上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2019, Vol. 39 ›› Issue (2): 120-.doi: 10.3969/j.issn.1674-8115.2019.02.003

• 论著·基础研究 • 上一篇    下一篇

原花青素 B2对人牙周膜细胞炎症介质表达的影响

顾丽芬 1，杨涛 2，高颖 3，周薇 1，宋忠臣 1   

  1. 上海交通大学医学院附属第九人民医院 ·口腔医学院牙体牙髓科，国家口腔疾病临床医学研究中心，上海市口腔医学重点实验室，上海市口腔医学研究所，上海 200011
  • 出版日期:2019-02-28 发布日期:2019-03-19
  • 通讯作者: 宋忠臣，电子信箱：szhongchen@sina.com。
  • 作者简介:顾丽芬（1980—），女，主治医师，硕士生；电子信箱： 906731251@qq.com。
  • 基金资助:
    国家重点研发计划（2017YFC0840100，2017YFC0840110）

Effect of procyanidin B2 on inflammatory mediators of human periodontal ligament cells

GU Li-fen1, YANG Tao2, GAO Ying3, ZHOU Wei1, SONG Zhong-chen1   

  1. 1. Department of Periodontology, Shanghai Ninth Peoples Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai 200011, China; 2. The 93313 Army of the Chinese People’s Liberation Army, Changchun 130111, China; 3. The 461 Clinical Department of the 208 Hospital of the Chinese Peoples Liberation Army, Changchun 130021, China
  • Online:2019-02-28 Published:2019-03-19
  • Supported by:
    National Key R&D Program of China, 2017YFC0840100，2017YFC0840110

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摘要： 目的 ·观察原花青素 B2对牙龈卟啉单胞菌脂多糖（ lipopolysaccharide，LPS）诱导的人牙周膜细胞（ human periodontal ligament cells，hPDLCs）炎症介质表达的影响。方法 ·组织块法体外培养 hPDLCs；噻唑蓝比色法观察原花青素 B2对 hPDLCs活性的影响；原花青素 B2预处理 hPDLCs 1 h后，予以 LPS，实时荧光定量 PCR和 ELISA检测炎症因子白细胞介素 1β（IL-1β）、 IL-6及 IL-8的 mRNA和蛋白的表达；荧光显微镜下观察细胞内活性氧的变化； Griess法检测培养上清液中一氧化氮（ NO）的水平。结果 ·当原花青素 B2浓度达到 100.00 μg/mL时可增强 hPDLCs的活性。原花青素 B2可抑制 LPS诱导的 IL-1β、IL-6及 IL-8的 mRNA和蛋白的表达，降低活性氧和 NO的水平。结论 ·原花青素 B2可抑制牙龈卟啉单胞菌 LPS诱导的 hPDLCs炎症介质的表达，发挥一定的抗炎作用。

关键词: 牙周膜细胞, 原花青素 B2, 脂多糖, 炎症介质

Abstract:

Objective · To investigate the effects of procyanidin B2 on the of inflammatory mediators in human periodontal ligament cells (hPDLCs) inducedPorphyromonas gingivalis lipopolysaccharide (LPS). Methods · hPDLCs were cultured using tissue explant method in vitro. The effect of procyanidin B2 on the cell viability of hPDLCs was detectedMTT assay. hPDLCs were stimulatedP. gingivalis LPS after treatment with procyanidin B2 for 1 h. The s of IL-1β, IL-6, and IL-8 mRNA and proteins were detectedreal-time PCR and ELISA assay. Reactive oxygen species (ROS) in the cytoplasm was observed under fluorescence microscope. Nitric oxide (NO) in the supernatant was detectedGriess assay. Results · 100.00 μg/mL procyanidin B2 could enhance the cell viability of hPDLCs. Procyanidin B2 could inhibit the s of IL-1β, IL-6, and IL-8 mRNA and proteins in hPDLCs. It could also downregulate ROS and NO in hPDLCs inducedP. gingivalis LPS. Conclusion · Procyanidin B2 can play an anti-inflammatory roleinhibiting inflammatory mediators in hPDLCs inducedP. gingivalis LPS.

Key words: periodontal ligament cell, procyanidin B2, lipopolysaccharide, inflammatory mediator

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