上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） •    下一篇

幼猪主动脉缩窄模型的术式探讨

马周瑞，于云鹏，蒲 田，白 凯，陈 笋，孙 锟   

  1. 上海交通大学 医学院附属新华医院小儿心脏中心， 上海 200092
  • 出版日期:2013-11-28 发布日期:2013-12-03
  • 通讯作者: 孙 锟, 电子信箱: sunkun@xinhuamed.com.cn。
  • 作者简介:马周瑞（1984—）， 男， 主治医师， 博士生; 电子信箱: zr.ma@pedsheart.net。
  • 基金资助:

    国家自然科学基金（81070134）；国家重点基础研究发展计划（2010CB529500）；上海市高校一流学科B类

Surgery procedures of piglet model of aortic coarctation

MA Zhou-rui, YU Yun-peng, PU Tian, BAI Kai, CHEN Sun, SUN Kun   

  1. Department of Pediatric Heart Center, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China
  • Online:2013-11-28 Published:2013-12-03
  • Supported by:

    National Natural Science Foundation of China, 81070134; National Basic Research Program of China, “973” Program, 2010CB529500; Top Discipline B Program at Shanghai Institutions of Higher Learning

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摘要：

目的 比较环扎法和横切纵缝法建立主动脉缩窄模型的长期效果。方法 分别采用环扎法（环扎组，n=20）和横切纵缝法（横切纵缝组, n=20）建立幼猪主动脉缩窄模型，建模后第1、3、6个月分别进行血管造影检查和病理学检查。另选取10只幼猪作为假手术组。结果 建模后1个月，环扎组和横切纵缝组狭窄段血管直径均显著小于假手术组（P<0.01），狭窄段存在一定的压力阶差。建模后3、6个月，环扎组狭窄段血管直径逐渐增加，与假手术组差异无统计学意义，压力阶差为0；横切纵缝组狭窄段血管直径均显著小于假手术组（P<0.01），压力阶差相对稳定。结论 横切纵缝法可建立长期稳定的主动脉缩窄模型，可作为心血管支架研究的可靠动物模型。

关键词: 主动脉缩窄, 手术方式, 动物模型

Abstract:

Objective To compare long-term effects between banding method (Bme) and transverse aortic incision with longitudinal suture method (TLme) in establishment of experimental aortic coarctation models. Methods Forty piglets were randomly divided into Bme group and TLme group (20 in each) to establish aortic coarctation models. Angiographic and histological examinations were carried out 1, 3, and 6 months after model establishment. Another 10 piglets were served as shamoperation group (S group). Results The stenotic vessel models were established successfully. Compared to S group, the diameters of stenotic vessels were significantly smaller in Bme and TLme groups in 1 month (P<0.01), which existed pressure gradients. But the diameter of stenotic vessels in Bme group gradually increased in time of 3 and 6 months and pressure gradients disappeared, which had no significant differences with S group. However, in TLme group, both diameter of stenotic vessels and pressure gradients maintained the same (P<0.01). Conclusion TLme is an effective surgery procedure to establish experimental coarctation model with long-term stability, and the model may be ideal for the study of cardiovascular stents.

Key words: aortic coarctation, surgery procedure, animal model