上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

干细胞核心基因NANOG在乳腺癌干细胞及非干细胞中的表达与定位

张志影1,2，徐亮3，徐迎春4，徐海燕2，张凤春2,3   

  1. 1.徐州医学院研究生院， 徐州 221000； 2.上海交通大学 医学院附属苏州九龙医院肿瘤科， 苏州 215021； 3.上海交通大学 医学院附属瑞金医院肿瘤科， 上海 200025； 4.上海交通大学 医学院附属仁济医院肿瘤科， 上海 200001
  • 出版日期:2015-07-28 发布日期:2015-08-27
  • 通讯作者: 张凤春, 电子信箱: fczhang2004@163.com。
  • 作者简介:张志影（1988—）， 女， 硕士生; 电子信箱: zhangzhiying_223@163.com。
  • 基金资助:

    国家自然科学基金（81172522, 81301858）；苏州市科技局科研课题（SYS201404）

Expression and location of core gene NANOG of stem cells in breast cancer stem cells and non-breast cancer stem cells

ZHANG Zhi-ying1,2, XU Liang3, XU Ying-chun4, XU Hai-yan2, ZHANG Feng-chun2,3   

  1. 1.Graduate School, Xuzhou Medical College, Xuzhou 221000, China; 2.Department of Oncology, Suzhou Kowloon Hospital, Shanghai Jiao Tong University School of Medicine, Suzhou 215021, China; 3.Department of Oncology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 4.Department of Oncology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200001, China
  • Online:2015-07-28 Published:2015-08-27
  • Supported by:

    National Natural Science Foundation of China, 81172522, 81301858; Research Projects of Suzhou Municipal Science and Technology Bureau, SYS201404

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摘要：

目的 研究干细胞核心基因NANOG在乳腺癌干细胞（BCSCs）和非乳腺癌干细胞（Non-BCSCs）中的表达及定位差异以及BCSCs在MCF-7微球体中的分布情况。方法 应用免疫荧光、免疫细胞化学及NANOG与Hoechst33342荧光染料双染色方法检测BCSCs和NonBCSCs中NANOG的表达及其定位。结果 免疫细胞化学及免疫荧光染色显示，MCF-7中存在两种细胞：一种细胞胞体大、细胞质丰富，NANOG主要表达其细胞质中，这部分细胞占绝大多数；另一种细胞胞体小而圆、细胞质少，NANOG主要表达其细胞核中，这部分细胞占少数。NANOG和Hoechst33342免疫荧光双染显示：NANOG表达于Hoechst33342拒染的细胞核中；而在Hoechst33342核染色的细胞中，NANOG主要表达于细胞质中。微球体呈球囊性、中心部呈空腔状，细胞沿着球壁分布，球壁多为1~3层细胞组成；在MCF-7微球体中，BCSCs主要分布在囊性微球体的内侧壁。结论 NANOG在BCSCs中主要表达在细胞核，而在Non-BCSCs中主要表达在细胞质，而BCSCs主要分布在囊性微球体的内侧壁，这可为BCSCs的进一步研究和乳腺癌的临床靶向治疗提供实验依据。

关键词: NANOG, BCSCs, 微球体, 亚细胞定位

Abstract:

Objective To explore the expression and location differences of core gene NANOG of stem cells in breast cancer stem cells (BCSCs) and non-breast cancer stem cells (Non-BCSCs) as well as the distribution of BCSCs in mammospheres of MCF-7. Methods The expression and localization of NANOG in BCSCs and NonBCSCs were detected by immunohistochemistry, immunocytochemistry, and double staining of NANOG and flourescent dye Hoechst33342. Results Immunocytochemistry and immunofluorescence staining showed that there were two types of cells in MCF-7. Most cells had big bodies and abundant cytoplasm, and NANOG mainly expressed in the cytoplasm. Other cells had small round bodies and less cytoplasm, and NANOG mainly expressed in the nucleus. NANOG and Hoechst33342 immunofluorescence double staining showed that NANOG expressed in the nucleus that could not be stained by Hoechst33342. In cells that the nucleus was stained by Hoechst33342, NANOG mainly expressed in the cytoplasm. The mammospheres were cystic sphere and hollow in center. Cells distributed along the wall of the mammosphere and the wall mainly consisted of 1 to 3 layers of cells. BCSCs mainly distributed in the inner layer of MCF-7 cystic mammospheres. Conclusion NANOG mainly express in the nucleus of BCSCs and the cytoplasm of Non-BCSCs. BCSCs mainly distribute in the inner layer of the cystic mammospheres, which provides the experimental evidence for further research of BCSCs and clinical target therapy of breast cancer.

Key words: NANOG, BCSCs, mammosphere, subcellular localization