上海交通大学学报(医学版) ›› 2020, Vol. 40 ›› Issue (1): 30-.doi: 10.3969/j.issn.1674-8115.2020.01.005

• 论著·基础研究 • 上一篇    下一篇

枸杞多糖和有氧运动对大鼠非酒精性脂肪肝的干预效果及其机制研究

郭怡琼,吴 琼,吴雅婷,高璐璐,杨建军   

  1. 宁夏医科大学公共卫生与管理学院,银川 750004
  • 出版日期:2020-01-28 发布日期:2020-03-05
  • 通讯作者: 杨建军,电子信箱:yangjianjun1970@163.com。
  • 作者简介:郭怡琼(1992—),女,硕士生;电子信箱:gyq904329124@163.com。
  • 基金资助:
    国家自然科学基金(81660537)。

Effect of Lycium barbarum polysaccharide and aerobic exercise on rats with non-alcoholic fatty liver disease and its mechanism

GUO Yi-qiong, WU Qiong, WU Ya-ting, GAO Lu-lu, YANG Jian-jun   

  1. School of Public Health and Management, Ningxia Medical University, Ningxia Hui Autonomous Region, Yinchuan 750004, China
  • Online:2020-01-28 Published:2020-03-05
  • Supported by:
    National Natural Science Foundation of China (81660537).

摘要: 目的·研究枸杞多糖(Lycium barbarum polysaccharides,LBP)、有氧运动对大鼠非酒精性脂肪肝(non-alcoholic fatty liver disease,NAFLD)的干预效果及其作用机制。方法·将8周龄SD大鼠给予高脂饮食(high fat diet,HFD)10周,建立NAFLD模型。造模成功后将其随机分为HFD组(无干预)、低剂量(50 mg/kg)LBP干预组(LBP-a组)、高剂量(100 mg/kg)LBP干预组(LBP-b组)和有氧运动(20 m/min,60 min/d)干预组,每组8只,3个干预组均干预6周。另设对照组,给予正常饮食。干预结束后,禁食12 h,测定大鼠空腹血糖;10%水合氯醛腹腔注射麻醉处死后,取血液样本和肝脏组织。用相应的试剂盒测定大鼠谷丙转氨酶(glutamate pyruvic transaminase,GPT)、谷草转氨酶(glutamic oxaloacetic transaminase,GOT)、总胆固醇(total cholesterol,TC)、三酰甘油(triacylglycerol,TAG)、低密度脂蛋白(low density lipoprotein,LDL)、高密度脂蛋白(high density lipoprotein,HDL)、丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)及irisin水平;反转录聚合酶链反应测定肝脏过氧化物酶体增殖物激活受体γ共激活因子-1α(peroxisome proliferators-activated receptor-γ coactivator-1 α,Pgc1-α)、纤维连接蛋白Ⅲ型结构域5(fibronectin type Ⅲ domain-containing protein 5,Fndc5)基因的表达。结果·与HFD组比较,3个干预组大鼠GPT、GOT、TC、TAG、MDA水平均显著降低(PPPPPgc1-α mRNA的表达显著升高(PFndc5 mRNA的表达均显著升高(P结论·枸杞多糖和有氧运动能够降低NAFLD大鼠的脂代谢紊乱程度,降低肝脏氧化应激水平。这一过程可能与Pgc1-α和Fndc5 mRNA表达上调有关。

关键词: 枸杞多糖, 有氧运动, 高脂饮食, 非酒精性脂肪肝

Abstract:

Objective · To investigate the effects of different doses of Lycium barbarum polysaccharides (LBP) and aerobic exercise on rats with non-alcoholic fatty liver disease (NAFLD) and its mechanisms. Methods · SD rats of 8 weeks old were randomly divided into normal control group and high fat diet (HFD) group. The normal control group was given normal diet, and the HFD group was given HFD for 10 weeks to construct a NAFLD rat model. After successful modeling, the rats with NAFLD were assigned into four groups with eight in each, namely: HFD group, LBP-a group (50 mg/kg), LBP-b group (100 mg/kg) and aerobic exercise group (20 m/min, 60 min/d). After the intervention for 6 weeks, fasting for 12 hour and fasting blood glucose level was measured. Blood samples and liver tissues of rats were collected after intraperitoneal anesthesia with 10% chloral hydrate. The levels of glutamate pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), total cholesterol (TC), triacylglycerol (TAG), low density lipoprotein (LDL), high density lipoprotein (HDL), malondialdehyde (MDA), superoxide dismutase (SOD) and irisin in rats were measured according to corresponding protocols of kits. The s of peroxisome proliferators-activated receptor-γ coactivator-1 α (Pgc1-α) and fibronectin type Ⅲ domain-containing protein 5 (Fndc5) in liver were detectedusing reverse transcription polymerase chain reaction. Results · Compared with the HFD group, the levels of GPT, GOT, TC, TAG, MDA and homeostasis model assessment of insulin resistance (HOMA-IR) were significantly decreased in LBP-a group, LBP-b group and aerobic exercise group (PPPPgc1-α mRNA in LBP-b group and aerobic exercise group were up-regulated as compared with the HFD group (PFndc5 mRNA in three intervention groups were up-regulated as compared with the HFD group (PConclusion · LBP and aerobic exercise can significantly improve lipid metabolism disorder and decrease liver oxidative stress in rats with NAFLD, which may be related to the up-regulated s of Pgc1-α mRNA and Fndc5 mRNA.

Key words: Lycium barbarum polysaccharides , aerobic exercise, high fat diet, non-alcoholic fatty liver disease