上海交通大学学报(医学版)

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孕期补锌对母胎汞毒性的拮抗作用及其抗氧化损伤机制研究

朱 泓1,2,杨祖菁2,惠 宁1,刘军霞3   

  1. 1.第二军医大学附属长海医院妇产科, 上海 200433; 2.上海交通大学 医学院附属新华医院妇产科, 上海 200092; 3.上海交通大学 医学院附属新华医院教育部和上海市环境与儿童健康重点实验室, 上海 200092
  • 出版日期:2014-02-28 发布日期:2014-03-25
  • 通讯作者: 惠 宁, 电子信箱: huin1956@163.com。
  • 作者简介:朱 泓(1979—), 女, 主治医师, 博士生; 电子信箱: wendybonjour@hotmail.com。

Study on antagonistic effects of zinc supplementation during pregnancy on mother-infant mercury toxicity and its anti-oxidative damage mechanism

ZHU Hong1,2, YANG Zu-jing2, HUI Ning1, LIU Jun-xia3   

  1. 1.Department of Obstetrics and Gynecology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; 2.Department of Obstetrics and
    Gynecology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 3.MOE-Shanghai Key Laboratory of Children′s
    Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
  • Online:2014-02-28 Published:2014-03-25

摘要:

目的 探讨孕期补锌对孕妇及胎儿的汞毒性的拮抗作用及其抗氧化损伤机制。方法 根据孕中、晚期孕妇的血汞值选取病例组、治疗组和对照组,每组30例。比较3组孕妇的血汞、血锌水平以及新生儿生长发育指标;应用脂质过氧化试剂盒检测3组胎盘的谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和丙二醛(MDA)表达水平。结果 治疗组孕晚期母血中汞质量浓度显著低于病例组,锌质量浓度高于病例组,差异均有统计学意义(P<0.05);治疗组孕晚期母血中汞质量浓度低于孕中期,锌质量浓度高于孕中期,差异也均有统计学意义(P<0.05)。治疗组新生儿的头围、身长、出生体质量优于病例组,差异均有统计学意义 (P<0.05)。治疗组胎盘的GSH-PX、SOD和CAT活性高于病例组,但低于正常组;治疗组胎盘的MDA含量低于病例组,但高于正常组,差异均有统计学意义 (P<0.05)。结论 孕期补锌可拮抗汞对孕妇及胎儿的毒性作用,锌的抗氧化损伤作用是其重要机制之一。

关键词: 锌, 汞, 新生儿生长发育, 谷胱甘肽过氧化物酶, 超氧化物歧化酶, 过氧化氢酶, 丙二醛

Abstract:

Objective To investigate the antagonism of zinc to mercury caused toxicity in mothers and fetuses and its anti-oxidation mechanism. Methods According to the levels of maternal blood mercury at middle and late pregnancy, 90 pregnant women were divided equally into the case group, the treatment group, and the control group. The levels of maternal blood mercury and zinc, and neonatal growth and development indexes of three groups were compared. The levels of glutathione peroxidase (GSH-PX), superoxidase dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) in placenta were detected by lipid peroxidation kit. Results The level of maternal blood mercury at late pregnancy of the treatment group was lower than that of the case group, while the level of zinc was higher than that of the case group and the difference was statistically significant (P<0.05). The level of maternal blood mercury of the treatment group at late pregnancy was lower than that
at middle pregnancy, while the level of zinc was higher than that at middle pregnancy and the difference was also statistically significant (P<0.05). The head circumference, crown-heel length, and birth weight of neonates of the case group were significantly lower than those of the treatment group in which the data were better (P<0.05). The levels of GSH-PX, SOD, and CAT in placenta of the treatment group were higher than those of the case group, but lower than those of the control group. The level of MDA in placenta of the treatment group was lower than that of the case group, but higher than that of the control group, and the differences were statistically significant (P<0.05). Conclusion Zinc supplement during pregnancy can antagonize mercury caused toxicity in mothers and fetuses. Antioxidant effect of zinc may be one of the important mechanisms.

Key words: zinc, mercury, neonatal growth and development, glutathione peroxidase, superoxidase dismutase, catalase, malondialdehyde