性小鼠，行双侧卵巢切除术(OVX)，建立雌激素缺乏骨质疏松动物模型。另取20只健康雌性小鼠行卵巢附近脂肪组织部分切除术（sham）。术后2个月，Micro-CT检测2组小鼠股骨干骺端骨密度、骨体积分数及骨小梁数量；MTT检测2组BMSC的增殖能力；real-time PCR检测2组BMSC中Let-7a的表达；上调或下调BMSC内Let-7a的表达水平后，MTT检测BMSC的增殖能力。结果 · Micro-CT显示OVX组股骨干骺端骨密度、骨体积分数及骨小梁数量均低于sham组。成脂能力及成骨能力检测发现，OVX组BMSC成骨能力低于sham组，成脂能力高于sham组。MTT显示OVX组BMSC增殖能力低于sham组。OVX组BMSC中Let-7a的表达明显高于sham组。上调Let-7a的表达水平后，BMSC增殖水平下降；下调Let-7a的表达水平后，BMSC增殖水平上升。结论 ·在雌激素缺乏所致的骨质疏松小鼠中，高表达的Let-7a抑制BMSC增殖，可能是导致骨质疏松发生的原因之一。

关键词: 骨髓间充质干细胞, 增殖, Let-7a

Abstract:

Objective · To explore the effect of Let-7a regulating bone mesenchymal stem cells (BMSC) proliferation on osteoporosis. Methods · Eightweek-old healthy female mice were obtained to establish ovariectomy (OVX) and sham animal models. Micro-CT was applied to detect the parameters of bone density, bone volume fraction and trabecular number of both groups two months after surgery. MTT was used to detect the proliferation ability of BMSC in OVX and sham groups. Real-time PCR was used to detect the expression of Let-7a. After up-regulating or down-regulating the expression of Let-7a, MTT was also used to detect the proliferation ability of BMSC. Results · The results of Micro-CT showed that compared with sham group, bone density, bone volume fraction and trabecular number of OVX group were significant declined. Increased adipogenic ability and impaired osteogenic ability was found in BMSC in OVX group, in comparison with sham group. The proliferation ability of BMSC in OVX group was declined compared with sham group. The expression of Let-7a in OVX group was significantly increased compared with Sham group. The proliferation ability of BMSC was decreased when the expression of Let-7a was up-regulated, while increased when its expression was down-regulated. Conclusion · High expression of Let-7a inhibit the proliferation of BMSC, which may result in the pathogenesis of postmenopausal osteoporosis.

Key words: bone mesenchymal stem cells, proliferation, Let-7a