上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

灯盏花素对胰岛素抵抗大鼠的影响及其机制研究

赵栋梁1，武莉2，李锦平2，张馨媛1，冯吉波2   

  1. 1.山西医科大学 药理学教研室， 太原 030000； 2.山西医科大学汾阳学院 药理学教研室， 汾阳 032200
  • 出版日期:2016-06-28 发布日期:2016-07-25
  • 通讯作者: 李锦平, 电子信箱: lijp5044@163.com。
  • 作者简介:赵栋梁（1991—）， 女， 硕士生; 电子信箱: zdl1241830627@163.com。
  • 基金资助:

    山西省科技攻关项目（20051903）；山西医科大学汾阳学院科研基金重点项目（1418）

Study on the effects of breviscapine on rats with insulin resistance and related mechanism

ZHAO Dong-liang1， WU Li2， LI Jin-ping2， ZHANG Xing-yuan1， FENG Ji-bo2   

  1. 1.Department of Pharmacology， Shanxi Medical University， Taiyuan 030000， China； 2.Department of Pharmacology， Fenyang College Shanxi Medical University， Fenyang 032200， China
  • Online:2016-06-28 Published:2016-07-25
  • Supported by:

    Key Science and Technology of Shanxi Province， 20051903； Major Program of Scientific Research Fund of Fenyang College Shanxi Medical University， 1418

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摘要：

目的 探讨灯盏花素对胰岛素抵抗（IR）大鼠的影响及其可能的作用机制。方法 50只SD雄性大鼠随机分为正常对照组（NC组，n=10）和IR模型组（n=40）。NC组喂以普通饲料，IR模型组大鼠经高脂高糖饲料喂养12周建立IR模型。筛选30只成模的IR大鼠，随机均分为空白对照模型组（HFD组）、灯盏花素高剂量组（High组）和灯盏花素低剂量组（Low组），每组10只。测定大鼠血清空腹胰岛素（FINS）和空腹血糖值（FBG），计算稳态模型评估的胰岛素抵抗指数（HOMA-IR）；免疫组织化学法及Western blotting检测灯盏花素治疗后IR大鼠肝脏组织中核糖体S6激酶1(S6K1)蛋白及胰岛素受体底物1(IRS-1)酪氨酸磷酸化水平。结果 与NC组相比，HFD组大鼠HOMA-IR明显增高（P=0.000）；与HFD组相比，High组和Low组大鼠HOMA-IR明显降低（均P=0.000）；免疫组织化学法及Western blotting检测结果显示与HFD组相比，High组和Low组大鼠肝脏组织中S6K1蛋白的表达量均明显下调（均P<0.01），IRS-1酪氨酸磷酸化水平均增高（P<0.01, P<0.05）。结论 灯盏花素显著改善IR大鼠的IR状态，其作用可能与大鼠肝脏组织中S6K1的表达下调以及IRS-1酪氨酸磷酸化水平增高有关。

关键词: 灯盏花素, 胰岛素抵抗, 核糖体S6激酶1, 胰岛素受体底物1, 酪氨酸磷酸化

Abstract:

Objective To investigate the effects of breviscapine on rats with insulin resistance (IR) and possible mechanism. Methods Fifty SD male rats were randomly assigned to the normal control group (NC group, n=10, fed with a normal diet) and IR model group (n=40, fed with a high fat and high sugar diet for 12 weeks for establishment of IR model). Thirty rats in IR model group were selected and randomly assigned to blank control model group (HFD group), high-dose breviscapine group (High group), and low-dose breviscapine group (Low group) with 10 rats in each group. The serum fasting insulin (FINS) and fasting blood glucose (FBG) were measured and the homeostasis model assessment of insulin resistance (HOMA-IR) index was calculated. The protein expression of ribosomal protein S6 kinase 1 (S6K1) and phosphorylation of insulin receptor substract-1 (IRS-1) tyrosine in liver tissues of IR rats after breviscapine treatment were detected with Western blotting and immunohistochemistry (IHC). Results The HOMA-IR index in HFD group was significantly higher as compared with NC group (P=0.000). The HOMA-IR indexes in High group and Low group were significantly lower as compared with HFD group (P=0.000). The results of Western blotting and IHC showed that the expression of S6K1 protein in High group and Low group were lower (P<0.01) and the phosphorylation of IRS-1 tyrosine was higher as compared with HFD group (P<0.01, P<0.05). Conclusion Breviscapine can significantly ameliorate insulin resistance in IR rats. The mechanism may be related to the decreased expression of S6K1 and increased phosphorylation of IRS-1 tyrosine in rat liver tissues.

Key words: breviscapine, insulin resistance, ribosomal protein S6 kinase 1, insulin receptor substract-1, tyrosine phosphorylation