上海交通大学学报(医学版) ›› 2018, Vol. 38 ›› Issue (11): 1289-.doi: 10.3969/j.issn.1674-8115.2018.11.003

• 论著·基础研究 • 上一篇    下一篇

表没食子儿茶素没食子酸酯对3T3-L1脂肪细胞氧化应激和炎症水平的影响

买地娜依 ·库得来提 1,唐文静 2,何雯楠 2,汪佳璐 2,宋还雷 3,沈秀华 2, 3   

  1. 1.上海交通大学公共卫生学院,上海 200025;2.上海交通大学医学院营养系,上海 200025;3.上海交通大学医学院附属新华医院临床营养科,上海 200092
  • 出版日期:2018-11-28 发布日期:2018-12-15
  • 通讯作者: 沈秀华,电子信箱:srachel@126.com。
  • 作者简介:买地娜依 ·库得来提 (1989— ),女,维吾尔族,硕士生;电子信箱: madina610@126.com。
  • 基金资助:
    国家自然科学基金(81773407,81102123)

Effect of epigallocatechin-3-gallate on oxidative stress and inflammation in 3T3-L1 adipocytes

Maidinayi · KUDELAITI1, TANG Wen-jing2, HE Wen-nan2, WANG Jia-lu2, SONG Huan-lei3, SHEN Xiu-hua2, 3   

  1. 1. Shanghai Jiao Tong University School of Public Health, Shanghai 200025, China; 2. Faculty of Nutrition, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 3. Department of Clinical Nutrition, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
  • Online:2018-11-28 Published:2018-12-15
  • Supported by:
    National Natural Science Foundation of China,81773407,81102123

摘要: 目的 ·初步探索表没食子儿茶素没食子酸酯( epigallocatechin-3-gallate,EGCG)对 3T3-L1脂肪细胞氧化应激和炎症水平的影响,为 EGCG防治肥胖及其相关慢性病提供理论依据。方法 ·采用体外细胞培养法,将 3T3-L1前脂肪细胞诱导分化为成熟 3T3L1脂肪细胞。实验分组为空白对照组, 1 μg/mL、10 μg/mL、50 μg/mL EGCG组,干预 24 h,检测细胞内氧化应激指标谷胱甘肽(glutathione,GSH)、超氧化物歧化酶( superoxide dismutase,SOD)、丙二醛( malondialdehyde,MDA)含量;采用 ELISA法、实时荧光定量 PCR法分别检测炎症指标白细胞介素 -6(interleukin-6,IL-6)、肿瘤坏死因子 -α(tumor necrosis factor-α,TNF-α)、单核细胞趋化蛋白 -1(monocyte chemoattractant protein-1,MCP-1)含量和 mRNA水平,采用实时荧光定量 PCR法检测核因子 E2相关因子 2(nuclear factor E2-related factor 2,Nrf2)、血红素氧合酶 -1(heme oxygenase-1,HO-1)表达量。结果 · EGCG各干预组 3T3-L1脂肪细胞内 GSH、SOD含量均较空白对照组升高(均 P<0.05),MDA含量则均较空白对照组降低(均 P<0.05),并呈剂量依赖性。经 ELISA法检测发现 1 μg/mL、10 μg/mL、50 μg/mL EGCG干预后 3T3-L1脂肪细胞上清液中 IL-6、MCP-1和 TNF-α的含量均较对照组显著下降(均 P<0.05),并呈剂量依赖性;经实时荧光定量 PCR法检测发现不同浓度 EGCG干预 24 h后,3T3-L1脂肪细胞内 IL-6、 MCP-1、TNF-α mRNA表达量均较对照组逐渐下降,呈剂量依赖性;经 10 μg/mL、50 μg/mL EGCG干预 24 h后 Nrf2、HO-1 mRNA表达量均较对照组显著上升(均 P<0.05)。结论 · EGCG可以抑制 3T3-L1脂肪细胞的氧化应激和炎症水平,此作用可能与上调 Nrf2/ HO-1有关。

关键词: 表没食子儿茶素没食子酸酯(EGCG), 肥胖, 脂肪细胞, 氧化应激, 炎症

Abstract: Objective · To explore the effect of epigallocatechin-3-gallate (EGCG) on oxidative stress and inflammation in 3T3-L1 adipocytes, and provide a theoretical basis for EGCG to prevent obesity and related chronic diseases. Methods · 3T3-L1 preadipocytes were differentiated to mature adipocytesin vitro cell culture. The cells were divided into blank control group, and 1, 10 and 50 μg/mL EGCG groups. After 24 hour treatment, intracellular oxidative stress indicators glutathione (GSH), superoxide dismutase (SOD), and malondialdehyde (MDA) levels in the cells were measured. The levels of inflammatory indexes interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and monocyte chemoattractant protein-1 (MCP-1) were testedELISA and realtime PCR, while the of nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) was testedrealtime PCR. Results · Compared with the control group, GSH and SOD levels in 3T3-L1 adipocytes increased in a dose-dependent manner after treatment of EGCG (both P<0.05), while MDA level in 3T3-L1 adipocytes decreased dose-dependently after treatment of EGCG (P<0.05). IL-6, MCP-1 and TNF-α levels in 3T3L1 adipocytes supernatant declined significantly in a dose-dependent manner after treatment of 1, 10 and 50 μg/mL EGCG (all P<0.05). The levels of IL-6, MCP-1 and TNF-α in 3T3-L1 adipocytes were decreased in a dose-dependent manner after 24 h treatment of different concentrations of EGCG. Nrf2 and HO-1 mRNA levels in 3T3-L1 adipocytes increased significantly in a dose-dependent manner after treatment of 10 and 50 μg/mL EGCG (both P<0.05). Conclusion · EGCG plays an antioxidation and anti-inflammatory effects in 3T3-L1 adipocytes, which may be related to up-regulation of Nrf2/HO-1.

Key words: epigallocatechin-3-gallate (EGCG), obesity, adipocytes, oxidative stress, inflammation

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