›› 2010, Vol. 30 ›› Issue (6): 669-.

• 论著(基础研究) • 上一篇    下一篇

重组人生长激素对肝癌细胞MHCC-97H的作用及机制

朱晨芳, 钟 强, 顾 岩   

  1. 上海交通大学 医学院第九人民医院普外科, 上海 200011
  • 出版日期:2010-06-25 发布日期:2010-06-28
  • 通讯作者: 顾 岩, 电子信箱: yangu@sjtu.edu.cn。
  • 作者简介:朱晨芳(1977—), 女, 主治医师, 硕士;电子信箱: sammizz1977@sina.com。
  • 基金资助:

    上海市卫生局基金(054052);上海市科委登山计划重点项目基金(06DZ19506)

Effects of human recombinant growth hormone on hepatocellular carcinoma cell line MHCC-97H and underlying mechanism

ZHU Chen-fang, ZHONG Qiang, GU Yan   

  1. Department of General Surgery, The Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200011, China
  • Online:2010-06-25 Published:2010-06-28
  • Supported by:

    Shanghai Municipal Health Bureau Foundation, 054052;Shanghai Science and Technology Committee Foundation, 06DZ19506

摘要:

目的 探讨重组人生长激素(rhGH)对肝癌细胞MHCC-97H的作用及机制。方法 以无血清培养液诱导肝癌细胞MHCC-97H凋亡,再以浓度为0 μg/L(GH0组)、5 μg/L(GH5组)、25 μg/L(GH25组)、50 μg/L(GH50组)、100 μg/L(GH100组)和500 μg/L(GH500组) 的rhGH完全培养液进行培养,MTT法观察MHCC-97H细胞生长情况,流式细胞技术检测细胞凋亡率、细胞周期和细胞增殖指数,RT-PCR和Western blotting法分别检测凋亡相关基因Bax和Bcl-2 mRNA和蛋白表达情况。 结果 与GH0组比较,GH25组、GH50组、GH100组和GH500组MHCC-97H细胞增殖明显,S期细胞百分比和细胞增殖指数显著增加 (P<0.05);GH25组、GH50组和GH100组细胞凋亡率显著降低(P<0.05),Bax mRNA和蛋白表达显著降低(P<0.05),Bcl-2 mRNA和蛋白表达显著增加(P<0.05)。结论 rhGH体外显著促进肝癌细胞MHCC-97H增殖,并通过调节凋亡相关基因Bax和Bcl-2的表达来抑制无血清培养液诱导的细胞凋亡。

关键词: 重组人生长激素, 肝癌细胞, 凋亡, Bax, Bcl-2

Abstract:

Objective To investigate the effects of recombinant human growth hormone (rhGH) on hepatocellular carcinoma cell line MHCC-97H, and explore the underlying mechanism. Methods Apoptosis of hepatocellular carcinoma cell line MHCC-97H was induced by serum free culture fluid, and MHCC-97H cells were then cultured with 0 μg/L rhGH (GH0 group), 5 μg/L rhGH (GH5 group), 25 μg/L rhGH (GH25 group), 50 μg/L rhGH (GH50 group), 100 μg/L rhGH (GH100 group) and 500 μg/L rhGH (GH500 group), respectively. The growth of MHCC-97H cells was observed by MTT method, cell apoptosis rate, cell cycle and cell proliferation index were determined by flow cytometry, and RT-PCR and Western blotting were employed to detect the expression of Bax and Bcl-2 mRNA and protein, respectively. Results Compared with GH0 group, the proliferation of MHCC-97H cells, the percentages of cells in S phase and proliferation indexes significantly increased in GH25 group, GH50 group, GH100 group and GH500 group (P<0.05). Cell apoptosis rates in GH25 group, GH50 group and GH100 group significantly decreased (P<0.05), the expression of Bax mRNA and protein significantly decreased and that of Bcl-2 mRNA and protein significantly increased (P<0.05). Conclusion rhGH can stimulate the in vitro proliferation of hepatocellular carcinoma cell line MHCC-97H, the mechanism of which is closely related to the expression of Bax and Bcl-2 genes.

Key words: recombinant human growth hormone, hepatocellular carcinoma, apoptosis, Bax, Bcl-2