上海交通大学学报(医学版) ›› 2020, Vol. 40 ›› Issue (06): 768-775.doi: 10.3969/j.issn.1674-8115.2020.06.010

• 论著·基础研究 • 上一篇    

子宫内膜癌中长链非编码RNA表达谱的生物信息学分析

建方方1,车晓霞2,冯炜炜1   

  1. 1. 上海交通大学医学院附属瑞金医院妇产科,上海 200025;2. 复旦大学附属妇产科医院妇科,上海 200011
  • 出版日期:2020-06-28 发布日期:2020-08-11
  • 通讯作者: 冯炜炜,电子信箱:fww12066@rjh.com.cn。
  • 作者简介:建方方(1987—),女,住院医师,博士;电子信箱:jff12557@rjh.com.cn。
  • 基金资助:
    上海市教育委员会高峰高原学科建设计划(20172003);国家自然科学基金(81700692)。

Bioinformatics analysis of expression profiles of long noncoding RNA in endometrial cancer

JIAN Fang-fang1, CHE Xiao-xia2, FENG Wei-wei1   

  1. 1. Department of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 2. Department of Gynecology, Obstetrics & Gynecology Hospital of Fudan University, Shanghai 200011, China
  • Online:2020-06-28 Published:2020-08-11
  • Supported by:
    Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Grant Support (20172003); National Natural Science Foundation of China (81700692).

摘要: 目的·分析子宫内膜癌组织和正常内膜组织中长链非编码RNA(long noncoding RNA,lncRNA)的差异表达谱。方法·分别抽提21例子宫内膜癌组织和5例正常内膜组织的RNA,利用转录组测序技术分析并筛选2组差异表达lncRNAs。对获得的差异表达lncRNAs进行GO(Gene Ontology)功能分析和KEGG(Kyoto Encyclopedia of Genes and Genomes)通路分析,并结合TCGA数据库对差异表达lncRNAs进行交集分析。结果·共筛选获得3 060个差异表达lncRNAs,其中上调表达的有2 046个,下调的有1 014个。GO功能分析和KEGG通路分析结果显示,这些lncRNAs与细胞黏附、免疫反应、炎症反应、细胞增殖相关,并且主要富集在PI3K-Akt信号通路、细胞黏附、细胞因子受体等通路上。交集分析显示,57个lncRNAs在测序结果和TCGA数据库中同时上调或下调。结论·lncRNAs在子宫内膜癌和正常内膜组织中的表达有显著差异,提示其可能在子宫内膜癌的发生及发展中起到了重要作用。

关键词: 子宫内膜癌, 长链非编码RNA, 基因表达谱

Abstract: Objective · To analyze the differentially expressed profiles of long noncoding RNA (lncRNA) in endometrial cancer (EC) tissues and normal endometrial tissues. Methods · The RNA was extracted from 21 EC tissues and 5 normal endometrial tissues, respectively, and lncRNAs expression profiles were analyzed and screened by transcriptome sequencing technology. Gene Ontology (GO) function analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were carried out for the differentially expressed lncRNAs, and their expression differences between the transcriptome sequencing and TCGA database were analyzed. Results · There were 3 060 differentially expressed lncRNAs, of which 2 046 were up-regulated and 1 014 were down-regulated. GO functional analysis showed that these lncRNAs were associated with cell adhesion, immune response, inflammatory response and cell proliferation. KEGG pathway analysis showed that these lncRNAs were mainly enriched on the pathways, such as PI3K-Akt signaling pathway, cell adhesion and cytokine-cytokine receptor interaction. Intersection analysis showed that 57 lncRNAs were up-regulated or down-regulated simultaneously in the sequencing results and TCGA database. Conclusion · The expression of lncRNAs in EC tissues and normal endometrial tissues are significantly different, suggesting that it may play an important role in the occurrence and development of EC.

Key words: endometrial cancer (EC), long noncoding RNA (lncRNA), gene expression profile

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