上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (7): 942-.doi: 10.3969/j.issn.1674-8115.2011.07.016

• 论著（临床研究） • 上一篇    下一篇

p73基因DNA甲基化与儿童急性白血病的关系

张 妍1, 高 宇1, 王筱金2, 朱 莎3, 施 蓉1, 金 萍1, 杨 友3, 田 英1,3   

  1. 1.上海交通大学 公共卫生学院, 上海 200025； 2.上海交通大学 基础医学院生物统计学教研室, 上海 200025；3.上海交通大学 医学院附属新华医院 上海市环境与儿童健康重点实验室, 上海 200092
  • 出版日期:2011-07-28 发布日期:2011-07-27
  • 通讯作者: 田 英, 电子信箱: tianmiejp@yahoo.com.cn。
  • 作者简介:张 妍（1985—）, 女, 硕士生；电子信箱: yanyezi262@yahoo.com.cn。
  • 基金资助:

    国家自然科学基金（30801345, 30901171）；上海市自然科学基金（09ZR1416500）；上海交通大学医学院科技基金（09XJ21054）

Relationship between DNA methylation of p73 gene and acute leukemia of children

ZHANG Yan1, GAO Yu1, WANG Xiao-jin2, ZHU Sha3, SHI Rong1, JIN Ping1, YANG You3, TIAN Ying1,3   

  1. 1.School of Public Health, Shanghai Jiaotong University, Shanghai 200025, China；2.Department of Biostatistics, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China；3.Shanghai Key Laboratory of Children's Environmental Health, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China
  • Online:2011-07-28 Published:2011-07-27
  • Supported by:

    National Natural Science Foundation of China, 30801345, 30901171；Shanghai Natural Science Foundation, 09ZR1416500；Shanghai Jiaotong University School of Medicine Foundation, 09XJ21054

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摘要：

目的 探讨p73基因DNA甲基化在儿童急性白血病发病中的作用。方法 选取142例（年龄<15岁）初发急性白血病儿童作为病例组，其中急性淋巴细胞白血病（ALL）112例，急性髓性白血病（AML）30例；以接受健康体检或就诊的性别、年龄匹配的123儿童（排除血液系统疾病和肿瘤性疾病）作为对照组。采用甲基化特异性聚合酶链反应（MSP）分析病例组与对照组儿童p73基因的甲基化状态。随机选取p73基因DNA甲基化片段与非甲基化片段样本各1例进行PCR产物序列分析。结果 病例组p73基因DNA甲基化发生率（7.7%）显著高于对照组（0.8%），差异有统计学意义（P<0.05）；病例组和对照组p73基因缺失率分别为6.3% 和4.1%，组间比较差异无统计学意义（P>0.05）。病例组中ALL患儿的p73基因DNA甲基化发生率为8.9%，显著高于对照组（P<0.01）。随机选取的2个样本的测序结果与MSP结果一致。结论 p73基因DNA甲基化可能在儿童急性白血病尤其是ALL的发病中发挥重要作用。

关键词: p73, 急性白血病, 儿童, DNA甲基化

Abstract:

Objective To investigate the relationship between DNA methylation of p73 gene and acute leukemia of children. Methods One hundred and forty-two children with newly diagnosed acute leukemia aged less than 15 years were selected as case group, among whom 112 were acute lymphoblastic leukemia (ALL) and 30 were acute myeloid leukemia (AML). Another 123 sex and age-matched children undergoing physical examinations, outpatient visit or treatment without hematological diseases and tumors were served as control group. The methylation status of p73 gene of children in case group and control group was analysed by methylation-specific polymerase chain reaction (MSP). One sample of DNA methylated fragment and one sample of DNA non-methylated fragment of p73 gene were randomly selected for sequencing analysis of PCR product. Results The prevalence of DNA methylation of p73 gene in case group was significantly higher than that in control group （7.7% vs 0.8%, P<0.05）, while there was no significant difference in the deletion rate of p73 gene between case group and control group (6.3% vs 4.1%, P>0.05). The prevalence of DNA methylation of p73 gene in children with ALL in case group (8.9%) was significantly higher than that in control group (P<0.01). The sequencing results of two randomly selected samples were consistent with results of MSP. Conclusion DNA methylation of p73 gene may play an important role in the development of acute leukemia of children, especially in the development of ALL.

Key words: p73, acute leukemia, child, DNA methylationp