上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (2): 240-.

• 技术与方法 • 上一篇    下一篇

血管紧张素转化酶底物的制备及对肺癌诊断的临床意义

周海燕1, 姚莉韵2, 李盈懿3, 张建华2   

  1. 上海交通大学 1. 胸科医院检验科, 上海 200030, 2. 基础医学院化学教研室, 上海 200025, 3. 医学院辅导员办公室, 上海 200025
  • 出版日期:2010-02-25 发布日期:2010-02-25
  • 通讯作者: 姚莉韵, 电子信箱: yaoliyun517@yahoo.com.cn。
  • 作者简介:周海燕（1970—）, 女, 检验师；电子信箱: zhouhy180@sina.com。
  • 基金资助:

    上海市卫生局科技发展基金项目 （2007085）

Synthesis of substrate for angiotensin-converting enzyme and its value in diagnosis of lung cancer

ZHOU Hai-yan1, YAO Li-yun2, LI Ying-yi3, ZHANG Jian-hua2   

  1. 1. Department of Clinical Laboratory, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, China；2. Department of Chemistry, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China；3. Office of Counselor, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2010-02-25 Published:2010-02-25
  • Supported by:

    Shanghai Municipal Health Bureau Foundation, 2007085

PDF (PC)

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摘要：

目的 建立血管紧张素转化酶（ACE）底物的合成方法，探讨血清ACE活性的测定方法。方法 底物制备采用N-羟基琥珀酰亚胺活化酯合成多肽法；建立分光光度法测定ACE的活性。结果 底物呋喃丙烯酰苯丙氨酰甘氨酰甘氨酸纯度为99.5%。底物的米氏常数Km为0.25 mmol/L，血清ACE活性正常参考值范围46.7～113.3 U/L，标本批内和批间平均变异系数分别为3.9%和4.9%。临床检测表明，肺鳞癌、肺腺癌患者血清ACE活性显著下降（P<0.05）；而肺小细胞癌患者ACE活性没有明显降低（P>0.05）。结论 所合成的底物适用于临床诊断各类肺癌的要求。

关键词: 血管紧张素转化酶, 呋喃丙烯酰苯丙氨酰甘氨酰甘氨酸, 肺癌

Abstract:

Objective To establish the synthetic method of the substrate for angiotensin-converting enzyme (ACE), and investigate the assay method of serum ACE. Methods The substrate was prepared by the use of N-hydroxysuccinimide ester in peptide synthesis, and serum ACE was determined by spectrophotometric assay using substrate. Results The substrate of furanacryloyl-phenylalanyl-glycyl-glycine with purity of 99.5% was obtained. The Km value for the substrate was 0.25 mmol/L, the normal reference interval for serum ACE activity was 46.7 U/L to 113.3 U/L, and the within-run and between-run coefficient of variation (CVs) ranged from 3.9 to 4.9%. The serum ACE activity in patients with squamous carcinoma and adenocarcinoma of lung were significantly decreased (P<0.05), while that in patients with lung small cell carcinoma was not significantly decreased (P>0.05). Conclusion The synthesized substrate could be reliably used as a marker for diagnosis of different lung cancers in clinics.

Key words: angiotensin converting enzyme, furanacryloyl-phenylalanyl-glycyl-glycine, lung cancer