上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（临床研究） • 上一篇    下一篇

肾胺酶基因rs2576178变异与2型糖尿病肾病患者高血压发病的关系

张荣1，葛晓旭1，王锋2，庄兰艮1，赵蔚菁1，李鸣1，郑泰山1，汪年松2，刘丽梅1   

  1. 上海交通大学附属第六人民医院 1.内分泌代谢科 上海市糖尿病研究所, 2.肾内科， 上海 200233
  • 出版日期:2015-08-28 发布日期:2015-09-30
  • 通讯作者: 刘丽梅, 电子信箱: lmliu@sjtu.edu.cn。
  • 作者简介:张荣（1988—）， 女， 硕士生; 电子信箱: zhang_rong0428@126.com。
  • 基金资助:

    国家自然科学基金(81471012, 81270876, 30771022, 30971384);上海市科委优秀学科带头人基金(10XD1403400);上海市交通大学医学院教育研究课题(YB150612)

Relationship between rs2576178 mutation of renalase gene and incidence of hypertension of type 2 diabetic nephropathy patients

ZHANG Rong1, GE Xiao-xu1, WANG Feng2, ZHUANG Lan-gen1, ZHAO Wei-jing1, LI Ming1， ZHENG Tai-shan1， WANG Nian-song2， LIU Li-mei1   

  1. 1.Shanghai Diabetes Institute, Department of Endocrinology and Metabolism, 2.Department of Nephrology, Shanghai Sixth Peoples Hospital, Shanghai Jiao Tong University, Shanghai 200233, China
  • Online:2015-08-28 Published:2015-09-30
  • Supported by:

    National Natural Science Foundation of China, 81471012；81270876；30771022； 30971384； Program of Shanghai Subject Chief Scientist, 10XD1403400; Program of Education Research from Shanghai Jiaotong University School of Medicine, YB150612

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摘要：

目的  探讨肾胺酶基因（RNLS）rs2576178变异与合并微量白蛋白尿的非胰岛素依赖型糖尿病（2型糖尿病）肾病患者高血压发病的关系。方法  以上海地区225例（均为汉族）合并微量白蛋白尿的2型糖尿病肾病患者（DN组）和251例无糖尿病、无肾脏疾病的健康对照者（Cont组）作为研究对象，DN组又再分为合并高血压组[DN-HTN（+）组]169例和未合并高血压组[DN-HTN（-）]56例进行分析。采用Taqman分型方法，检测以上研究对象RNLS基因rs2576178位点的基因分型，比较两组间基因型频率、等位基因频率分布及临床变量间的差异。结果  DN组和Cont组rs2576178基因型频率分布符合Hardy-Weinberg平衡定律。与Cont组相比，DN组AA基因型和A等位基因频率显著降低（P<0.05）；应用非条件Logistic回归分析对所有研究对象的性别、年龄、体质量指数（BMI）进行校正后，rs2576178 AA基因型仍与高血压显著相关，OR（95%CI）为0.48（0.28～0.81）。与DN-HTN（－）组相比，DN-HTN（+）组GG+GA基因型和G等位基因频率显著增加（P<0.05）。对DN组性别、发病年龄、BMI进行校正后，rs2576178 AA基因型亦与高血压显著相关，OR（95%CI）为0.37（0.17～0.79）。结论  RNLS基因rs2576178 AA基因型可能具有防止合并微量白蛋白尿的2型糖尿病肾病患者高血压发生的作用。

关键词: 肾胺酶基因, rs2576178变异, 非胰岛素依赖型糖尿病, 微量白蛋白尿, 高血压

Abstract:

Objective  To investigate the relationship between rs2576178 mutation of renalase gene (RNLS) and the incidence of hypertension of type 2 diabetic nephropathy patients with micro-albuminuria. Methods  A total of 225 type 2 diabetic nephropathy patients with micro-albuminuria (DN group, all Han nationality) and 251 healthy controls (control group) were enrolled. The DN group was further divided into the DN-HTN(+) group (combined with hypertension, n=169) and DN-HTN(-) group (not combined with hypertension, n=56). The genotype of locus rs2576178 of RNLS gene of subjects was detected by the Taqman PCR assay. The differences of genotypic and allelic frequencies and clinical variables between two groups were compared. Results  The genotype distribution of rs2576178 of DN group and control group was consistent with Hardy-Weinberg equilibrium. Compared with the control group, AA genotypic and A allelic frequencies of the DN group significantly decreased (P<0.05). AA genotype of rs2576178 significantly correlated with hypertension after sex, age, and BMI of all subjects were corrected by non-conditional Logistic regression analysis with OR of 0.48 (95%CI: 0.28-0.81). Compared with the DN-HTN (-) group, GG+GA genotypic and G allelic frequencies significantly increased in DN-HTN(+) group (P<0.05). AA genotype of rs2576178 significantly correlated with hypertension after sex, age, and BMI of the DN group were corrected with OR of 0.37 (95%CI: 0.17-0.79). Conclusion  AA genotype of rs2576178 of RNLS may decrease the incidence of hypertension of type 2 diabetic nephropathy patients with micro-albuminuria.

Key words: renalase gene, rs2576178 mutation, type 2 diabetic nephropathy, micro-albuminuria, hypertension