›› 2010, Vol. 30 ›› Issue (4): 419-.

• 论著(基础研究) • 上一篇    下一篇

寻常型天疱疮皮损组织中Th17细胞相关细胞因子的表达

苏文婷1, 王宏林2, 费 烨1, 汪蓓青1, 徐 慧1, 陈向东1   

  1. 上海交通大学 医学院 1. 第九人民医院皮肤科, 上海 200011, 2. 上海市免疫学研究所, 上海 200025
  • 出版日期:2010-04-25 发布日期:2010-04-26
  • 通讯作者: 陈向东, 电子信箱: xdchen@medmail.com.cn。
  • 作者简介:苏文婷(1986—), 女, 硕士生;电子信箱: suwenting1986@yahoo.com.cn。

Expression of Th17 cell associated cytokines in pemphigus vulgaris lesions

SU Wen-ting1, WANG Hong-lin2, |FEI Ye1, WANG Bei-qing1, XU Hui1, CHEN Xiang-dong1   

  1. 1. Department of Dermatology, The Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200011, China;2. Shanghai Institute of Immunology, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2010-04-25 Published:2010-04-26

摘要:

目的 研究Th17细胞相关细胞因子IL-23和IL-17在寻常型天疱疮(PV)发病机制中的作用。方法 采用免疫荧光染色法分析PV患者皮损组织和健康人皮肤组织中Th17细胞相关因子IL-17和IL-23的表达及CD163+巨噬细胞的分布。结果 IL-23+细胞的染色与CD163+巨噬细胞的染色重叠。PV患者真皮中IL-23+CD163+细胞和IL-17+细胞在每个视野(×200)中的平均数分别为80.17±5.42和48.17±8.03,而正常人真皮中分别为29.50±4.45和9.50±3.35,两组差异均有统计学意义(P<0.01)。真皮层中IL-23+细胞数和IL-17+细胞数存在相关性(r=0.945,P<0.001)。结论 CD163+巨噬细胞是IL-23重要分泌源;IL-23和IL-17在PV患者皮损组织中高表达,IL-23/IL-17细胞因子轴在PV发病机制中可能起着非常重要的作用。

关键词: 寻常型天疱疮, 巨噬细胞, Th17细胞, IL-23, IL-17

Abstract:

Objective To investigate the role of Th17 cell associated cytokines of IL-23 and IL-17 in the pathogenesis of pemphigus vulgaris. Methods Immunofluorescence staining was performed to analyse the distribution of CD163+ macrophages and expression of IL-23 and IL-17 in pemphigus vulgaris lesions and healthy human skin tissues. Results The staining of IL-23+cells is overlapped with that of CD163+ macrophages. The mean numbers of IL23+CD163+ cells and IL-17+ cells were 80.17±5.42 and 48.17±8.03 in each field of view (×200) in dermis of pemphigus vulgaris, while those in healthy human dermis were 29.50±4.45 and 9.50±3.35, with significant differences between dermis of pemphigus vulgaris and healthy human dermis (P<0.01). The number of IL23+ cells was related to that of IL-17+ cells in dermis (r=0.945, P<0.001). Conclusion IL-23 is mainly secreted by CD163+ macrophages. IL-23 and IL-17 are overexpressed in pemphigus vulgaris lesions. IL-23/IL-17 cytokine axis may play an important role in the pathogenesis of pemphigus vulgaris.

Key words: pemphigus vulgaris, macrophages, Th17 cell, IL-23, IL-17