上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（临床研究） • 上一篇    下一篇

Pokemon在胃癌组织中的表达及其临床意义

霍华1，陈红星2，王萍3   

  1. 东明县人民医院 1.肿瘤内科， 2.儿科， 菏泽 274000； 3.上海交通大学 医学院附属第九人民医院病理科， 上海 200011
  • 出版日期:2015-03-28 发布日期:2015-03-26
  • 通讯作者: 王萍, 电子信箱: Wangping897@163.com。
  • 作者简介:霍华（1985—）， 女， 硕士生; 电子信箱: huohualucky@163.com。

Expression and clinical significance of Pokemon in gastric carcinoma tissue

HUO Hua1, CHEN Hong-xing2, WANG Ping3   

  1. 1.Department of Oncology, 2.Department of Pediatrics, Dongming People's Hospital, Heze 274000, China; 3.Department of Pathology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
  • Online:2015-03-28 Published:2015-03-26

PDF (PC)

1191

摘要：

目的 检测Pokemon基因在胃癌组织和癌旁正常胃黏膜组织中的表达水平，并分析其与临床病理特征之间的关系。方法 采用免疫组织化学Eli Vision法检测75例胃癌组织和35例癌旁正常胃黏膜组织中Pokemon蛋白的表达。应用反转录-聚合酶链反应（RT-PCR）技术检测30例新鲜胃癌组织及相应癌旁正常胃黏膜组织中Pokemon mRNA的表达。结果 免疫组织化学分析结果显示，在胃癌和癌旁正常胃黏膜组织中，Pokemon蛋白的阳性表达率分别为78.7% (59/75)和34.3% (12/35)，差异具有统计学意义(P<0.05)；RT-PCR结果显示，30例新鲜胃癌组织中Pokemon mRNA的表达水平显著高于相应癌旁正常胃黏膜组织，差异有统计学意义(P<0.05)；胃癌组织中Pokemon的表达与浸润深度、临床分期和淋巴结转移有关(P<0.05)。结论 Pokemon在胃癌的发生、发展和侵袭转移中具有重要作用，可能成为胃癌治疗的有效靶点。

关键词: Pokemon, 胃癌, 反转录-聚合酶链反应, 免疫组织化学

Abstract:

Objective To detect the expression of Pokemon gene in gastric carcinoma tissue and adjacent normal gastric mucosa tissue and analyze the relationship of the expression of Pokemon gene and cinlicopathologic features. Methods Immunohistochemical staining with Eli Vision method was adopted to detect the expression of Pokemon protein in gastric carcinoma tissue of 75 cases and adjacent normal gastric mucosa tissue of 35 cases. The mRNA expression of Pokemon in fresh gastric cancer tissue and adjacent normal gastric mucosa tissue of 30 cases was detected by the reverse transcriptase-polymerase chain reaction (RT-PCR). Results The results of immunohistochemical analysis showed that the positive expression rates of Pokemon protein in gastric cancer tissue and adjacent normal gastric mucosa tissue were 78.7% (59/75) and 34.3% (12/35), respectively. The difference was statistically significant (P<0.05). The results of RT-PCR showed that the mRNA expression levels of Pokemon in fresh gastric cancer tissue of 30 cases were significantly higher than those of adjacent normal gastric mucosa tissue. The differences were statistically significant (P<0.05). The expression of Pokemon in gastric carcinoma tissue was relevant to infiltration depth, clinical stages, and lymph node metastasis (P<0.05). Conclusion The expression of Pokemon plays an important role in the occurrence, development, invasion, and metastasis of gastric carcinoma. Pokemon may become an effective target for the theatment of gastric cancer.

Key words: Pokemon, gastric carcinoma, reverse transcriptase-polymerase chain reaction, immunohistochemistry