上海交通大学学报（医学版）

›› 2009, Vol. 29 ›› Issue (8): 918-.

• 论著（基础研究） • 上一篇    下一篇

不同浓度硝酸甘油对大鼠离体心脏保护8 h的效果

陈 琦, 李洲斌, 项 捷, 臧旺福   

  1. 上海交通大学 医学院瑞金医院心胸外科, 上海 200025
  • 出版日期:2009-08-25 发布日期:2009-09-27
  • 通讯作者: 臧旺福, 电子信箱: zangwf@hotmail.com。
  • 作者简介:陈琦（1982—）, 男, 住院医师, 硕士生；电子信箱: chenqi0525@msn.com。
  • 基金资助:

    教育部高等学校博士点基金（20070248079）

Effects of nitroglycerine of different concentrations on rat cardiac preservation for 8 h

CHEN Qi, LI Zhou-bin, XIANG Jie, ZANG Wang-fu   

  1. Department of Cardiothoracic Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2009-08-25 Published:2009-09-27
  • Supported by:

    Foundation of the Ministry of Education of China, 20070248079

PDF (PC)

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摘要：

目的 探讨在托马斯液中加入不同浓度硝酸甘油对大鼠离体心脏保护8 h的效果。 方法 取30只Wistar大鼠，建立Langendorff离体心脏灌注模型，按保存液不同随机分为单纯托马斯液组、托马斯液+4×10-6 mol/L硝酸甘油组和托马斯液+4×10-5 mol/L硝酸甘油组，每组10只，比较4 ℃低温保存8 h后各组心脏功能指标；切取左心室壁组织于透射电镜下观察超微结构变化。 结果 4 ℃低温保存8 h后，与单纯托马斯液组相比，托马斯液+4×10-5 mol/L硝酸甘油组的心功能显著改善，细胞超微结构受损较轻；而托马斯液+4×10-6 mol/L硝酸甘油组各指标则无显著改善。 结论 在托马斯液中加入4×10-5 mol/L硝酸甘油可显著提高大鼠离体心脏的保存效果。

关键词: 心脏移植, 心肌保护, 硝酸甘油, 一氧化氮

Abstract:

Objective To investigate the effects of Thomas solution in combination with nitroglycerine of different concentrations on rat cardiac preservation for 8 h. Methods Thirty Wistar rats were selected, and Langendorff heart perfusion models were established. Rats were randomly divided into Thomas solution group, Thomas solution+4×10-6 mol/L nitroglycerine group and Thomas solution+4×10-5 mol/L nitroglycerine group (n=10). After hypothermic preservation (4 ℃）for 8 h, the cardiac function of each group was determined. Tissues of left ventricular wall were obtained, and changes of myocardial ultrastructure were observed by electron microscopy. Results Compared with Thomas solution group, the cardiac function was significantly improved and the myocardial ultrastructure injury was less severe in Thomas solution+4×10-5 mol/L nitroglycerine group after hypothermic preservation (4 ℃）for 8 h, while there was no significant difference in myocardial ultrastructure injury and cardiac function between Thomas solution group and Thomas solution+4×10-6 mol/L nitroglycerine group. Conclusion Thomas solution in combination with 4×10-5 mol/L nitroglycerine can significantly improve the preservation effects for isolated hearts in rats.

Key words: heart transplantation, myocardial preservation, nitroglycerine, nitric oxide

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