上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (4): 370-.

• 论著（基础研究） • 上一篇    下一篇

尿道修复支架材料生物力学性质比较

冯 超1, 徐月敏1, 朱卫东1, 崔 磊2   

  1. 1. 上海交通大学 第六人民医院泌尿外科, 上海 200233；2. 上海市组织工程研究与开发中心, 上海 200235
  • 出版日期:2010-04-25 发布日期:2010-04-26
  • 通讯作者: 徐月敏, 电子信箱: xuyuemin@263.net。
  • 作者简介:冯 超（1981—）, 男, 博士生；电子信箱: fengchao9901790@yahoo.com.cn。
  • 基金资助:

    国家自然科学基金(30772199)

In vitro assessment of mechanical properties of scaffolds for urethral reconstruction

FENG Chao1, XU Yue-min1, ZHU Wei-dong1, CUI Lei2   

  1. 1. Department of Urology, The Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China；2. Shanghai Tissue Engineering Research &|Development Center, Shanghai 200235, China
  • Online:2010-04-25 Published:2010-04-26
  • Supported by:

    National Natural Science Foundation of China, 30772199

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摘要：

目的 检测并比较多种组织工程尿道修复支架材料的生物力学性质。方法 制备小肠黏膜下组织（SIS）、膀胱黏膜下脱细胞基质（BAMG）、聚羟乙酸(PGA)和尿道海绵体脱细胞基质（ACSM）支架材料，对其进行大体观察、HE染色及Masson染色观察。于单向拉伸实验机上检测单层SIS组、BAMG冻干保存组、BAMG液体保存组、ACSM组、PGA组、兔正常尿道标本组和4SIS组（SIS经4层叠加）的Young氏模量、最大载荷、抗拉强度和最大伸长率等生物力学参数，并进行组间比较。结果 ACSM的厚度与其他支架材料有明显差异，HE和Masson染色显示其由更为丰富的胶原构成。生物力学检测结果显示，Young氏模量：ACSM组>PGA 组>4SIS组>BAMG液体保存组>BAMG冻干保存组>兔正常尿道组>SIS组;最大载荷：ACSM组>4SIS组>PGA组>BAMG液体保存组>BAMG冻干保存组>兔正常尿道组>SIS组;抗拉强度：ACSM组>4SIS组>PGA组>BAMG液体保存组>BAMG冻干保存组>兔正常尿道组>SIS组；最大伸长率：兔正常尿道组>SIS组>BAMG冻干保存组>4SIS组> BAMG液体保存组>ACSM组>PGA组。ACSM组在Young氏模量、最大载荷和抗拉强度上显著优于其他组（P<0.01）。结论 异种ACSM支架具有良好的生物力学性质，较SIS、BAMG和PGA等支架更适用于尿道重建。

关键词: 支架, 力学, 组织工程

Abstract:

Objective To determine and compare the mechanical properties of several biomaterials used for urethral reconstruction. Methods Materials for scaffolds such as bladder acellular matrix graft (BAMG), small intestinal submucosa (SIS), acellular corpus spongiosum matrix (ACSM) and polyglycolic acid (PGA) were prepared, and gross observation, HE and Masson staining were performed. The Young's modulus, maximal load, tensile strength, elongation at break were tested and compared among SIS group, BAMG freeze-drying preservation group, BAMG liquid preservation group, ACSM group, PGA group, normal rabbit urethra group and 4SIS group (4-layer SIS). Results The thickness of ACSM was different from the other materials, and richer and denser collagen was observed in ACSM by HE and Masson staining. The results of mechanical property tests were: ACSM group>PGA group>4SIS group>BAMG liquid preservation group>BAMG freeze-drying preservation group>normal rabbit urethra group>SIS group for Young's modulus; ACSM group>4SIS group>PGA group>BAMG liquid preservation group>BAMG freeze-drying preservation group>normal rabbit urethra group>SIS group for maximal load; ACSM group>4SIS group>PGA group>BAMG liquid preservation group>BAMG freeze-drying preservation group>normal rabbit urethra group >SIS group for tensile strength; normal rabbit urethra group>SIS group>BAMG freeze-drying preservation group>4SIS group>BAMG liquid preservation group>ACSM group>PGA group for elongation at break. The properties of Young's modulus, maximal load and tensile strength in ACSM group were significantly better than those of the other groups (P<0.01). Conclusion ACSM enjoys excellent mechanical properties, and may perform better than SIS, BAMG and PGA in urethral reconstruction.

Key words: scaffold, mechanical property, tissue engineering