上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2020, Vol. 40 ›› Issue (2): 188-.doi: 10.3969/j.issn.1674-8115.2020.02.007

• 论著·基础研究 • 上一篇    下一篇

下调miR-27a表达对三阴乳腺癌细胞增殖、迁移和侵袭的影响

程 龙1，徐五琴2，张 鹏1，黄建军1，李小宁1   

  1. 1. 皖南医学院弋矶山医院检验科，芜湖 241001；2. 皖南医学院弋矶山医院病理科，芜湖 241001
  • 出版日期:2020-02-28 发布日期:2020-03-20
  • 通讯作者: 李小宁，电子信箱：lixiaoning19702006@126.com。
  • 作者简介:程 龙（1985—），男，主管检验师，硕士；电子信箱：chenglong@wnmc.edu.cn。
  • 基金资助:
    重大疾病非编码RNA转化研究安徽普通高校重点实验室（皖南医学院）开放课题（RNA201904）；活性生物大分子研究安徽省重点实验室自主研究项目（LAB201809）。

Effect of miR-27a down-regulation on proliferation, migration and invasion in triple-negative breast cancer cells

CHENG Long, XU Wu-qin, ZHANG Peng, HUANG Jian-jun, LI Xiao-ning   

  1. 1. Clinical Laboratory, Yijishan Hospital of Wannan Medical College, Wuhu 241001, China; 2. Department of Pathology, Yijishan Hospital of Wannan Medical College , Wuhu 241001, China
  • Online:2020-02-28 Published:2020-03-20
  • Supported by:
    Open Project of Key Laboratory for Non-coding RNA Transformation Research of Anhui Higher Education Institution (Wannan Medical College) (RNA201904); Anhui Province Key Laboratory of Active Biological Macro-molecules Research (LAB201809).

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摘要： 目的·探讨下调miR-27a表达对三阴乳腺癌(triple-negative breast cancer，TNBC)细胞系MDA-MB-453增殖、迁移和侵袭的影响。方法·采用实时荧光定量PCR方法检测68例TNBC患者癌组织及癌旁组织标本的miR-27a表达，分析miR-27a表达与患者临床特征的关系；并检测TNBC细胞系MDA-MB-453及正常乳腺上皮细胞MCF-10A中miR-27a表达的差异。在MDA-MB-453细胞中转染miR-27a抑制物（miR-27a inhibitor），采用CCK-8实验、Transwell小室法检测miR-27a inhibitor转染组（miR-27a inhibitor组）与miR-27a阴性对照片段转染组（NC组）TNBC细胞增殖、迁移和侵袭能力的差异。蛋白质印迹法检测转染miR-27a抑制物对MDA-MB-453细胞泛素连接酶FBW7（F-box and WD repeat domain-containing 7）及Egl 9同源物2（Egl nine homolog 2，EGLN2）蛋白表达的影响。结果·TNBC组织中miR-27a表达明显高于癌旁组织，差异有统计学意义（PPPPPP结论·miR-27a在TNBC组织及细胞株中表达升高；下调miR-27a表达可以抑制TNBC细胞MDA-MB-453的增殖、迁移和侵袭能力，这可能与其下调后FBW7蛋白表达增加、EGLN2蛋白表达减少有关。

关键词: miR-27a, 三阴乳腺癌, FBW7, Egl 9同源物2, 增殖, 迁移, 侵袭

Abstract:

Objective · To investigate the effect of miR-27a down-regulation on proliferation, migration and invasion in triple-negative breast cancer (TNBC) cell line MDA-MB-453. Methods · Sixty-eight pairs of breast cancer tissues and para-carcinoma tissues the TNBC cancer patients were harvested for the study. The differential of miR-27a in the collected tissues, MDA-MB-453 cell line and normal breast epithetial cell line MCF-10A were detectedquantitative real-time PCR (qPCR). The relationship between miR-27a and the clinical characteristics of TNBC patients was analyzed. miR-27a inhibitor was transfected into MDA-MB-453 cell line. CCK-8 assay and Transwell chamber test were used to detect the difference in proliferation, migration and invasion of MDA-MB-453 cells between miR-27a inhibitor group and negative control group (NC group). The levels of F-box and WD repeat domain-containing 7 (FBW7) and Egl nine homolog 2 (EGLN2) protein were examinedWestern blotting. Results · The of miR-27a in TNBC tissues was significantly higher than that in para-carcinoma tissues (PPPPPPPConclusion · The of miR-27a in TNBC tissues and TNBC cell line increases. Down-regulation of miR-27a can inhibit the proliferation, migration and invasion of MDA-MB-453 cells, which may be through increasing FBW7 and decreasing EGLN2 .

Key words: miR-27a, triple-negative breast cancer (TNBC), F-box and WD repeat domain-containing 7 (FBW7), Egl nine homolog 2 (EGLN2), proliferation, migration, invasion