上海交通大学学报（医学版）

›› 2009, Vol. 29 ›› Issue (12): 1482-.

• 论著（临床研究） • 上一篇    下一篇

胃癌组织中c-met、e2f-1和Ki-67的表达

伍宏彪1, 张 鹏1, 李小强2, 吴巨钢1, 俞继卫1, 姜波健1   

  1. 上海交通大学 医学院第三人民医院 1. 普外科, 2. 病理科, 上海 201900
  • 出版日期:2009-12-25 发布日期:2009-12-25
  • 通讯作者: 姜波健, 电子信箱: jiang_md@hotmail.com。
  • 作者简介:伍宏彪（1979—）, 男, 主治医师, 硕士；电子信箱: wuhongbiao@126.com。
  • 基金资助:

    上海交通大学医学院科研基金（2007XJ032, 2009XJ21037）和上海市科委基金（09411962300）

Expression of c-met, e2f-1 and Ki-67 in tissues of gastric cancer

WU Hong-biao1, ZHANG Peng1, LI Xiao-qiang2, WU Ju-gang1, YU Ji-wei1, JIANG Bo-jian1   

  1. 1. Department of General Surgery, 2. Department of Pathology, The Third People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 201900, China
  • Online:2009-12-25 Published:2009-12-25
  • Supported by:

    Foundation of Shanghai Jiaotong University School of Medicine, 2007XJ032, 2009XJ21037；Shanghai Science and Technology Committee Foundation, 09411962300

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摘要：

目的 检测肝细胞生长因子受体c-met、转录因子e2f-1和Ki-67抗原在胃癌组织中的表达，探讨三者的相关性及与临床病理特征和预后的关系。方法 免疫组织化学染色法检测86例胃癌患者手术标本中c-met、e2f-1和Ki-67的表达，单因素与多因素法分析其与临床病理特征的关系及三者的相关性，Log rank检验分析其与患者预后的关系。结果 c-met、e2f-1和Ki-67在胃癌组织中均广泛表达。c-met的高表达与Ki-67高表达、生存期较短、肿瘤位置较高、淋巴结转移率较高、累及淋巴站较远及TNM分期较晚有关(P<0.05)；e2f-1的低表达与肿瘤直径较大、TNM分期较晚、浸润较深、淋巴结转移率较高、累及淋巴站较远有关(P<0.05)。多因素分析显示，浸润深度、累及淋巴站、TNM分期和Ki-67表达是c-met表达的独立相关因素，而年龄和生存期是e2f-1表达的独立相关因素。Log rank检验显示，与术后生存相关的因素有c-met、e2f-1和Ki-67表达、累及淋巴站、肿瘤直径、肿瘤位置、淋巴结转移率、浸润深度和TNM分期。结论 c-met阳性表达的胃癌患者生存期显著缩短。e2f-1在早期胃癌中高表达，晚期胃癌的进展可能与e2f-1表达降低有关。

关键词: 胃癌, c-met, e2f-1, Ki-67, 预后

Abstract:

Objective To detect the expression of c-met (hepatocyte growth factor receptor), e2f-1 （transcription factor） and Ki-67 in tissues of gastric cancer, explore the relationship among them, and investigate their correlationship with clinicopathological characteristics and prognosis. Methods The tissue samples of gastric cancer from 86 patients with radical resection were subjected to immunohistochemical staining, and the expression of c-met, e2f-1 and Ki-67 was detected. The relationship between the clinicopathological characteristics and the expression of c-met, e2f-1 and Ki-67, and that among the expression of c-met, e2f-1 and Ki-67 were explored by univariate and multivariate data analysis. And the relationship between prognosis and expression of c-met, e2f-1 and Ki-67 was analysed by Log rank test. Results c-met, e2f-1 and Ki-67 were widely expressed in tissues of gastric cancer. The higher expression of c-met was associated with higher expression of Ki-67, shorter survival time, upper tumor location, higher lymph node metastasis rate, higher N stage and TNM stage (P<0.05), and the lower expression of e2f-1 was associated with larger tumor diameter, higher TNM stage, deeper invasion, higher lymph node metastasis rate and higher N stage (P<0.05). The multivariate analysis revealed that depth of invasion, N stage, TNM stage and expression of Ki-67 were independent factors for positive expression of c-met, and age and survival time were independent factors for positive expression of e2f-1. Log rank test demonstrated that the factors related to survival included expression of c-met, e2f-1 and Ki-67, N stage, tumor diameter, tumor location, lymph node metastasis rate, depth of invasion and TNM stage. Conclusion c-met is an indicator for malignant behavior and poorer prognosis of gastric cancer. There is a higher expression of e2f-1 in early gastric cancer, while advanced gastric cancer may be associated with a lower expression of e2f-1.

Key words: gastric cancer, c-met, e2f-1, Ki-67, prognosis