上海交通大学学报（医学版）

›› 2009, Vol. 29 ›› Issue (7): 817-.

• 论著（基础研究） • 上一篇    下一篇

脑深部电刺激对异动症大鼠纹状体DARPP-32及其磷酸化蛋白表达的影响

浦 政, 陆丽霞, 刘振国   

  1. 上海交通大学 医学院新华医院神经内科, 上海 200092
  • 出版日期:2009-07-25 发布日期:2009-09-16
  • 通讯作者: 刘振国, 电子信箱：zhenguoliu@yahoo.com.cn。
  • 作者简介:浦政（1969—）, 男, 副主任医师, 硕士；电子信箱: paddy_69@126.com。
  • 基金资助:

    教育部留学回国人员科研启动基金；上海市教委基金（06BZ048）；上海市浦江人才计划；上海市科委基金（07SP07005）

Effects of deep brain stimulation on expression of DARPP-32 and its phosphorylated proteins in corpus striatum of rats with dyskinesia

PU Zheng, LU Li-xia, LIU Zhen-guo   

  1. Department of Neurology, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China
  • Online:2009-07-25 Published:2009-09-16
  • Supported by:

    Scientific Research Foundation of the Ministry of Education for Returned Overseas Chinese Scholars; Shanghai Education Committee Foundation, 06BZ048; Shanghai Pujiang Talent Program; Shanghai Science and Technology Committee Foundation, 07SP07005

PDF (PC)

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摘要：

目的 探讨丘脑底核高频电刺激对帕金森病异动症大鼠纹状体区多巴胺和cAMP调节的磷蛋白（DARPP-32）及其磷酸化蛋白表达的影响。方法 制备帕金森病异动症大鼠模型，经丘脑底核高频电刺激（刺激组）后，测定大鼠损伤侧及损伤对侧纹状体区DARPP-32及其磷酸化蛋白的表达。另设假刺激组和假手术组为对照。结果 异动症大鼠纹状体区DARPP-32总蛋白的表达在三组之间比较，差异均无统计学意义（P＞0.05）。刺激组大鼠损伤侧纹状体区Phosphor-Thr34-DARPP-32蛋白表达明显低于假刺激组和假手术组（P<0.05）；刺激组大鼠损伤侧纹状体区Phosphor-Thr75-DARPP-32蛋白表达明显高于假刺激组和假手术组（P<0.05）。结论 DARPP-32及其磷酸化蛋白的表达变化在帕金森病异动症的发病中有重要作用。

关键词: 帕金森病, 异动症, 脑深部电刺激, 丘脑底核, 多巴胺和cAMP调节的磷蛋白

Abstract:

Objective To investigate the effects of subthalamic nucleus (STN)-deep brain stimulation (DBS) on expression of dopamine and adenosine 3’5’-nophosphate-egulated phosphor-rotein (DARPP-32) and its phosphorylated proteins in corpus striatum of rat models with levodopa-nduced dyskinesia. Methods The rat models of levodopa-nduced dyskinesia were set up and were given STN-DBS (stimulation group). The expression of DARPP-32 and its phosphorylated proteins in corpus striatum (damage side and normal side) were detected and compared with sham-stimulation group and sham-operation group. Results There was no significant difference in the expression of DARPP-32 total protein in corpus striatum of rats with dyskinesia among three groups (P>0.05). The expression of Phosphor-Thr34-DARPP-32 protein in the damage side of corpus striatum in stimulation group was significantly lower than that in sham-stimulation group and sham-operation group（P<0.05）, while the expression of Phosphor-Thr75-DARPP-32 protein in the damage side of corpus striatum in stimulation group was significantly higher than that in sham-stimulation group and sham-operation group（P<0.05）. Conclusion DARPP-32 and its phosphorylated proteins play an important role in the pathogenesis of levodopa-induced dyskinesia.

Key words: Parkinson´s disease, dyskinesia, deep brain stimulation, subthalamic nucleus, dopamine and adenosine 3’5’-monophosphate-regulated phosphor-protein