上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

Millar压力容积导管与超声心动图联合评价骨髓单个核细胞移植对心肌梗死小鼠的治疗效果

张文天，陈瑶，连锋，薛松   

  1. 上海交通大学  医学院附属仁济医院心血管外科, 上海 200127
  • 出版日期:2015-07-28 发布日期:2015-08-27
  • 通讯作者: 连锋, 电子信箱: dr.lianfeng@hotmail.com。
  • 作者简介:张文天（1988—）， 男， 硕士生; 电子信箱: zwtstzjz@163.com。
  • 基金资助:

    上海市科委基金（12nm0502100）；上海市教委基金（11YZ48， 13ZZ091）；上海市科委医学引导项目（124119a2100）；上海交通大学多学科交叉项目培育（医工）（YG2013MS38）

Evaluation of effect of bone marrow mononuclear cells transplantation on treatment of mice with myocardial infarction by Millar pressure-volume conductance catheter system combined with echocardiography

ZHANG Wen-tian, CHEN Yao, LIAN Feng, XUE Song   

  1. Department of Cardiovascular Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai  200127, China
  • Online:2015-07-28 Published:2015-08-27
  • Supported by:

    Foundation of Science and Technology Commision of Shanghai Municipality,12NM0502100; Foundation of Shanghai Municipal Education Committee, 11YZ48,13ZZ091; Medicine Guidance Project of Science and Technology Commission of Shanghai Municipality，124119a2100； Medicine-Engineering Cross-Research Foundation of Shanghai Jiao Tong University，YG2013MS38

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摘要：

目的  采用Millar压力容积导管和超声心动图研究骨髓单个核细胞（BMMNCs）移植对心肌梗死小鼠的治疗作用。方法  30只小鼠随机分为心肌梗死组、心肌梗死BMMNCs移植治疗组（BMMNCs组）和假手术组，每组10只。心肌梗死组和BMMNCs组小鼠进行心肌梗死造模，4 h后BMMNCs组小鼠经尾静脉注射BMMNCs；7 d后行超声心动图和Millar压力容积导管检测，记录各组小鼠心功能指标的变化；心脏组织切片TTC染色后光学显微镜下观察心肌梗死面积。结果  Millar压力容积导管检测结果显示：与假手术组比较，心肌梗死组小鼠心输出量（CO）、左心室收缩末压（Pes）、等容收缩期左心室内压力上升最大速率（dP/dtmax）、射血分数（LVEF）均显著降低(P<0.05)；而左心室舒张末压（Ped）、等容舒张期左心室压力下降最大速率（dP/dtmin）及左心室心肌松弛时间常数（Tau）均显著上升（P<0.05）。BMMNCs组小鼠CO、Pes、dP/dtmax、EF高于心肌梗死组（P<0.05），而Ped、dP/dtmin和Tau显著低于心肌梗死组（P<0.05）。BMMNCs组小鼠各项指标与假手术组的差异仍有统计学意义（P<0.05）。超声心动图显示：心肌梗死组和BMMNCs组左心室后壁的增厚情况均不明显；但与假手术组相比，两组均出现左心室内径增加及左心室舒张和收缩末期容积增加（P<0.05）。心脏组织切片TTC染色光镜下观察发现：BMMNCs组小鼠心肌梗死面积明显小于心肌梗死组（P<0.05）。结论 Millar压力容积导管与超声心动图可从多方面监测小鼠心功能，评估BMMNCs移植改善小鼠心肌梗死的情况，对研究心肌梗死后小鼠心功能变化具有重要意义。

关键词: Millar压力容积导管, 超声心动图, 心肌梗死, 骨髓单个核细胞移植

Abstract:

Objective   To explore the effect of bone marrow mononuclear cells (BMMNCs) transplantation on the treatment of mice with myocardial infarction (MI) by Millar pressure-volume conductance catheter system and echocardiography. Methods  A total of 30 mice were randomly divided into the MI group (n=10), MI+BMMNCs treatment group (BMMNCs group, n=10), and sham operation group (n=10). MI model was established for mice of MI and BMMNCs groups. Mice of the BMMNCs group were injected with BMMNCs through caudal vein 4 h after MI model was established. Echocardiography test and Millar pressure-volume conductance catheter test were performed 7 d after MI model was established. Changes of cardiac function indexes of each group were recorded. Slices of cardiac tissue were stained by TTC and the area of MI was observed under optical microscope. Results  Results of Millar pressure-volume conductance catheter test showed that compared with the sham operation group, the cardiac output (CO), left ventricular end-systolic pressure (Pes), dP/dtmax, and left ventricular ejection fraction (LVEF) of the MI group remarkably decreased (P<0.05), while left ventricular end-diastolic pressure (Ped), dP/dtmin, and constant of isovolumetric relaxation (Tau) remarkably increased (P<0.05). CO, Pes, dP/dtmax, and EF of the BMMNCs group were higher than those of the MI group (P<0.05), while Ped, dP/dtmin, and Tau were significantly lower than those of the MI group (P<0.05). The differences of indexes between BMMNCs group and sham operation group were statistically significant (P<0.05). Echocardiography showed that interventricular septum of the MI group and BMMNCs group did not remarkably thicken. But compared with the sham operation group, the left ventricular diameter (LVD) and left ventricular end-diastolic/systolic volume (LVED, LVES) of the MI group and BMMNCs group increased (P<0.05). The observation of TTC stained cardiac tissue slices under optical microscope showed that the area of MI of the MMNCs group was significantly smaller than that of the MI group (P<0.05). Conclusion  Millar pressure-volume conductance catheter and echocardiography can monitor the cardiac function of mice from many aspects, evaluate the improvement of cardiac function by BMMNCs transplantation, and are significant for investigating the changes of cardiac function of mice after the incidence of MI.

Key words: Millar pressure-volume conductance catheter, echocardiography, myocardial infarction, bone marrow mononuclear cell transplantation