上海交通大学学报(医学版) ›› 2017, Vol. 37 ›› Issue (10): 1402-.doi: 10.3969/j.issn.1674-8115.2017.10.019

• 论著(临床研究) • 上一篇    下一篇

米非司酮对人输卵管上皮纤毛摆动频率及雌、孕激素受体的影响#br#

袁江静 1,赵伟红 1,张朵 2,何晓英 1,张健 1   

  1. 上海交通大学 医学院附属国际和平妇幼保健院  1. 妇科,2. 中心实验室 , 上海 200030
  • 出版日期:2017-10-28 发布日期:2017-11-01
  • 通讯作者: 张健,电子信箱:ipmch@foxmail.com
  • 作者简介:袁江静(1980—),女,主治医师,硕士;电子信箱:drtracy80@sina.com

Effect of mifepristone on ciliary beat frequency and estrogen and progesterone receptor expression levels in human fallopian tubes

YUAN Jiang-jing1, ZHAO Wei-hong1, ZHANG Duo2, HE Xiao-ying1, ZHANG Jian1   

  1. 1. Department of Gynecology, 2. Department of Central Laboratory, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China
  • Online:2017-10-28 Published:2017-11-01

摘要: 目的 · 探讨米非司酮在体外对人输卵管上皮纤毛摆动(CBF)及雌、孕激素受体表达的影响。方法 · 将 25 例人输卵管上皮组 织在体外经米非司酮(0.1、1、10 μmol/L)、孕酮 10 μmol/L、米非司酮(0.1、1、10 μmol/L)+ 孕酮 10 μmol/L 分别培养 24 h 后(另 设空白对照组),测得 CBF 值;应用实时荧光定量 PCR 和免疫组织化学技术分析经米非司酮处理后人输卵管上雌激素受体 α(ERα) 和孕激素受体(PR)的变化;透射电子显微镜观察 10 μmol/L 米非司酮对人输卵管上皮超微结构的影响。结果 · 与空白对照组相比, 米非司酮在0.1、1、10 μmol/L 浓度时对输卵管CBF 无显著影响(P=0.728,P=0.405,P=0.941);孕酮 10 μmol/L 组 CBF 明显下降 (P=0.000),米非司酮在 0.1、1、10 μmol/L 浓度时,呈剂量-反应拮抗孕酮下调 CBF 的效应(P=0.484,P=0.000,P=0.000)。经米非 司酮培养 24 h 后,输卵管上的 ERα、PR 表达上调;输卵管上皮纤毛超微结构与空白对照组比较,无明显差异。结论 · 米非司酮在人 输卵管上发挥孕酮拮抗效应,这可能是这类常用口服紧急避孕药的输卵管避孕机制。

关键词: 米非司酮, 纤毛摆动频率, 人输卵管, 雌激素受体, 孕激素受体

Abstract: Objective · To investigate ciliary beat frequency (CBF), and estrogen and progesterone receptor expression levels of human fallopian tubes after mifepristone treating in vitro.  Methods · Human fallopian tube mucosa explants (n=25) were treated with different concentrations of mifepristone (0.1, 1 and 10 μmol/L) or progesterone (10 μmol/L) separately, or both mifepristone and progesterone. After 24 h of treatment, CBF was measured. Quantitative real-time PCR and immunohistochemistry were used to research the expression of estrogen receptor-α (ERα) and progesterone receptor (PR) of human fallopian tubes after mifepristone treating. The ultrastructure of epithelial cells of fallopian tube after mifepristone (10 μmol/L) treating were observed with transmission electron microscopy.  Results · The CBF at the concentrations of 0.1, 1 and 10 μmol/L was not affected by mifepristone (P=0.728, P=0.405 and P=0.941). The CBF decreased markedly in the group of 10 μmol/L progesterone compared to control group (P=0.000). Mifepristone (0.1, 1 and 10 μmol/L) dose dependently antagonized the progesterone-induced CBF decrease (P=0.484, P=0.000 and P=0.000). Mifepristone upregulated the expression levels of ERα and PR in the fallopian tubes, but the ultrastructure of the cilia had no significant change.  Conclusion · Mifepristone acts as progesterone antagonist in the human fallopian tube, which may explain the tubal contraceptive mechanism when used as an emergency contraceptive.

Key words: mifepristone, ciliary beat frequency, human fallopian tube, estrogen receptor, progesterone receptor