上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (4): 390-.

• 论著（基础研究） • 上一篇    下一篇

罗格列酮对高糖环境中3T3-L1脂肪细胞血管紧张素Ⅱ受体基因表达的调控作用

徐 茜, 张志国, 金丽娜, 石国军, 杨 洋, 田秀丽, 张杉杉, 崔 斌, 汤正义, 宁 光   

  1. 上海交通大学 医学院瑞金医院内分泌代谢病科 上海市内分泌代谢病临床医学中心 上海市内分泌代谢病研究所, 上海 200025
  • 出版日期:2010-04-25 发布日期:2010-04-26
  • 通讯作者: 汤正义, 电子信箱: tsy2zyp@163.com。
  • 作者简介:徐 茜（1982—）, 女, 硕士生；电子信箱: sissixu@163.com。
  • 基金资助:

    上海市内分泌肿瘤重点实验室基金（08DZ2271000）

Regulation of rosiglitazone on expression of angiotension Ⅱ receptor genes in 3T3-L1 adipocytes under high glucose concentrations

XU Qian, ZHANG Zhi-guo, JIN Li-na, SHI Guo-jun, YANG Yang, TIAN Xiu-li, ZHANG Shan-shan, CUI Bin, TANG Zheng-yi, NING Guang   

  1. Department of Endocrine and Metabolic Diseases, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2010-04-25 Published:2010-04-26
  • Supported by:

    Foundation of Shanghai Key Laboratory of Endocrine Tumor, 08DZ2271000

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摘要：

目的 研究不同浓度葡萄糖培养环境及罗格列酮干预对分化成熟脂肪细胞血管紧张素Ⅱ受体1 (AT1R)和受体2 (AT2R) 基因表达的影响。方法 取分化成熟的脂肪细胞3T3-L1，分别用含不同浓度葡萄糖（空白对照、5.6、11.2、25.0 mmol/L）的培养基和含不同浓度葡萄糖并添加10 nmol/L罗格列酮的培养基分组培养24 h, Real-time PCR检测3T3-L1脂肪细胞AT1R和AT2R基因表达。结果 5.6 mmol/L葡萄糖处理组AT1R基因表达明显低于空白对照和11.2、25.0 mmol/L葡萄糖处理组（P<0.05）；AT2R基因表达随着葡萄糖浓度的升高而明显上调（P<0.05）。随着葡萄糖浓度的升高，罗格列酮干预组AT1R和AT2R基因表达呈下降趋势。与相应浓度的葡萄糖处理组比较，罗格列酮干预组AT1R表达均显著上调 (P<0.05)，作用随葡萄糖浓度的升高而减弱；AT2R基因表达显著下调(P<0.05)，作用随葡萄糖浓度的升高而加强。结论 罗格列酮对高浓度葡萄糖导致的脂肪细胞AT1R和AT2R基因表达变化具有调控作用。

关键词: 葡萄糖, 血管紧张素Ⅱ受体, 罗格列酮, 脂肪细胞

Abstract:

Objective To investigate the effects of different glucose concentrations and rosiglitazone intervention on expression of angiotension Ⅱ receptor 1 (AT1R) and angiotension Ⅱ receptor 2 (AT2R) genes in differentiated mature adipocytes. Methods Differentiated mature 3T3-L1 adipocytes were cultured in media with different concentrations of glucose (blank control, 5.6 mmol/L, 11.2 mmol/L and 25.0 mmol/L) and media with different concentrations of glucose and 10 nmol/L rosiglitazone for 24 h. Real-time PCR was employed to detect the expression of AT1R and AT2R genes in 3T3-L1 adipocytes. Results The expression of AT1R gene was significantly lower in 5.6 mmol/L glucose treatment group than that in blank control group, 11.2 mmol/L glucose treatment group and 25.0 mmol/L glucose treatment group (P<0.05), and the expression of AT2R gene significantly increased with the increase of glucose concentrations (P<0.05). The expression of AT1R and AT2R genes in rosiglitazone intervention group decreased with the increase of glucose concentrations. Compared with corresponding glucose treatment groups, the expression of AT1R gene in rosiglitazone intervention groups was significantly up-regulated (P<0.05), with the effect decreasing gradually with the increase of glucose concentrations, and the expression of AT2R gene was significantly down-regulated (P<0.05), with the effect increasing with the increase of glucose concentrations. Conclusion Rosiglitazone plays a regulatory role in the expression of AT1R and AT2R genes in adipocytes under high concentrations of glucose.

Key words: glucose, angiotesin Ⅱ receptor, rosiglitazone, adipocytes