上海交通大学学报(医学版)

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雌激素受体α异构体在子宫内膜癌组织中表达及与肿瘤预后的相关性分析

欧阳一芹1,杨 敏2,李 莉1,李怀芳1,童晓文1,高 阗1,朱新贤1   

  1. 1.同济大学 附属同济医院妇产科, 上海 200065; 2.同济大学 医学院, 上海 200092
  • 出版日期:2014-03-28 发布日期:2014-04-02
  • 通讯作者: 朱新贤, 电子信箱: 13817869652@163.com。
  • 作者简介:欧阳一芹(1980—), 女, 住院医师, 博士; 电子信箱: oyyq1124@hotmail.com。
  • 基金资助:

    国家自然科学基金(81101967)

Relationship between expression of estrogen receptor α isoforms in endometrial cancer and its prognosis

OUYANG Yi-qin1, YANG Min2, LI Li1, LI Huai-fang1, TONG Xiao-wen1, GAO Tian1, ZHU Xin-xian1   

  1. 1.Depatment of  Gynecology and  Obstetrics, Tongji Hospital, Tongji University, Shanghai 200065, China; 2.Tongji University School of Medicine, Shanghai 200092, China
  • Online:2014-03-28 Published:2014-04-02
  • Supported by:

    National Natural Science Foundation of China, 81101967

摘要:

目的 探讨雌激素受体α (ERα)不同异构体在子宫内膜癌(EC)中的生物学作用及对EC患者预后的影响。方法 对116例雌激素依赖型EC患者进行回顾性研究,分析EC组织中ERα表达谱与肿瘤临床病理指标、预后的相关性。采用Real-Time PCR检测患者肿瘤组织石蜡切片中RNA的目标基因表达量。结果 ERα表达与EC的分化程度(rs=-0.214, Spearman's test)、淋巴转移(rs=-0.186, Spearman's test)及国际妇产科联盟(FIGO)分期(rs=-0.221, Spearman's test)相关。ERα7号外显子缺失表型(Δ7)是表达量最多的一种异构体,其表达量与肿瘤的分化及FIGO分期相关,且Δ7表达量高者较表达量低者预后佳(疾病特异性生存率P=0.026,疾病无进展生存率P=0.038)。结论 ERαΔ7异构体的表达是决定EC预后的独立危险因子,影响肿瘤患者预后。

关键词: 子宫内膜癌, 雌激素受体, 预后

Abstract:

Objective To explore the impacts of different estrogen receptor α (ERα) isoforms on biological effects of endometrial cancer (EC) and the prognosis of EC patients. Methods A retrospective study was carried out for 116 endometrial cancer patients and an analysis of relationship between the ERα isoform expression pattern of EC tissue and the clinicopathological data and prognosis was performed. Patients' RNAs were extracted from paraffin sections and Real-Time PCR was used to analyze the gene's expression level. Results ERα expression level was related to the tumor differentiation grade (rs=-0.214, Spearman's test), lymphonode metastases (rs=-0.186, Spearman's test), and FIGO stage (rs=-0.221, Spearman's test). ERα exon 7 skipping isoform (Δ7) was the most commonly detected isoform.  Δ7 was related to the tumor differentiation and FIGO stage and its high expression level was found relating to the preferred surviva (diseasespecific survival P=0.026, disease-progression free survival P=0.038). Conclusion ERαΔ7 is an independent prognosis factor of endometrial cancer and its expression level affects the prognosis of endometrial cancer patients.

Key words: endometrial carcinoma, estrogen receptor, prognosis