上海交通大学学报(医学版)

• 论著(基础研究) • 上一篇    下一篇

绿茶多酚抑制鸡胚绒毛尿囊膜血管生成作用研究

刘 畅1,沈秀华1,唐文静1,谷祖兴2,蔡 威1   

  1. 1.上海交通大学 医学院附属新华医院营养系, 上海 200092; 2.南开大学软件学院, 天津 300071
  • 出版日期:2015-01-28 发布日期:2015-01-29
  • 通讯作者: 沈秀华, 电子信箱: srachel@126.com。
  • 作者简介:刘 畅(1991—), 女, 硕士生; 电子信箱: liuchang14fall@gmail.com。
  • 基金资助:

    国家自然科学基金(81102123);上海市级大学生创新训练项目(IAP6125)

Inhibition of green tea polyphenol on angiogenesis of chick chorioallantoic membrance

LIU Chang1, SHEN Xiu-hua1, TANG Wen-jing1, GU Zu-xing2, CAI Wei1   

  1. 1.Department of Nutrition, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 2.College of Software, Nankai University, Tianjin 300071, China
  • Online:2015-01-28 Published:2015-01-29
  • Supported by:
    National Natural Science Foundation of China, 81102123;Shanghai Undergraduate Innovation Training Program, IAP6125

摘要:

目的 观察绿茶多酚提取物儿茶素(EGCG)抗新生血管形成作用。方法 新鲜种鸡蛋孵化5 d后选择发育正常鸡胚随机分为空白对照组、PBS组、低剂量EGCG组、中剂量EGCG组和高剂量EGCG组,每组各7枚。通过气室分别向各组加入10 μL样品(低、中和高剂量EGCG组分别为100、1 000和10 000 μg/mL的EGCG溶液)。2 d后固定并剪下鸡胚绒毛尿囊膜(CAM),拍照并记录各组CAM血管生长情况,人工计数血管交叉点(VN),计算机扫描并分析血管生成面积(VA)与CAM面积并计算两者之比(VA/CAM)。结果 空白对照组和PBS组鸡胚CAM血管多而粗,EGCG组随着EGCG剂量增加,血管逐渐稀疏分散。1 cm×1 cm面积内各组VN值分别为PBS组(9.5±1.29)个,低剂量EGCG组(9.8±1.30)个,中剂量EGCG组(8.2±1.30)个,高剂量EGCG组(6.5±0.58)个。高剂量EGCG组与其他各组差异有统计学意义(P<0.05)。各组VA/CAM值分别为PBS组0.24±0.04,低剂量EGCG组0.18±0.01,中剂量EGCG组0.14±0.02,高剂量EGCG组0.08±0.01。各组间差异均有统计学意义(P<0.01)。结论 绿茶多酚可抑制CAM新生血管生长,并具有量效关系。

关键词: 绿茶多酚, 儿茶素, 血管生成, 鸡胚绒毛尿囊膜

Abstract:

Objective To observe the effect of green tea polyphenol extract, (-)-epigallocatechin-3-gallate (EGCG), on anti-angiogenesis. Methods Fresh chicken eggs with normal chicken embryos were randomly divided into the blank control group, PBS group, low dose EGCG group, medium dose EGCG group, and high dose EGCG group after being incubated for 5 d and each group consisted of 7 eggs. Then, 10 μL sample was injected through the air chamber of eggs (EGCG solutions of 100, 1 000, and 10 000 μg/mL were used for the low dose, medium dose, and high dose EGCG groups, respectively). Chick chorioallantoic membrane (CAM) was solidified and cut out after 2 d. The angiogenesis of CAM of each group was photographed recorded and the vascular intersection number (VN) was manually counted. The vessel angiogenesis area (VA) was scanned and analyzed and the ratio of VA and CAM (VA/CAM) was calculated. Results Blood vessels of CAM of the blank control group and PBS group were abundant and thick. With the increase of EGCG concentration, blood vessels were gradually sparse and scattered. VNs of 1 cm×1 cm area of the PBS group, low dose EGCG group, medium dose EGCG group, and high dose EGCG group were 9.5±1.29, 9.8±1.30, 8.2±1.30, and 6.5±0.58, respectively. The differences of the high dose EGCG group and other groups were statistically significant (P<0.05). VA/CAM values of the PBS group, low dose EGCG group, medium dose EGCG group, and high dose EGCG group were 0.24±0.04, 0.18±0.01, 0.14±0.02, and 0.08±0.01, respectively. The differences among groups were statistically significant (P<0.01). Conclusion Green tea polyphenol can inhibit the angiogenesis of CAM with dose-dependent effect.

Key words: green tea polyphenol, (-)-epigallocatechin gallate, angiogenesis, chick chorioallantoic membrane