上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

七氟醚麻醉对老年小鼠空间认知能力以及海马组织pCreb和Egr1表达的影响

周国霞，朋立超   

  1. ZHOU Guo-xia, PENG Li-chao
  • 出版日期:2015-08-28 发布日期:2015-09-30
  • 通讯作者: 朋立超, 电子信箱: plichao@foxmail.com。
  • 作者简介:周国霞（1977—）， 女， 副主任医师， 硕士生; 电子信箱: zgx6742997@163.com。

Effects of sevoflurane anesthesia on spatial recognition and expressions of pCreb and Egr1 in hippocampus tissues of aged mice

复旦大学附属上海市第五人民医院麻醉科， 上海 200240   

  1. Department of Anesthesia, Shanghai Fifth People's Hospital, Fudan University, Shanghai200240, China
  • Online:2015-08-28 Published:2015-09-30

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摘要：

目的  探讨七氟醚麻醉对老年小鼠空间认知能力以及海马组织中磷酸化环腺苷酸应答元件结合蛋白（pCreb）和早期生长反应因子1（Egr1）表达的影响。方法  将12只18月龄老年小鼠随机分为麻醉组和对照组，每组各6只。麻醉组小鼠吸入3%七氟醚4 h，而对照组小鼠吸入100%氧气4 h。水迷宫空间学习记忆能力测量在麻醉后48 h进行，获得训练结束后24 h进行空间探索试验。空间探索试验结束后15 min分离海马组织，通过Western blotting和实时定量PCR方法检测海马组织Egr1、Creb和pCreb的表达。结果  麻醉组小鼠第4、5、6日逃逸潜伏期显著长于对照组（P<0.05）；在空间探索试验中，对照组平台象限时间、路程百分比显著高于麻醉组（P<0.01）。与对照组比较，麻醉组小鼠海马组织Egr1的表达量显著下降（P<0.05）；而两组Creb的表达量无显著差异（P>0.05），但麻醉组磷酸化水平下降（P<0.05）。结论  七氟醚麻醉下调海马组织Egr1表达和Creb磷酸化水平，影响老年小鼠空间认知能力。

关键词: 海马, 环腺苷酸应答元件结合蛋白, 早期生长反应因子1, 空间探索试验, 老年小鼠, 七氟醚

Abstract:

Objective  To explore the effects of sevoflurane anesthesia on spatial recognition and expressions of pCreb and early growth response factor 1 (Egr1) in hippocampus tissues of aged mice. Methods  A total of 12 Eighteen-month-old mice were randomly divided into the anesthesia group (n=6) and control group (n=6). Mice of the anesthesia group and control group inhaled 3% sevoflurane and 100% oxygen for 4 h, respectively. Spatial learning and memory abilities were tested by the Morris water maze 48 h after anesthesia. The spatial probe test was conducted 24 h after memory acquisition test. Hippocampus tissues were harvested 15 min after the spatial probe test and expressions of Egr1, Creb, and pCreb in hippocampus tissues were detected by Western blotting and RT-qPCR. Results  The escape latency of anesthesia group was significantly longer than that of the control group on day 4, 5, and 6 (P<0.05). The time spent in the target quadrant of the control group was significantly longer than that of the anesthesia group and the percentage of distance of the control group was significantly higher than that of the anesthesia group during the spatial probe test (P<0.01). Compared with the control group, the expression of Egr1 in hippocampus tissues of the anesthesia group remarkably decreased (P<0.05) and the expression of pCreb decreased (P<0.05). The difference of the expression of Creb between two groups was not significant (P>0.05). Conclusion  Sevoflurane down-regulates expressions of pCreb and Egr1 in hippocampus tissues and affects the spatial recognition of aged mice.

Key words: hippocampus, cAMP response element binding protein, early growth response protein 1, spatial probe test, aged mouse, sevoflurane