上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（临床研究） • 上一篇    下一篇

多囊卵巢综合征患者结合珠蛋白基因型及血清水平的研究

王柳，丘彦   

  1. 重庆医科大学附属第一医院生殖健康与不孕症专科，重庆 400016
  • 出版日期:2017-04-28 发布日期:2017-05-04
  • 通讯作者: 丘彦，电子信箱：cqartq@163.com。
  • 作者简介:王柳（1990—），女，硕士生；电子信箱：fulittle@163.com。

Study on haptoglobin polymorphism and plasma concentration in patients with polycystic ovarian syndrome

WANG Liu, QIU Yan   

  1. Department of Assistant Reproductive Centre, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
  • Online:2017-04-28 Published:2017-05-04

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摘要：

目的 ·探讨多囊卵巢综合征（PCOS）患者血清结合珠蛋白（Hp）水平及其与各代谢指标的相关性；探讨PCOS患者Hp基因型和基因频率的分布及其与PCOS之间的关系。方法 ·病例组选取106例PCOS患者，对照组选取86例无高雄激素血症、月经规律的妇女。酶联免疫吸附测定（ELISA）检测血清Hp水平；聚合酶链反应（PCR）检测Hp基因型和基因频率的分布。结果 · PCOS组血清Hp水平显著高于对照组（P=0.000），分析结果表明血清Hp水平变化与体质量指数（BMI）、稳态模型胰岛素抵抗指数（HOMAIR）正相关(均P=0.000)；PCOS组Hp2-2（Hp2 /Hp2）基因型显著高于对照组（P=0.006），Hp2基因频率明显升高（P=0.002）。结论 · PCOS患者血清Hp水平与BMI和HOMA-IR正相关，可能与肥胖和胰岛素抵抗的发生发展有关。携有Hp2-2基因型的人群可能对PCOS有较高的遗传易感性，Hp2等位基因在 PCOS中有聚集现象。

关键词: 多囊卵巢综合征, 结合珠蛋白, 基因型, 基因频率

Abstract:

Objective · To investigate the correlation between the serum haptoglobin (Hp) level and the metabolic parameters in patients with polycystic ovarian syndrome (PCOS), and explore the distribution of Hp genotype and allele frequency in PCOS patients and their correlations with PCOS. Methods · A total of 106 patients with PCOS and 86 cases of patients with non-hyperandrogenic and regular menstruation (control group) were enrolled. Serum Hp level was measured by enzyme-linked immunosorbent assay (ELISA); Hp genotype and allele frequency were determined by polymerase chain reaction (PCR), respectively. Results · The serum Hp level in PCOS group was significant higher than that in the control group (P=0.000). Correlation analysis reveals that serum Hp level was positively related to body mass index (BMI) (P=0.000) and homeostatic model assessment insulin resistance (HOMA-IR) (P=0.000). Compared with control group, proportion of Hp2-2 genotype (Hp2/Hp2) increased significantly in PCOS group (P=0.006), and the frequency of Hp2 allele was also higher (P=0.002). Conclusion · Serum Hp level was positively related to BMI and HOMA-IR, which maybe associated with the occurrence and development of obesity and insulin resistance. Patients with Hp2-2 genotype might have genetic predisposition to PCOS, and high frequency Hp2 allele was found in them.

Key words: polycystic ovarian syndrome, haptoglobin, phenotypes, gene frequency