上海交通大学学报（医学版）

›› 2009, Vol. 29 ›› Issue (12): 1428-.

• 论著（基础研究） • 上一篇    下一篇

骨髓间充质干细胞对小鼠Lewis肺癌皮下移植瘤的作用

刘 峰, 姜 斌, 张文颖, 王美玲, 袁海花, 胡晓华, 王炯轶, 龚玉芳, 龚圣济   

  1. 上海交通大学 医学院第三人民医院肿瘤科, 上海 201900
  • 出版日期:2009-12-25 发布日期:2009-12-25
  • 通讯作者: 姜 斌, 电子信箱: dr_jiang@yeah.net。
  • 作者简介:刘 峰(1974—), 男, 主治医师, 硕士；电子信箱: nuanliu@126.com。
  • 基金资助:

    上海市教委基金(05BZ04, 08YZ47)和上海交通大学医学院附属第三人民医院基金(syz07-03)

Effect of mesenchymal stem cells on subcutaneous xenograft tumors in mice with Lewis lung cancer

LIU Feng, JIANG Bin, ZHANG Wen-ying, WANG Mei-ling, YUAN Hai-hua, HU Xiao-hua, WANG Jiong-yi, GONG Yu-fang, GONG Sheng-ji   

  1. Department of Oncology, The Third People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 201900, China
  • Online:2009-12-25 Published:2009-12-25
  • Supported by:

    Shanghai Education Committee Foundation, 05BZ04, 08YZ47；Foundation from The Third People's Hospital, School of Medicine, Shanghai Jiaotong University, syz07-03

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摘要：

目的 研究骨髓间充质干细胞(MSCs)对小鼠Lewis肺癌皮下移植瘤的影响。方法 自C57BL/6小鼠骨髓分离MSCs，制备单细胞悬液并于体外传代培养，取第4～5代细胞用于实验。56只C57BL/6小鼠经Lewis肺癌细胞皮下接种建立小鼠肺癌皮下移植瘤模型，根据MSCs给予时间分为D0组(接种同时给予MSCs)和D10组(接种后第10天给予MSCs），其中D0组分为3个亚组（n=8）：组1单纯接种肿瘤细胞，组2肿瘤细胞和MSCs共同接种，组3肿瘤细胞接种及尾静脉注射MSCs；D10组分为4个亚组（n=8）：组4(肿瘤细胞接种及瘤体内注射MSCs)及其等量PBS对照组（组5），组6（肿瘤细胞接种及尾静脉注射MSCs）及其等量PBS对照组（组7）。观察各组移植肿瘤的生长情况，包括肿瘤形成时间及不同时间点的瘤体大小，并进行组间分析比较。结果 在D0组，组1、2、3肿瘤形成时间分别为（9.37±1.41）d、（7.62±1.40）d和（9.25±1.49）d，与组1和组3比较，组2的肿瘤形成时间明显缩短（P<0.05）；而各时间点三组瘤体大小比较，差异无统计学意义（P>0.05）。组4的瘤体显著大于其对照组（P<0.05）；而组6与其对照组的瘤体大小比较，差异无统计学意义（P>0.05）。结论 MSCs与Lewis细胞同时接种可加速小鼠肺癌皮下移植瘤形成，而成瘤后MSCs瘤体内注射具有促进移植瘤生长的作用。

关键词: 间充质干细胞, 肺癌, 肿瘤移植, 小鼠

Abstract:

Objective To investigate the effect of mesenchymal stem cells (MSCs) on subcutaneous xenograft tumors in mice with Lewis lung cancer. Methods MSCs isolated from bone marrow of C57BL/6 mice were made into single cell suspension and were cultured in vitro. The cells of the 4th to 5th passage were used for the subsequent experiments. Fifty six C57BL/6 mice were inoculated subcutaneously with Lewis lung cancer cells, and  were grouped into Group D0 (MSCs were given simultaneously with inoculation)and Group D10(MSCs were given 10 d after inoculation). Group D0 included three subgroups （n=8）: Group 1 with inoculation of tumor cells, Group 2 with inoculation of tumor cells and MSCs, and Group 3 with inoculation of tumor cells and tail intravenous injection of MSCs. Group D10 included four groups: Group 4 with inoculation of tumor cells and injection of MSCs in tumors, Group 5 with equivalent PBS （the control of Group 4）, Group 6 with inoculation of tumor cells and tail intravenous injection of MSCs, and Group 7 with equivalent PBS （the control of Group 6）. The time of tumor formation and the volume of tumor were observed and compared among the groups. Results In Group D0, earlier onset of tumor development was observed in Group 2 as compared to Group 1 and Group 3 （P<0.05）, while there was no significant difference on the volume of tumor in the three groups (P>0.05). In Group D10, the volume of tumors were larger in Group 4 compared to the control （P<0.05）, while there was no significant difference on the volume of tumors between Group 6 and the control （P>0.05）. Conclusion Inoculating mixture of MSCs and Lewis lung cancer cells  accelerates tumor formation,and injection of MSCs in tumors stimulates the growth of tumors.

Key words: mesenchymal stem cells, lung cancer, transplantation of tumor, mouse