上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (6): 722-.

• 论著（临床研究） • 上一篇    下一篇

肾脏上皮样血管平滑肌脂肪瘤的免疫组织化学检测

王兆亮1,2, 朱建善2, 陈以明2, 王 莉1   

  1. 上海交通大学 1.基础医学院病理学教研室, 上海 200025；2.医学院仁济医院病理科, 上海 200127
  • 出版日期:2010-06-25 发布日期:2010-06-28
  • 通讯作者: 王 莉, 电子信箱: jywangl@shsmu.edu.cn。
  • 作者简介:王兆亮（1970—）, 女, 副主任医师, 硕士生；电子信箱: pomelo888@sina.com。

Immunohistochemical detection of epithelioid angiomyolipoma of kidney

WANG Zhao-liang1,2, ZHU Jian-shan2, CHEN Yi-ming2, WANG Li1   

  1. 1.Department of Pathology, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China；2.Department of Pathology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, China
  • Online:2010-06-25 Published:2010-06-28

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摘要：

目的 探讨一组免疫组织化学标志物在肾脏上皮样血管平滑肌脂肪瘤（EAML）中的表达及其对于诊断、鉴别诊断和预后的意义。方法 采用免疫组织化学EnVision二步法检测13例肾脏EAML中标志物vimentin、HMB45、Melan-A、AE1/AE3、EMA、SMA、ER、PR、CD68、CD10、CD34、CD117、S-100蛋白、P53和Ki-67的表达情况。结果 13例肿瘤细胞vimentin、SMA、HMB45、Melan-A、CD68均阳性；AE1/AE3、EMA、CD10、CD34、CD117、ER和PR均阴性；Ki-67和P53各有3例阳性；S-100蛋白有2例阳性。结论 免疫组织化学标志物SMA、HMB45、Melan-A、AE1/AE3、EMA可作为EAML诊断及鉴别诊断的首选抗体，Ki-67和P53对于患者的预后可能有指示意义。

关键词: 肾脏肿瘤, 上皮样血管平滑肌脂肪瘤, 免疫组织化学, 诊断

Abstract:

Objective To investigate the expression of a panel of immunohistochemical markers in epithelioid angiomyolipoma (EAML) of the kidney, and explore its roles in the diagnosis, differential diagnosis and prognosis. Methods The expression of  vimentin, HMB45, Melan-A, AE1/AE3, EMA, SMA, ER, PR, CD68, CD10, CD34, CD117, S-100 protein, P53 and Ki-67 was detected in 13 cases of renal EAMLs by immunochemical EnVision method. Results All the 13 cases of tumor cells were positive for vimentin, SMA, HMB45, Melan-A and CD68, while negative for AE1/AE3, EMA, CD10, CD34, CD117, ER and PR. Three cases of tumor cells were positive for Ki-67 and P53, and 2 were positive for S-100 protein. Conclusion Immunohistochemical markers of SMA, HMB45, Melan-A, AE1/AE3 and EMA can be used as primary antibodies for diagnosis and differential diagnosis of EAML of the kidney. Ki-67 and P53 might be valuable indexes in prognosis prediction.

Key words: kidney neoplasm, epithelioid angiomyolipoma, immunohistochemistry, diagnosis