上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (1): 9-.

• 专题报道（营养学研究） • 上一篇    下一篇

诱导幼鼠食物过敏与哮喘发生的免疫学相关性研究

高 欣1, 陈 兵1, 唐文静2, 钟 燕2, 黄 娟1, 蔡 威1   

  1. 上海交通大学 医学院 1. 新华医院临床营养中心, 上海 200092；2. 营养系, 上海 200025
  • 出版日期:2010-01-26 发布日期:2010-01-26
  • 通讯作者: 钟 燕, 电子信箱: zhongyan73@263.net。
  • 作者简介:高 欣（1986—）, 女, 硕士生；电子信箱: gx_chris@yahoo.cn。
  • 基金资助:

    国家自然科学基金（30600497）和上海交通大学医学院创新基金（08012）

Immunological relationship between food allergy induction and occurrence of asthma in mice

GAO Xin1, CHEN Bing1, TANG Wen-jing2, ZHONG Yan2, HUANG Juan1, CAI Wei1   

  1. 1. Clinical Nutrition Center, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China；2. Department of Nutrition, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2010-01-26 Published:2010-01-26
  • Supported by:

    National Natural Science Foundation of China, 30600497；Shanghai Jiaotong University School of Medicine Foundation, 08012

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摘要：

目的 研究早期诱导小鼠食物过敏与后期哮喘发生的免疫相关性及其肺部组织病理学改变。方法 6周龄断乳雌性BALB/c幼鼠（n=37）随机分为3组，分别为空白对照组（n=12），食物过敏组（n=13）和哮喘组（n=12）。造模结束后各组均采用卵清蛋白（OVA）雾化激发，检测各组血清IgE水平、支气管肺泡灌洗液（BALF）中细胞因子IL-4、INF-γ水平，计算BALF中炎性细胞与嗜酸性粒细胞（EOS）数量。取小鼠肺部组织做病理切片，观察支气管管壁厚度和EOS浸润。结果 食物过敏组与哮喘组血清IgE水平、BALF中IL-4水平、IL-4/INFγ比值和EOS计数均显著高于空白对照组（P<0.05），哮喘组BALF中IL-4水平和EOS计数显著高于食物过敏组（P<0.05），而两组间IgE水平差异无统计学意义（P>0.05）；食物过敏组与哮喘组BALF中IFN-γ水平显著低于空白对照组（P<0.05）。肺组织病理观察显示，食物过敏组与哮喘组肺部组织嗜酸性细胞浸润均明显增加（P<0.05），支气管管壁增厚，哮喘组EOS浸润数显著高于食物过敏组（P<0.05）。结论 食物过敏组和哮喘组模型均存在IgE介导的全身免疫应答增高，食物过敏所致的肺部Th1/Th2免疫失衡和炎性细胞浸润可能是后期哮喘发生的免疫学机制。

关键词: 食物过敏, 哮喘, 嗜酸性粒细胞, Th1/Th2细胞因子, 炎性细胞

Abstract:

Objective To investigate immunological relationship between early induction of food allergy and occurrence of later asthma in mice, and explore the pathological changes in lung tissues. Methods Thirty-seven female BALB/c mice aged 6 weeks were randomly divided into blank control group (n=12), food allergy group (n=13) and asthma group (n=12). After being challenged by ovalbumin (OVA), the levels of serum IgE, IL-4 and INF-γ in bronchoalveolar lavage fluid (BALF) were detected. The numbers of inflammatory cells and eosinophils (EOS) in BALF were counted. Lung tissues were obtained for pathological sections, and thickness of bronchial wall and EOS infiltration were observed. Results The level of serum IgE and level of IL-4, ratio of IL-4/IFN-γ and number of EOS in BALF in food allergy group and asthma group were significantly higher than those in control group (P<0.05). The level of IL-4 and number of EOS in BALF in asthma group were significantly higher than those in food allergy group (P<0.05), while there was no significant difference in serum IgE level between these two groups （P>0.05）, and levels of IFN-γ in BALF in both groups were significantly lower than that in blank control group (P<0.05). There were more EOS infiltration in lung tissues and thicker bronchial wall in food allergy group and asthma group than that in blank control group (P<0.05), and the number of EOS in asthma group was significantly higher than that in food allergy group (P<0.05). Conclusion IgE-mediated immune response is involved in both food allergy and asthma mouse models. Lung immune imbalance of Th1/Th2 and inflammatory cell infiltration caused by food allergy may participate in the occurrence of later asthma.

Key words: food allergy, asthma, eosinophil, Th1/Th2 cytokine, inflammatory cell