上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (3): 259-.

• 专题报道（甲状腺癌核素治疗） • 上一篇    下一篇

分化型甲状腺癌细胞株FTC-133失分化现象与131I辐射的关系

冯 方, 傅宏亮, 叶智轶, 杜学亮, 王 辉   

  1. 上海交通大学 医学院新华医院核医学科, 上海 200092
  • 出版日期:2010-03-25 发布日期:2010-03-24
  • 通讯作者: 王 辉, 电子信箱: wanghuishanghai@hotmail.com。
  • 作者简介:冯 方（1984—), 男, 住院医师, 硕士；电子信箱: fengfang0815@163.com。
  • 基金资助:

    上海市重点学科建设项目（S30203）

Dedifferentiation of differentiated thyroid carcinoma cell line FTC-133 and its relationship with 131I radiation

FENG Fang, FU Hong-liang, YE Zhi-yi, DU Xue-liang, WANG Hui   

  1. Department of Nuclear Medicine, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China
  • Online:2010-03-25 Published:2010-03-24
  • Supported by:

    Shanghai Leading Academic Discipline Project, S30203

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摘要：

目的 研究体外培养的分化型甲状腺癌细胞株FTC-133失分化现象与放射性碘（131I）辐射的关系。方法 体外培养的甲状腺癌细胞株FTC-133分为实验组（以经筛选不造成细胞增殖抑制剂量的Na131I辐射细胞48 h，长期传代培养）、对照组（不加入Na131I，长期传代培养）和空白组（细胞冻存，实验时复苏）。测定各组细胞摄碘率；分别采用RT-PCR、Western blotting和放射免疫分析法（RIA）检测各组细胞甲状腺球蛋白 (Tg)、甲状腺过氧化物酶(TPO)、钠碘转运体(NIS)、促甲状腺激素受体（TSHR）mRNA和蛋白表达。结果 细胞摄碘率为空白组>对照组>实验组，组间比较差异均有统计学意义（P<0.01）。与空白组比较，实验组和对照组细胞Tg、TPO、NIS、TSHR蛋白表达均明显下降，且以实验组下降尤为显著，组间比较差异均有统计学意义（P<0.05）；三组Tg、TPO、NIS和TSHR mRNA与蛋白表达变化一致。结论 分化型甲状腺癌细胞FTC-133长期体外培养自动经历失分化过程，131I辐射促进这一过程的发展，可能与甲状腺特异蛋白表达下降有关。

关键词: 分化型甲状腺癌, 失分化, 放射性碘, 辐射

Abstract:

Objective To investigate the relationship between dedifferentiation of differentiated thyroid carcinoma (DTC) cell line FTC-133 cultured in vitro and radioactive iodine (131I) radiation. Methods DTC FTC-133 cells cultured in vitro were divided into experiment group (long-term cultivation after Na131I radiation for 48 h), control group (long-term cultivation without Na131I radiation) and blank group (cryopreservation and revitalization for experiment). Iodide uptake of each group was determined, and RT-PCR, Western blotting and radioimmunoassay (RIA) were employed to detect the expression of thyroglobulin (Tg), thyroid peroxidase (TPO), sodium-iodide symporter (NIS) and thyroid-stimulating hormone receptor (TSHR) mRNA and protein. Results The iodide uptake rate of blank group was the highest and that of experiment group was the lowest, with significant differences among groups (P<0.01). Compared with blank group, the expression of Tg, TPO, NIS and TSHR protein in experiment group and control group decreased, especially in experiment group, with significant differences among groups (P<0.05). The changes of expression of Tg, TPO, NIS and TSHR mRNA were in accordance with those of expression of Tg, TPO, NIS and TSHR protein. Conclusion DTC FTC133 cells may experience dedifferentiation during long-term in vitro culture, and 131I radiation may promote this process, which may be associated with decreased expression of thyroid specific protein.

Key words: differentiated thyroid carcinoma, dedifferentiation, radioactive iodine, radiation