上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (3): 296-.

• 论著（基础研究） • 上一篇    下一篇

不同性发育期雌性大鼠下丘脑组织NELL2和GnRH mRNA表达研究

段文凤, 李 嫔   

  1. 上海交通大学 上海市儿童医院内分泌科, 上海 200040
  • 出版日期:2010-03-25 发布日期:2010-03-24
  • 通讯作者: 李 嫔, 电子信箱: lipin21@126.com。
  • 作者简介:段文凤（1984—）, 女, 硕士生；电子信箱: duanwenfeng1984@126.com。
  • 基金资助:

    上海市卫生局基金资助（2008-05）

Analysis of expression of NELL2 and GnRH mRNA in hypothalamus of female SD rats at different sex development stages

DUAN Wen-feng, LI Pin   

  1. Department of Endocrinology, Children's Hospital, Shanghai Jiaotong University, Shanghai 200040, China
  • Online:2010-03-25 Published:2010-03-24
  • Supported by:

    Shanghai Municipal Health Burean Foundation, 2008-05

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摘要：

目的 研究不同性发育期雌鼠下丘脑组织中NELL2和GnRH mRNA表达的变化。方法 SD雌鼠按日龄分为幼年组（20日龄）、青春期前组（30日龄）、青春早期组（35日龄）和性发育成熟期组（45日龄），每组10只。Real-time PCR检测各组雌鼠下丘脑组织NELL2、GnRH mRNA 表达，并进行组间比较和分析。结果 在幼年组、青春期前组、青春早期组和性发育成熟期组雌鼠的下丘脑组织中，NELL2 mRNA相对表达分别为－1.01±0.09、－0.78±0.16、－1.09±0.10和－1.14±0.12；GnRH mRNA相对表达分别为－2.92±0.15、－2.44±0.10、－1.66±0.13和－2.13±0.19。统计学分析表明，NELL2 mRNA 表达为青春期前组>幼年组>青春早期组>性发育成熟期组，各组间比较差异均有统计学意义(P<0.05)；GnRH mRNA表达为青春早期组>性发育成熟期组>青春期前组>幼年组，各组间比较差异均有统计学意义(P<0.05)。结论 不同性发育期雌鼠下丘脑组织中NELL2和GnRH mRNA表达水平明显不同；与GnRH mRNA比较，NELL2 mRNA表达高峰出现较早。提示在青春期前，NELL2可能通过上调GnRH神经元GnRH mRNA表达而参与性发育启动。

关键词: 性发育, NELL2, GnRH, 基因表达, 大鼠, 雌性

Abstract:

Objective To investigate the changes of expression of NELL2 and GnRH mRNA in hypothalamus of female SD rats at different sex development stages. Methods SD rats were assigned to juvenile group (20 days old), prepubertal group (30 days old), early pubertal group (35 days old) and adult stage group (45 days old), with ten rats in each group. Real-time PCR was employed to detect the expression of NELL2 and GnRH mRNA in hypothalamus of rats in each group, and comparison was made among groups. Results The relative expression of NELL2 mRNA in hypothalamus of rats in juvenile group, prepubertal group, early pubertal group and adult stage group was －1.01±0.09, －0.78±0.16, －1.09±0.10 and －1.14±0.12, respectively, and that of GnRH mRNA was －2.92±0.15, －2.44±0.10, －1.66±0.13 and －2.13±0.19, respectively. Statistic analysis revealed that the expression of NELL2 mRNA was the highest in prepubertal group, followed by juvenile group, early pubertal group and adult stage group, with significant differences among groups (P<0.05). The expression of GnRH mRNA was the highest in early pubertal group, followed by adult stage group, prepubertal group and juvenile group, with significant differences among groups (P<0.05). Conclusion There were significant differences in expression of NELL2 and GnRH mRNA in hypothalamus of female SD rats at different sex development stages. The higher expression of NELL2 mRNA comes earlier than that of GnRH mRNA. In prepubertal stage, NELL2 may participate in the puberty onset via up-regulating the expression of GnRH mRNA.

Key words: sex development, NELL2, GnRH, gene expression, rat, female