上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（临床研究） • 上一篇    下一篇

Notch1和FSCN1在乳腺癌中的表达及其临床意义

徐五琴1,2，武世伍1，马 莉1，陶仪声1   

  1. 1．蚌埠医学院附属第一医院病理科， 蚌埠 233030； 2．皖南医学院弋矶山医院病理科， 芜湖 241001
  • 出版日期:2014-09-28 发布日期:2014-09-26
  • 通讯作者: 陶仪声, 电子信箱: tys101041@126.com。
  • 作者简介:徐五琴(1989—)， 女， 硕士生; 电子信箱: 544631369@qq.com。
  • 基金资助:

    安徽省蚌埠医学院研究生科研创新计划项目（Byycx1305）

Expressions of Notch1 and FSCN1 in breast cancer and their clinical significances

XU Wu-qin1,2, WU Shi-wu1, MA Li1, TAO Yi-sheng1   

  1. 1.Department of Pathology， the First Affiliated Hospital， Bengbu Medical College, Bengbu 233030; 2.Department of Pathology， Yijishan Hospital， Wannan Medical College, Wuhu 241001, China
  • Online:2014-09-28 Published:2014-09-26
  • Supported by:

    Graduate Student Research Innovation Project of Bengbu Medical College, Byycx1305

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摘要：

目的 检测Notch1和FSCN1在乳腺癌中的表达及与乳腺癌临床病理特征的关系。方法 应用免疫组织化学方法检测100例乳腺癌组织及30例癌旁正常乳腺组织中Notch1、FSCN1、雌激素受体（ER）、孕激素受体（PR）、CerbB-2及ki-67的蛋白表达；采用RT-PCR检测60例新鲜乳腺癌组织及其癌旁组织中Notch1和FSCN1的mRNA表达。结果 100例乳腺癌组织及30例癌旁正常乳腺组织中Notch1阳性表达率为79.0%（79/100）和33.3%（10/30），FSCN1阳性表达率分别为68.0%（68/100）和30.0%（9/30），两者乳腺癌组织中的阳性率均明显高于癌旁正常乳腺组织（P<0.05）。乳腺癌中Notch1的阳性表达与年龄、ER及PR无明显相关性(P>0.05)，而与腋窝淋巴结转移、临床TNM分期、病理学分级、CerbB-2及ki-67具有相关性（P<0.05）；乳腺癌中FSCN1的阳性表达与年龄无相关性(P>0.05)，而与腋窝淋巴结转移、临床TNM分期、病理学分级、ER、PR、CerbB2及ki-67具有相关性（P<0.05）；Notch1和FSCN1的蛋白表达呈正相关（r=0.225，P<0.05）。RT-PCR结果显示：乳腺癌组织Notch1和FSCN1的表达亦均高于癌旁正常乳腺组织，差异具有统计学意义（P<0.05），Notch1和FSCN1的mRNA表达呈正相关（r=0.446，P<0.05）。结论Notch1和FSCN1在乳腺癌中均呈高表达，可能协同促进乳腺癌的发生和发展，且可能在乳腺癌的浸润、转移及预后中发挥了重要作用。

关键词: 乳腺癌, Notch1, FSCN1, 预后

Abstract:

Objective To detect the expressions in Notch1 and FSCN1 in breast cancer and their relationship with the clinical pathological features of breast cancer. Methods The protein expressions of Notch1, FSCN1, estrogen receptor (ER), progesterone receptor (PR), CerbB-2, and ki-67 of 100 breast cancer tissue samples and 30 adjacent normal breast tissue samples were detected by the immunohistochemical method. The mRNA expressions of Notch1 and FSCN1 of 60 fresh breast cancer tissue samples and corresponding adjacent tissue samples were detected by the RT-PCR. Results For 100 breast cancer tissue samples and 30 adjacent normal breast tissue samples, the positive rates of Notch1 were 79.0% (79/100) and 33.3% (10/30) and the positive rates of FSCN1 were 68.0% (68/100) and 30.0% (9/30). The positive rates of Notch1 and FSCN1 of breast cancer tissue samples were significantly higher than those of adjacent normal breast tissue samples (P<0.05). The positive expression of Notch1 of breast cancer was not significantly relevant to age, ER, and PR (P>0.05), but was relevant to axillary lymph node metastasis, clinical TNM stage, pathological classification, CerbB-2, and ki-67 (P<0.05). The positive expression of FSCN1 of breast cancer was not relevant to age (P>0.05), but was relevant to axillary lymph node metastasis, clinical TNM stage, pathological classification, ER, PR, CerbB-2, and ki-67 (P<0.05). The protein expressions of Notch1 and FSCN1 were positively related (r=0.225, P<0.05). The results of RT-PCR showed that the expressions of Notch1 and FSCN1 of breast cancer tissue were higher than those of adjacent normal breast cancer tissue and the differences were statistically significant (P<0.05). The mRNA expressions of Notch1 and FSCN1 were positively related (r=0.446, P<0.05). Conclusion The expressions of Notch1 and FSCN1 of breast cancer are high and may promote the occurrence and development of breast cancer and play an important role in the invasion, metastasis, and prognosis of breast cancer.

Key words: breast cancer, Notch1, FSCN1, prognosis