上海交通大学学报(医学版) ›› 2019, Vol. 39 ›› Issue (8): 820-.doi: 10.3969/j.issn.1674-8115.2019.08.003

• 论著·基础研究 • 上一篇    下一篇

葡萄糖对小鼠 CD4 + T细胞分化的影响和机制初探

陈冬平,伍宁波,苏冰,钮晓音   

  1. 上海交通大学医学院,上海市免疫学研究所,上海 200025
  • 出版日期:2019-08-28 发布日期:2019-09-23
  • 通讯作者: 钮晓音,电子信箱:niuxiaoyin@163.com。
  • 作者简介:陈冬平(1993—),女,硕士生;电子信箱: chenduoduo77@163.com。
  • 基金资助:
    国家自然科学基金(81871269);上海市卫生和计划生育委员会科研课题面上项目(201640137)

Primary study on the effect of glucose on moCD4+ T cell differentiation and its mechanism

CHEN Dong-ping, WU Ning-bo, SU Bing, NIU Xiao-yin   

  1. Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Online:2019-08-28 Published:2019-09-23
  • Supported by:
    National Natural Science Foundation of China, 81871269; Scientific Research Project of Shanghai Municipal Commission of Health and Family Planning, 201640137

摘要: 目的 ·探讨葡萄糖对小鼠 CD4+ T细胞分化的影响。方法 ·小鼠初始 CD4+ T细胞在调节性 T细胞( regulatory T cell,Treg)、 Th1、Th17和 Th2分化条件下,经不同浓度的葡萄糖处理 5 d后,利用流式细胞术检测细胞分化的比例,实时荧光定量 PCR检测相关细胞因子和转录因子的基因表达水平。结果 ·与无糖组相比,在 Treg和 Th2分化的条件下,随着葡萄糖浓度升高, Treg和 Th2比例增高,其关键转录因子 Foxp3(forkhead box P3)、Gata3(GATA binding protein 3)与主要细胞因子转化生长因子 -β、白介素 -4(interleukin-4,IL-4)和 IL-13的基因水平增加;而随着葡萄糖浓度升高, Th1和 Th17细胞比例则降低,其相关转录因子 Tbx21(T-box transcription factor 21)和 RORC(RAR related orphan receptor C),以及细胞因子干扰素 -γ、IL-17A、IL-17F、IL-22和细胞因子受体 IL-23R的基因表达下降。结论 ·葡萄糖能促进 Treg和 Th2体外分化,而抑制 Th1与 Th17分化。

关键词: 葡萄糖, CD4+ T细胞, 调节性 T细胞, Th1细胞, Th17细胞, Th2细胞, 体外分化

Abstract: Objective · To study the effect of glucose on moCD4+ T cell differentiation. Methods · Mona.ve CD4+ T cells cultured in the regulatory T cell (Treg), Th1, Th17 or Th2 differention condition were treated with different concentrations of glucose for 5 days. Treg, Th1, Th17 or Th2 percentages were measuredflow cytometry. Quantitative real-time PCR was used to detect the gene s of related cytokines and transcriptional factors. Results · The proportions of Treg and Th2 as well as the gene s of transforming growth factor-β, interleukin-4 (IL-4) and IL-13, and transcriptional factors, Foxp3 (forkhead box P3) and Gata3 (GATA binding protein 3), were increased significantly with the treatment of increasing concentration of glucose. On the contrary, with the glucose treatment, the percentages of Th1 and Th17 were reduced, and the gene s of the related cytokines and cytokine receptors, such as interferon-γ, IL-17A, IL-17F, IL-22 and IL-23R, and the related transcriptional factors, Tbx21 (T-box transcription factor 21) and RORC (RAR related orphan receptor C), were decreased consistently. Conclusion · Glucose promotes Treg and Th2 differentiation while inhibits Th1 and Th17 differentiation in vitro.

Key words: glucose, CD4+ T cell, regulatory T cell (Treg), Th1 cell, Th17 cell, Th2 cell, in-vitro differentiation

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