上海交通大学学报（医学版）

›› 2009, Vol. 29 ›› Issue (9): 1045-.

• 论著（基础研究） • 上一篇    下一篇

牙周炎患者牙龈组织中TLR-2和TLR-4的表达

郭秋曼, 束 蓉   

  1. 上海交通大学 医学院第九人民医院牙周科 上海市口腔医学重点实验室, 上海 200011
  • 出版日期:2009-09-25 发布日期:2009-09-29
  • 通讯作者: 束蓉, 电子信箱: shurong123@hotmail.com。
  • 作者简介:郭秋曼（1982—）, 住院医师, 硕士；电子信箱: jacky_kow@hotmail.com。
  • 基金资助:

    上海市科委基金（08JC1414600, 08DZ2271100)

Expressions of TLR-2 and TRL-4 in gingival tissues of patients with periodontitis

GUO Qiu-man, SHU Rong   

  1. Department of Periodontology, Shanghai Key Laboratory of Stomatology, The Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200011, China
  • Online:2009-09-25 Published:2009-09-29
  • Supported by:

    Shanghai Science and Technology Committee Foundation, 08JC1414600, 08DZ2271100

PDF (PC)

1434

摘要：

目的 研究Toll样受体-2（TLR-2）和TLR-4在不同类型牙周炎及不同炎症程度的牙龈组织中的分布和表达水平，初步探讨TLR-2和TLR-4在牙周炎发生和发展中的可能作用。方法 采集牙龈组织标本分为五组（n=10）：正常对照组、慢性牙周炎炎症组、慢性牙周炎临床相对健康组、侵袭性牙周炎炎症组和侵袭性牙周炎临床相对健康组。免疫组织化学方法检测各组牙龈组织中TLR-2和TLR-4的分布和表达水平。结果 TLR-2和TLR-4在牙龈结缔组织全层中均有表达，TLR-4在牙龈上皮也有表达。与正常对照组比较，其余各组牙龈组织中TLR-2和TLR-4表达均显著增加（P<0.05）。牙周炎炎症组中，慢性牙周炎组TLR-2和TLR-4表达显著高于侵袭性牙周炎组（P<0.05）。牙周炎临床相对健康组中，慢性牙周炎组TLR-4表达显著高于侵袭性牙周炎组（P<0.05），而TLR-2表达显著低于侵袭性牙周炎组（P<0.05）。结论 TLR-2和TLR-4可能参与牙周炎症过程，但在不同类型牙周炎牙龈组织中的表达可能存在差异。

关键词: Toll样受体, 慢性牙周炎, 侵袭性牙周炎, 牙龈

Abstract:

Objective To detect the distributions and expressions of Toll-like receptor-2 (TLR-2) and TLR-4 in different kinds of periodontitis and different extent of the inflammation of gingival tissues and to discuss the roles of TLR-2 and TLR-4 in the progress of periodontal inflammation. Methods Gingival biopsies were divided into 5 groups: control group (n=10), chronic periodontitis group (n=10), chronic periodontitis clinically healthy group (n=10), aggressive periodontitis group (n=10), and aggressive periodontitis clinically healthy group (n=10). The distributions and expressions of TLR-2 and TLR-4 were detected by immunohistochemistry. Results TLR-2 and TLR-4 expressed in all layers of gingival connective tissues. TLR-4 was also observed in gingival epithelium. Compared to control group, expressions of TLR-2 and TLR-4 were significantly higher than those in the other 4 groups (P<0.05). Expressions of TLR-2 and TLR-4 in chronic periodontitis group were significantly higher than those in aggressive periodontitis group (P<0.05). Expression of TLR-4 in chronic periodontitis clinically healthy group was significantly higher than that in aggressive periodontitis clinically healthy group (P<0.05). But expression of TLR-2 in chronic periodontitis clinically healthy group was significantly lower than that in aggressive periodontitis clinically healthy group (P<0.05). Conclusion TLR-2 and TLR-4 might be involved in the progress of periodontal inflammation. But expressions of TLR-2 and TLR-4 in gingival tissues from different kinds periodontitis may be different.

Key words: Toll-like receptor, chronic periodontitis, aggressive periodontitis, gingiva

中图分类号: