上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2019, Vol. 39 ›› Issue (5): 487-.doi: 10.3969/j.issn.1674-8115.2019.05.008

• 论著·基础研究 • 上一篇    下一篇

利拉鲁肽对变应性鼻炎小鼠模型的治疗作用

孔德弟，王俊，向明亮，孙星梅，王士礼   

  1. 上海交通大学医学院附属瑞金医院耳鼻咽喉科，上海 200025
  • 出版日期:2019-05-28 发布日期:2019-07-26
  • 通讯作者: 王士礼，电子信箱：shiliwang101@126.com。
  • 作者简介:孔德弟（1991—），男，硕士生；电子信箱： dedikong@163.com。
  • 基金资助:
    上海市卫生和计划生育委员会科研课题（201540387）

Therapeutical effect of liraglutide treatment in a momodel of allergic rhinitis

KONG De-di, WANG Jun, XIANG Ming-liang, SUN Xing-mei, WANG Shi-li   

  1. Department of Otolaryngology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Online:2019-05-28 Published:2019-07-26
  • Supported by:
    Research Project of Shanghai Health and Family Planning Commission, 201540387

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摘要： 目的 ·探究利拉鲁肽腹腔注射能否改善小鼠变应性鼻炎（ allergic rhinitis，AR）的炎症状态，为 AR提供新的治疗选择。方法 ·选取 SPF级 BALB/c小鼠 18只，采用随机数字表法分为正常对照组、 AR组、治疗组。利用卵清蛋白（ ovalbumin，OVA）和氢氧化铝建立 AR小鼠模型（包括 AR组和治疗组，正常对照组用生理盐水代替），治疗组行利拉鲁肽腹腔注射（另 2组用生理盐水代替）。观察小鼠在利拉鲁肽干预后打喷嚏和挠鼻次数的改变，检测小鼠鼻黏膜内嗜酸性粒细胞浸润数、杯状细胞增生情况以及黏膜形态的变化。酶联免疫吸附实验分别检测小鼠血清中 OVA-specific IgE（OVA-sIgE）、IL-4和 IL-5的浓度。结果 ·对照组鼻黏膜嗜酸性粒细胞浸润少见，偶见杯状细胞增生； AR组杯状细胞增生明显，大量嗜酸性粒细胞浸润，黏膜增厚。 AR组杯状细胞增生、嗜酸性粒细胞数和黏膜厚度较对照组显著增加（均 P<0.05），AR组小鼠的挠鼻及喷嚏数均显著高于对照组（均 P<0.05），而治疗组小鼠上述症状较 AR组均减轻（均 P<0.05）。AR组小鼠血清中 OVA-sIgE、IL-4和 IL-5的浓度显著高于对照组（均 P<0.05），治疗组以上指标均低于 AR组（均 P<0.05）。结论 ·利拉鲁肽腹腔注射可以有效改善小鼠 AR的症状和炎症程度，有可能成为治疗 AR新的研究方向。

关键词: 小鼠模型, 变应性鼻炎, 嗜酸性粒细胞, 胰高血糖素样肽 -1

Abstract:

Objective · To investigate whether intraperitoneal injection of liraglutide can improve the inflammatory state of allergic rhinitis (AR) in mice and provide new treatment options for AR. Methods · Eighteen BALB/c mice (SPF grade) were divided into three groups (control group, AR group and treatment group)random number method. The AR and treatment group were established through intraperitoneal injection of OVA and aluminum hydroxide (replacedphysiological saline in control group). And treatment group received intraperitoneal injection of liraglutide (replacedphysiological saline in control and AR groups). The changes in the numbers of sneezing and nose scratching of mice were observed after the intervention of liraglutide. The number of eosinophil infiltration, goblet cell proliferation and mucosal thickness in mice nasal mucosa were measured. Enzyme-linked immunosorbent assays were used to detect the concentrations of OVA-specific IgE (OVA-sIgE), IL-4 and IL-5 in serum of mice. Results · In control group, there were rare eosinophils and goblet cell hyperplasia. In AR group, obvious goblet cell hyperplasia, significant eosinophil infiltration and thickening mucosal were observed. The number of eosinophil, goblet cell hyperplasia, and mucosal thickness in AR group significantly increased compared with those in control group (all P<0.05). The numbers of nose scratching and sneezing in AR group were significantly higher than those in the control group (both P<0.05), and the above symptoms in treatment group were improved compared with AR group (both P<0.05). The serum concentrations of OVA-sIgE, IL-4 and IL-5 in AR group were significantly higher than those in control group (all P<0.05), and the above indicators in treatment group were lower than those in AR group (all P<0.05). Conclusion · Intraperitoneal injection of liraglutide can effectively improve the symptoms and inflammatory level of AR in mice, which may be a novel research direction in the treatment of AR.

Key words: momodel, allergic rhinitis, eosinophil, glucagon-like peptide-1

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