上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2017, Vol. 37 ›› Issue (8): 1069-.doi: 10.3969/j.issn.1674-8115.2017.08.004

• 论著（基础研究） • 上一篇    下一篇

产超广谱β- 内酰胺酶大肠埃希菌噬菌体的分离及特性研究

黄磊杰1*，刘晓亮1*，朱锐1，林芳琪1，秦金红2，何平2，郭晓奎2，赵蔚1   

  1. 上海交通大学 医学院 1. 实验教学中心，上海200025；2. 免疫学与微生物学系，上海200025
  • 出版日期:2017-08-28 发布日期:2017-09-28
  • 通讯作者: 赵蔚，电子信箱：zhaoweiatsh@163.com
  • 作者简介:黄磊杰（1993—），男，博士生；电子信箱：864620739@qq.com。刘晓亮（1995—），男，博士生；电子信箱：sylar_lxl@outlook.com。* 为共同第一作者。
  • 基金资助:
    上海市公共卫生三年行动计划重点学科建设项目传染病与卫生微生物学（15GWZK0102）

Isolation and characterization of bacteriophage against extended-spectrum β-lactamase-producing Escherichia coli strains

HUANG Lei-jie1*, LIU Xiao-liang1*, ZHU Rui1, LIN Fang-qi1, QIN Jin-hong2, HE Ping2, GUO Xiao-kui2, ZHAO Wei1   

  1. 1. Experiment Teaching Center of Basic Medicine, 2. Department of Microbiology and Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Online:2017-08-28 Published:2017-09-28
  • Supported by:
    Shanghai Three-year Plan of the Key Subjects Construction in Public Health-Infectious Diseases and Pathogenic Microorganism, 15GWZK0102

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摘要： 目的 · 分离并研究裂解产超广谱β- 内酰胺酶（ESBLs）大肠埃希菌的噬菌体，为建立大肠埃希菌噬菌体库及应用噬菌体控 制细菌感染提供研究基础。方法 · 将污水与93 株产ESBLs 的大肠埃希菌共培养，采用双层琼脂平板法分离纯化噬菌体，得到噬菌 体 JDEC001，并进行生物学特性研究及测序和基因组比对分析。结果 · 该噬菌体是一种毒性噬菌体，属于有尾噬菌体目短尾噬菌 体科。在pH 值 5 ～ 11 的范围内及在0 ～ 39 ℃时保持较好的活性。测序结果显示其基因组为双链DNA 分子，全长38 745 bp，GC 含量49.93%，预测有46 个开放阅读框，不编码毒力因子和耐药基因。结论 · 分离纯化得到了一株产ESBLs 大肠埃希菌的噬菌体 JDEC001，在较宽的 pH 和温度范围内稳定性良好。

关键词:  产超广谱 β- 内酰胺酶大肠埃希菌, 噬菌体, 生物学特性, 基因组特性

Abstract:

Objective · To isolate phages which can fight against extended-spectrum β-lactamase (ESBLs)-producing Escherichia coli (E. coli), and provide basic research for establishment of E. coli phage library and treatment of bacterial infection.  Methods · Samples collected from sewage were co-cultured with 93 ESBLs-producing E. coli strains. A phage named JDEC001 was isolated by double agar overlay plaque assay. The biological characteristics, complete genome sequence and comparative genome analyses of JDEC001 were studied respectively.  Results · JDEC001 belongs to the lytic phage as a member of the Caudovirales order, Podoviridae family. It has high activity at pH from 5 to 11 and with temperature from 0 to 39 ℃ . Whole-genome sequencing of JDEC001 demonstrated double-stranded DNA genome of 38 745 bp with GC content of 49.93%, which encoded 46 open reading frames. The comparative genomics also showed that there was no virulent genes or antibiotic resistant genes in its genome.  Conclusion · The phage JDEC001 against ESBLs-producing E. coli was isolated and purified, with good stability in a broad range of pH and temperature.

Key words:  extended-spectrum β-lactamase-producing Escherichia coli, phages, biological characteristics, genomic characteristics