上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 技术与方法 • 上一篇    下一篇

不同脱钙液对钙化性主动脉瓣膜脱钙效果的比较

李灿波，李海清，叶晓峰，赵强   

  1. 上海交通大学 医学院附属瑞金医院心脏外科， 上海 200025
  • 出版日期:2015-08-28 发布日期:2015-09-30
  • 通讯作者: 赵强, 电子信箱: zq11607@rjh.com.cn。
  • 作者简介:李灿波（1988—）， 男， 硕士生; 电子信箱: untitledlcb@163.com。
  • 基金资助:

    国家自然科学基金（81200093，81000096）

Comparison of effects of different decalcification agents on calcific aortic valves

LI Can-bo, LI Hai-qing, YE Xiao-feng, ZHAO Qiang   

  1. Department of Cardiac Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025， China
  • Online:2015-08-28 Published:2015-09-30
  • Supported by:

    National Natural Science Foundation of China， 81200093， 81000096

PDF (PC)

879

摘要：

目的  比较不同脱钙液对钙化性主动脉瓣膜标本的脱钙效果。方法  选择6例主动脉瓣钙化患者的病变瓣膜标本，每个瓣膜等分成3份后随机分配到5%硝酸（硝酸组）、10%甲酸（甲酸组）、10%EDTA （EDTA组）溶液中进行脱钙，观察和比较各组病理切片的苏木精-伊红染色（H-E染色）和免疫组织化学染色效果。结果  EDTA组脱钙速度较慢，但显示瓣膜组织结构完整，形态清晰，脱钙及免疫组织化学染色效果最佳。硝酸组脱钙时间最短，也能获得良好的脱钙效果。甲酸组脱钙时间较短，但脱钙效果最差。结论  在钙化性主动脉瓣的病理学研究中，EDTA脱钙液仍是脱钙的最佳选择。而在考虑时间因素的情况下，硝酸脱钙液则是良好的替代。甲酸脱钙液由于对钙化瓣膜的脱钙效果较差，不推荐使用。

关键词: 钙化性主动脉瓣, 脱钙液, 免疫组织化学染色

Abstract:

Objective  To compare the effects of three decalcification agents on calcific aortic valves. Methods  Samples of calcific aortic valves of six patients were harvested. Each valve was equally divided into three parts and decalcified by nitric acid of 5% (nitric acid group), formic acid of 10% (formic acid group), and EDTA of 10% (EDTA group), respectively. Pathological sections of three groups were observed and compared after H-E staining and immunohistochemical staining. Results  Although the decalcification time of EDTA group is the longest, but the morphological structure of valve tissues was complete and clear and the effects of decalcification and immunohistochemical staining were the best among three groups. The decalcification time of nitric acid group was the shortest, while the effect of decalcification was satisfactory. The decalcification time of formic acid group was short, but the effect of decalcification was the worst among three groups. Conclusion  EDTA is the best decalcification agent for pathological study on calcific aortic valves. When considering the time factor, nitric acid is a good alternative. Formic acid is not recommended due to the poor decalcified effect on calcific aortic valves.

Key words: calcific aortic valves, decalcification agents, immunohistochemical staining