上海交通大学学报（医学版）

›› 2009, Vol. 29 ›› Issue (12): 1455-.

• 论著（基础研究） • 上一篇    下一篇

康莱特注射液对Lewis肺癌小鼠免疫功能的影响

吴 岩, 潘 沛, 王彧杰, 王 蓉, 原永芳   

  1. 上海交通大学 医学院第三人民医院药剂科, 上海 201900
  • 出版日期:2009-12-25 发布日期:2009-12-25
  • 通讯作者: 原永芳, 电子信箱: nmxyyf@yahoo.cn。
  • 作者简介:吴 岩（1983—）, 女, 硕士生；电子信箱: fs-wu-yan@163.com。
  • 基金资助:

    上海市教委基金（063B2044）

Effect of Kanglaite injection on immune function of rats with Lewis lung carcinoma

WU Yan, PAN Pei, WANG Yu-jie, WANG Rong, YUAN Yong-fang   

  1. Department of Pharmacy, The Third People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 201900, China
  • Online:2009-12-25 Published:2009-12-25
  • Supported by:

    Shanghai Education Committee Foundation, 063B2044

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摘要：

目的 观察康莱特注射液（KLT）对Lewis肺癌小鼠模型免疫功能的影响。方法 将40只C57BL/6小鼠制成Lewis肺癌模型，随机分为模型组和KLT高、中、低剂量组，每组10只。建模第2天起，KLT高、中、低剂量组每天分别给予腹腔注射KLT 25、12.5、6.25 mL/kg，连续14 d；模型组不予任何处理。第16天，处死小鼠，称取体质量、肿瘤和脾脏质量，计算肿瘤抑制率和脾脏指数；MTT法检测脾脏中T细胞增殖活力；ELISA检测脾细胞中IL-2的表达；Western blot检测脾脏组织中NF-κB和IκBα蛋白的表达。结果 KLT高、中、低剂量组的肿瘤抑制率逐渐降低；KLT高、中剂量组的脾脏指数高于模型组（P<0.05或P<0.01）。与模型组比较，KLT高、中、低剂量组脾脏中T细胞增殖活力明显升高，KLT高、中剂量组脾细胞中IL-2的表达水平明显升高（P<0.05或P<0.01）。与模型组比较，KLT高、中、低剂量组胞核中NF-κB蛋白表达随给药剂量增加而升高；胞质中NF-κB和IκBα蛋白表达随给药剂量增加而下降。结论 KLT在抗肿瘤的同时能通过提高T细胞增殖活力，调节IL-2、NF-κB、IκBα等因子的表达，达到增强机体免疫功能的目的。

关键词: 康莱特注射液, Lewis肺癌, 免疫功能, 小鼠

Abstract:

Objective To investigate the effect of Kanglaite injection (KLT) on immunological function of rat models with Lewis lung carcinoma. Methods Forty C57BL/6 mice were used to establish Lewis lung carcinoma models and divided randomly into the high dose(25 mL/kg), middle dose (12.5 mL/kg) and low dose (6.25 mL/kg) of KLT groups and model group（n=10）. The mice in the KLT groups were sacrificed after injecting corresponding dose of KLT with intraperitoneal injection for 14 d. No treatment was performed on the rats in model group. The body weight, tumor and spleen weight was weighed, then the ratio of tumor restriction and the index of spleen was calculated. MTT colorimetric method and ELISA were used to detected activity of T cell proliferation and expression of IL-2 in spleen. The expression of NF-κB and IκBα protein was detected by Western blot. Results The ratio of tumor restriction in the high, middle, low dose of KLT groups decreased gradually. The indexes of spleen of the high and middle dose of KLT groups were higher than those in the model group （P<0.05 or P<0.01）.Compared with the model group, the activity of T cell proliferation in the high, middle, low dose of KLT groups and the expression of IL-2 in the high and middle dose of KLT groups was increased significantly (P<005 or P<0.01). The expression of NF-κB protein in the nuclei of high, middle, low dose of KLT groups increased dose-dependently, and the expression of NF-κB and IκBα protein in the cytoplasm decreased dosedependently. Conclusion KLT could enhance immunological function by effecting T cell proliferation, expression of IL-2, NF-κB and IκBα, while restricting tumor growth in Lewis lung carcinoma models.

Key words: Kanglaite injection, Lewis lung carcinoma, immunological function, rat