上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (1): 4-.

• 专题报道（营养学研究） • 上一篇    下一篇

益生菌干预对卵清蛋白过敏幼鼠模型的免疫调节作用

唐文静1, 黄 娟1, 钟 燕2, 周 侃2, 陈 兵2   

  1. 1. 上海交通大学 医学院新华医院临床营养中心, 上海 200092；2. 上海交通大学 医学院营养系, 上海 200025
  • 出版日期:2010-01-26 发布日期:2010-01-26
  • 通讯作者: 钟 燕, 电子信箱: zhongyan73@263.net。
  • 作者简介:唐文静（1986—）, 助教, 学士；电子信箱: tangwenjing1986@yahoo.com.cn。
  • 基金资助:

    国家自然科学基金（30600497）；上海交通大学医学院自然科学研究基金（2007XJ008）

Immunomodulatory effects of probiotics on rat models with ovalbumin induced food allergy

TANG Wen-jing1, HUANG Juan1, ZHONG Yan2, ZHOU Kan2, CHEN Bing2   

  1. 1. Clinical Nutrition Center, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China；2. Department of Nutrition, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2010-01-26 Published:2010-01-26
  • Supported by:

    National Natural Science Foundation of China, 30600497；Foundation of Shanghai Jiaotong University School of Medicine, 2007XJ008

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摘要：

目的 研究益生菌干预对卵清蛋白(OVA)诱导幼鼠食物过敏发生的影响，以及对模型动物免疫功能的调节作用。方法 3周龄断乳雌性Brown-Norway幼鼠随机分为空白对照组、食物过敏组和益生菌干预组，每组10只。采用ELISA法检测各组血清OVA-IgE和小肠内容物sIgA水平。体外分离培养脾淋巴细胞，采用流式细胞术对脾调节性T淋巴细胞亚群分类计数，采用ELISA法检测细胞上清液中IFN-γ、IL-4和IL-10的水平。结果 食物过敏组血清OVA-IgE质量浓度显著高于空白对照组（P<0.05），益生菌干预组OVA-IgE质量浓度显著低于食物过敏组（P<0.05）。益生菌干预组小肠内容物中sIgA水平显著高于食物过敏组和空白对照组（P<0.05）。食物过敏组脾CD4+CD25+T淋巴细胞的百分比显著低于空白对照组（P<0.05）；益生菌干预组与食物过敏组相比有升高趋势，但差异无统计学意义（P>0.05）。食物过敏组脾IL-4和IL-10的质量浓度显著高于空白对照组（P<0.05）；益生菌干预组IFN-γ、IL-4和IL-10的水平均显著低于食物过敏组（P<0.05）；食物过敏组IFN-γ/IL-4的比值显著低于空白对照组（P<0.05），而益生菌干预组显著高于食物过敏组（P<0.05）。结论 益生菌干预能够通过调节模型动物Th1/Th2细胞因子的平衡起到防治食物过敏的作用。

关键词: 食物过敏, 益生菌, 免疫调节, Th1/Th2细胞因子

Abstract:

Objective To investigate the effects of probiotics on the occurrence of ovalbumin (OVA) induced food allergy and the regulatory effects on immune function of rat models. Methods Thirty female Brown-Norway rats aged 3 weeks were randomly divided into blank control group, food allergy group and probiotics intervention group (n=10). The levels of serum OVA-IgE and intestinal sIgA were measured by ELISA method. Splenic lymphocytes were isolated and cultured in vitro, and the Treg lymphocyte subgroups in the spleen were analyzed by flow cytometry. The levels of IFN-γ, IL-4 and IL-10 in the supernatant of cultured splenic lymphocytes were measured by ELISA method. Results The serum OVA-IgE level in food allergy group was significantly higher than that in blank control group (P<0.05), while that in probiotics intervention group was significantly lower than that in food allergy group (P<0.05). Compared with food allergy group and blank control group, the level of intestinal sIgA in probiotics intervention group was significantly higher (P<0.05). The percentage of CD4+CD25+T lymphocytes in food allergy group was significantly lower than that in blank control group (P<0.05), and that in probiotics intervention group had the increase tendency compared with food allergy group, with no significant difference between probiotics intervention group and food allergy group (P>0.05). The levels of IL-4 and IL-10 in food allergy group were significantly higher than those in blank control group (P<0.05). Probiotics intervention group had significantly lower levels of IL-4, IL-10 and IFN-γ than food allergy group (P<0.05). The ratio of IFN-γ/IL-4 in food allergy group was significantly lower than that in blank control group (P<0.05), while that in probiotics intervention group was significantly higher than that in food allergy group (P<0.05).ConclusionProbiotics intervention could prevent the occurrence of food allergy in animal models by modulating the Th1/Th2 cytokine balance.

Key words: food allergy, probiotics, immunomodulation, Th1/Th2 cytokine